Skipping the co-expression problem: the new 2A "CHYSEL" technology
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Skipping the co-expression problem: the new 2A "CHYSEL" technology
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| Id. |
18017688 |
| Idioma |
inglés
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| Titulo |
Skipping the co-expression problem: the new 2A "CHYSEL" technology |
| Autor(es) |
de Felipe, Pablo
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| Localización |
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=521497
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| Versión |
1.0 |
| Estado |
Final
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| Descripción |
The rapid progress in the field of genomics is increasing our knowledge of multi-gene diseases. However, any realistic hope of gene therapy treatment for those diseases needs first to address the problem of co-ordinately co-expressing several transgenes. Currently, the use of internal ribosomal entry sites (IRESs) is the strategy chosen by many researchers to ensure co-expression. The large sizes of the IRESs (~0.5 kb), and the difficulties of ensuring a well-balanced co-expression, have prompted several researchers to imitate a co-expression strategy used by many viruses: to express several proteins as a polyprotein. A small peptide of 18 amino acids (2A) from the foot-and-mouth disease virus (FMDV) is being used to avoid the need of proteinases to process the polyprotein. FMDV 2A is introduced as a linker between two proteins to allow autonomous intra-ribosomal self-processing of polyproteins. Recent reports have shown that this sequence is compatible with different sub-cellular targeting signals and can be used to co-express up to four proteins from a single retroviral vector. This short peptide provides a tool to allow the co-expression of multiple proteins from a single vector, a useful technology for those working with heteromultimeric proteins, biochemical pathways or combined/synergistic phenomena.
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| Palabras clave |
Commentary
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| Tipo de recurso |
Text
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| Tipo de Interactividad |
Expositivo
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| Nivel de Interactividad |
muy bajo
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| Audiencia |
Estudiante
Profesor
Autor
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| Estructura |
Atomic |
| Coste |
no
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| Copyright |
sí
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| Requerimientos técnicos |
Browser: Any
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| Fecha de contribución |
04-mar-2008 |
| Contacto |
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