Cleavage of the human respiratory syncytial virus fusion protein
at two distinct sites is required for activation of membrane
fusion
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Cleavage of the human respiratory syncytial virus fusion protein
at two distinct sites is required for activation of membrane
fusion
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| Id. |
17628265 |
| Idioma |
inglés
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| Titulo |
Cleavage of the human respiratory syncytial virus fusion protein
at two distinct sites is required for activation of membrane
fusion |
| Autor(es) |
González-Reyes, Luis
Ruiz-Argüello, M. Begoña
GarcÃa-Barreno, Blanca
Calder, Leslie
López, Juan A.
Albar, Juan P.
Skehel, John J.
Wiley, Don C.
Melero, José A.
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| Localización |
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=55543
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| Versión |
1.0 |
| Estado |
Final
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| Descripción |
Preparations of purified full-length fusion (F) protein of human
respiratory syncytial virus (HRSV) expressed in recombinant vaccinia-F
infected cells, or of an anchorless mutant (FTMâ??)
lacking the C-terminal 50 amino acids secreted from
vaccinia-FTMâ??-infected cells contain a minor
polypeptide that is an intermediate product of proteolytic processing
of the F protein precursor F0. N-terminal sequencing of the
intermediate demonstrated that it is generated by cleavage at a
furin-motif, residues 106â??109 of the F sequence. By contrast, the F1 N
terminus derives from cleavage at residue 137 of F0 which is also
C-terminal to a furin recognition site at residues 131â??136.
Site-directed mutagenesis indicates that processing of F0 protein
involves independent cleavage at both sites. Both cleavages are
required for the F protein to be active in membrane fusion as judged by
syncytia formation, and they allow changes in F structure from cone- to
lollipop-shaped spikes and the formation of rosettes by anchorless F.
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| Palabras clave |
Biological Sciences
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| Tipo de recurso |
Text
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| Tipo de Interactividad |
Expositivo
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| Nivel de Interactividad |
muy bajo
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| Audiencia |
Estudiante
Profesor
Autor
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| Estructura |
Atomic |
| Coste |
no
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| Copyright |
sí
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Copyright © 2001, The National Academy of Sciences |
| Requerimientos técnicos |
Browser: Any
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| Fecha de contribución |
08-feb-2008 |
| Contacto |
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