Biodegradabilidade de isômeros de xileno por bactérias isoladas determinal portuário de SUAPE-PE
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Biodegradabilidade de isômeros de xileno por bactérias isoladas determinal portuário de SUAPE-PE
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| Id. |
30826675 |
| Idioma |
PT
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| Titulo |
Biodegradabilidade de isômeros de xileno por bactérias isoladas determinal portuário de SUAPE-PE |
| Autor(es) |
Laureni Alves de Lima
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| Localización |
http://www.bdtd.ufpe.br/tedeSimplificado//tde_busca/arquivo.php?codArquivo=2541
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| Versión |
1.0 |
| Estado |
Final
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| Descripción |
Xylene, also known as dimethylbenzene is a monoaromatic hydrocarbon existing in three isometric forms: ortho, meta and para. It is widely used in paints, glues, printer ink, pesticides, in the leather and rubber industries, in histology labs and is a component of gasoline. The constant use of this compound may cause damage in the environment and to human health, owing to its toxicity. An alternative way of eliminating or minimizing these problems, is bioremediation, a technique that uses microorganisms to degrade pollutants. This study aims to investigate the biodegradability of xylene isomers by bacteria isolated from the Port Terminal at Suape in the Brazilian State of Pernambuco. Forty bacterial strains, previously isolated from an environment polluted by petroleum derivatives, were submitted to selection test of cultures, isolated or consortia, which have the potential to degrade xylene isomers or a mixture of them, using the technique involving the redox indicator 2,6 dichlorophenol indophenol. The selected strain was identified and acclimated by varying the concentration of the oily source (1%, 3% and 5% v/v) in a Bushnell-Haas (BH) mineral medium. The biodegradation assays were carried out in Erlenmeyer flasks (500 mL), sealed with rubber corks, containing 75% of BH medium, 20% of inoculum acclimated and 5% of the carbon source. These flasks were incubated at temperature of 30±1°C and shaken at 200 rpm for 24 and 72 hours; the cellular concentration and the percentage of biodegradation were measured at these times. Of the forty strains tested, UFPEDA-816 showed the potential to degrade all three xylene isomers, while UFPEDA-837 was capable of oxidizing only the ortho- and meta-xylene isomers; and the consortium comprising these two strains did not show potential to degrade these isomers. The UFPEDA-816 strain was identified as Bacillus megaterium. On the acclimatation assays, the pH values remained near toneutrality; the selected bacteria showed the greatest growth in the presence of ortho-xylene and in the mixture of isomers; and there was a greater reduction in the surface tension of the medium when para-xylene was used as the oily source. On the biodegradation assays were observed that, after 24h, there was significant growth of B. megaterium with ortho-xylene and the mixture of isomers; after 72h, there was a reduction in cellular growth in the presence of all carbon sources. The highest percentage of biodegradation occurred in the first 24h, being significant only to ortho-xylene
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| Tipo |
PDF
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| Palabras clave |
Bactérias
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| Tipo de recurso |
Electronic Thesis or Dissertation
Tese ou Dissertacao Eletronica
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| Tipo de Interactividad |
Expositivo
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| Nivel de Interactividad |
muy bajo
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| Audiencia |
Estudiante
Profesor
Autor
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| Estructura |
Atomic |
| Coste |
no
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| Copyright |
sí
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Reter o conteúdo dos arquivos por motivos de patente, publicação e/ou direitos autorais |
| Formatos |
PDF
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| Requerimientos técnicos |
Browser: Any
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| Fecha de contribución |
23-feb-2009 |
| Contacto |
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