Publicidad

Publicidad

becas.universia.netBiblioteca.Net

Buscar recursos:

Buscador Google

Resource data



Ver

Off-lattice simulation of the solid phase DNA amplification
Krawczyk, M. J.
Kulakowski, K.
Location: http://arxiv.org/abs/q-bio/0412009

Recent simulations of the solid phase DNA amplification (SPA) by J.-F. Mercier et al (Biophys. J. 85 (2003) 2075) are generalized to include two kinds of primers and the off-lattice character of the primer distribution on the surface. The sigmoidal character of the primer occupation by DNA, observed experimentally, is reproduced in the simulation. We discuss an influence of two parameters on the efficience of the amplification process: the initial density p_0 of the occupied primers from the interfacial amplification and the ratio r of the molecule length to the average distance between primers. The number of cycles till the saturation decreases with p_0 roughly as p_0^{-0.26}. For r=1.5, the number of occupied primers is reduced by a factor two, when compared to the case of longer molecules. Below r=1.4, the effectivity of SPA is reduced by a factor 100.

Belongs to: arXiv

Descargar SCORM

¡Sea el primero en solicitar este recurso!

Para poder solicitar este recurso debe identificarse como usuario de la biblioteca

Users rating

No hay ninguna valoración para este recurso. Sea el primero en valorar este recurso.

Detalles del recurso

Off-lattice simulation of the solid phase DNA amplification
Id. 20880371
Titulo Off-lattice simulation of the solid phase DNA amplification
Autor(es) Krawczyk, M. J.
Kulakowski, K.
Location http://arxiv.org/abs/q-bio/0412009
Versión 1.0
Estado Final
Descripción Recent simulations of the solid phase DNA amplification (SPA) by J.-F. Mercier et al (Biophys. J. 85 (2003) 2075) are generalized to include two kinds of primers and the off-lattice character of the primer distribution on the surface. The sigmoidal character of the primer occupation by DNA, observed experimentally, is reproduced in the simulation. We discuss an influence of two parameters on the efficience of the amplification process: the initial density p_0 of the occupied primers from the interfacial amplification and the ratio r of the molecule length to the average distance between primers. The number of cycles till the saturation decreases with p_0 roughly as p_0^{-0.26}. For r=1.5, the number of occupied primers is reduced by a factor two, when compared to the case of longer molecules. Below r=1.4, the effectivity of SPA is reduced by a factor 100.
Palabras clave Quantitative Biology - Biomolecules
Tipo de recurso Texto Narrativo
Tipo de Interactividad Expositivo
Nivel de Interactividad muy bajo
Audiencia Estudiante
Profesor
Autor
Estructura Atomic
Coste no
Copyright
Requerimientos técnicos Browser: Any
Fecha de contribución 28-mar-2007
Contacto