Effects of different preservation temperatures and periods menisci cellularity in rabbits
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Effects of different preservation temperatures and periods menisci cellularity in rabbits
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| Id. |
2099381 |
| Idioma |
inglés
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| Titulo |
Effects of different preservation temperatures and periods menisci cellularity in rabbits |
| Autor(es) |
Reckers,Leandro José
Fagundes,Djalma José
Cohen,Moisés
Raymundo,José Luiz Pozzo
Moreira,Márcia Bento
Paiva,Vanessa Carla
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| Localización |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502005000600006
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| Versión |
1.0 |
| Estado |
Final
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| Descripción |
PURPOSE: Quantify the progressive decrease of the cellular viability of rabbit meniscus preserved for transplants over a 30 day period at different freezing temperatures. METHODS: 180 menisci were removed from 45 rabbits. Menisci were frozen from 2 to 30 days at -7.2°Celsius -21.4°Celsius and -73°Celsius. Four menisci from each temperature were thawed every two days and the number of present cells was quantified. RESULTS: On the 14th freezing day at -7.2°Celsius, there were 92.38% mean viable cells. However, as from the 16th day, there has been a significant 12% decrease (p = 0.001), as compared to 14th day mean. Mean cell viability at -21.4°Celsius and -73ºCelsius, until the 16th day was statistically similar. As from the 18th day at -21.4°Celsius, there has been significant cell count decrease (p < 0.001), especially from the 28th (54.5%) to the 30th (30%) freezing day. The number of viable cells at -73 0Celsius has shown insignificant 2.3% decrease (p = 1.000) from the 28th (40.2%) to the 30th (37.9%) day. CONCLUSION: The amount of viable meniscus cells is similar up to the 14th day of preservation, under any of the used temperatures. However, from the 14th day to the 30th day, the amount of viable cells is smaller in lower temperatures.
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| Tipo |
text/html
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| Palabras clave |
Freezing
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| Tipo de recurso |
journal article
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| Tipo de Interactividad |
Expositivo
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| Nivel de Interactividad |
muy bajo
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| Audiencia |
Estudiante
Profesor
Autor
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| Estructura |
Atomic |
| Coste |
no
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| Copyright |
sí
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| Formatos |
text/html
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| Requerimientos técnicos |
Browser: Any
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| Fecha de contribución |
02-dic-2005 |
| Contacto |
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