Paxillin ? and Crk-associated substrate exert opposing effects on cell migration and contact inhibition of growth through tyrosine phosphorylation
|
Descargar SCORM
¡Sea el primero en solicitar este recurso!
Para poder solicitar este recurso debe identificarse como usuario de la biblioteca
|
| |
Ver
Detalles del recurso
|
|
|
Paxillin ? and Crk-associated substrate exert opposing effects on cell migration and contact inhibition of growth through tyrosine phosphorylation
|
| Id. |
17583411 |
| Idioma |
inglés
|
| Titulo |
Paxillin ? and Crk-associated substrate exert opposing effects on cell migration and contact inhibition of growth through tyrosine phosphorylation |
| Autor(es) |
Yano, Hajime
Uchida, Hiroshi
Iwasaki, Teruo
Mukai, Mutsuko
Akedo, Hitoshi
Nakamura, Kuniaki
Hashimoto, Shigeru
Sabe, Hisataka
|
| Localización |
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=16824
|
| Versión |
1.0 |
| Estado |
Final
|
| Descripción |
Protein tyrosine phosphorylation accompanies and is essential for integrin signaling. We have shown that tyrosine phosphorylation of paxillin ? and Crk-associated substrate (p130Cas) is a prominent event on integrin activation in normal murine mammary gland epithelial cells. Tyrosine phosphorylation of p130Cas has been demonstrated to facilitate cell migration. We show here that tyrosine phosphorylation of paxillin ? acts to reduce haptotactic cell migrations as well as transcellular invasive activities in several different experimental cell systems, whereas tyrosine phosphorylation of p130Cas exerts opposing effects to those of paxillin ?. Each of the phosphorylation-null mutants acts as a dominant negative for each phenotype. Moreover, we found that overexpression of paxillin ? reduced the cell saturation density of normal murine mammary gland cells, whereas overexpression of p130Cas increased it. These effects also seemed to depend on tyrosine phosphorylation events. Cell growth rates and morphologies at growing phases were not significantly altered, nor were cells transformed. Addition of epidermal growth factor increased saturation density of the paxillin ?-overexpressing cells, whereas no further increment was observed in p130Cas-overexpressing cells. We propose that tyrosine phosphorylation of paxillin ? and p130Cas exerts opposing effects on several integrin-mediated cellular events, possibly through different signaling pathways.
|
| Palabras clave |
Biological Sciences
|
| Tipo de recurso |
Text
|
| Tipo de Interactividad |
Expositivo
|
| Nivel de Interactividad |
muy bajo
|
| Audiencia |
Estudiante
Profesor
Autor
|
| Estructura |
Atomic |
| Coste |
no
|
| Copyright |
sí
|
|
Copyright © 2000, The National Academy of Sciences |
| Requerimientos técnicos |
Browser: Any
|
| Fecha de contribución |
05-feb-2008 |
| Contacto |
|
|
|
|
|
Valoración de los usuarios
No hay ninguna valoración para este recurso. Sea el primero en
valorar este recurso.
|
|
|
|
