The fidelity of DNA synthesis by yeast DNA polymerase zeta alone and with accessory proteins
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The fidelity of DNA synthesis by yeast DNA polymerase zeta alone and with accessory proteins
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| Id. |
16417720 |
| Idioma |
inglés
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| Titulo |
The fidelity of DNA synthesis by yeast DNA polymerase zeta alone and with accessory proteins |
| Autor(es) |
Zhong, Xuejun
Garg, Parie
Stith, Carrie M.
McElhinny, Stephanie A. Nick
Kissling, Grace E.
Burgers, Peter M. J.
Kunkel, Thomas A.
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| Localización |
http://www.pubmedcentral.gov/articlerender.fcgi?artid=1635245
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| Versión |
1.0 |
| Estado |
Final
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| Descripción |
DNA polymerase zeta (pol ?) participates in several DNA transactions in eukaryotic cells that increase spontaneous and damage-induced mutagenesis. To better understand this central role in mutagenesis in vivo, here we report the fidelity of DNA synthesis in vitro by yeast pol ? alone and with RFC, PCNA and RPA. Overall, the accessory proteins have little effect on the fidelity of pol ?. Pol ? is relatively accurate for single base insertion/deletion errors. However, the average base substitution fidelity of pol ? is substantially lower than that of homologous B family pols ?, ? and ?. Pol ? is particularly error prone for substitutions in specific sequence contexts and generates multiple single base errors clustered in short patches at a rate that is unprecedented in comparison with other polymerases. The unique error specificity of pol ? in vitro is consistent with Pol ?-dependent mutagenic specificity reported in vivo. This fact, combined with the high rate of single base substitution errors and complex mutations observed here, indicates that pol ? contributes to mutagenesis in vivo not only by extending mismatches made by other polymerases, but also by directly generating its own mismatches and then extending them.
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| Palabras clave |
Nucleic Acid Enzymes
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| Tipo de recurso |
Text
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| Tipo de Interactividad |
Expositivo
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| Nivel de Interactividad |
muy bajo
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| Audiencia |
Estudiante
Profesor
Autor
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| Estructura |
Atomic |
| Coste |
no
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| Copyright |
sí
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Published by Oxford University Press 2006 |
| Requerimientos técnicos |
Browser: Any
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| Fecha de contribución |
03-dic-2006 |
| Contacto |
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