Off-lattice simulation of the solid phase DNA amplification
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Off-lattice simulation of the solid phase DNA amplification
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| Id. |
20880371 |
| Titulo |
Off-lattice simulation of the solid phase DNA amplification |
| Autor(es) |
Krawczyk, M. J.
Kulakowski, K.
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| Localización |
http://arxiv.org/abs/q-bio/0412009
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| Versión |
1.0 |
| Estado |
Final
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| Descripción |
Recent simulations of the solid phase DNA amplification (SPA) by J.-F.
Mercier et al (Biophys. J. 85 (2003) 2075) are generalized to include two kinds
of primers and the off-lattice character of the primer distribution on the
surface. The sigmoidal character of the primer occupation by DNA, observed
experimentally, is reproduced in the simulation. We discuss an influence of two
parameters on the efficience of the amplification process: the initial density
p_0 of the occupied primers from the interfacial amplification and the ratio r
of the molecule length to the average distance between primers. The number of
cycles till the saturation decreases with p_0 roughly as p_0^{-0.26}. For
r=1.5, the number of occupied primers is reduced by a factor two, when compared
to the case of longer molecules. Below r=1.4, the effectivity of SPA is reduced
by a factor 100.
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| Palabras clave |
Quantitative Biology - Biomolecules
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| Tipo de recurso |
Texto Narrativo
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| Tipo de Interactividad |
Expositivo
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| Nivel de Interactividad |
muy bajo
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| Audiencia |
Estudiante
Profesor
Autor
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| Estructura |
Atomic |
| Coste |
no
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| Copyright |
sí
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| Requerimientos técnicos |
Browser: Any
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| Fecha de contribución |
28-mar-2007 |
| Contacto |
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