Structure-function study of maize ribosome-inactivating protein: implications for the internal inactivation region and the sole glutamate in the active site
|
Descargar SCORM
Este recurso ha sido solicitado 1 veces (0 veces en los últimos 31 días).
Para poder solicitar este recurso debe identificarse como usuario de la biblioteca
|
| |
Ver
Detalles del recurso
|
|
|
Structure-function study of maize ribosome-inactivating protein: implications for the internal inactivation region and the sole glutamate in the active site
|
| Id. |
31418735 |
| Idioma |
inglés
|
| Titulo |
Structure-function study of maize ribosome-inactivating protein: implications for the internal inactivation region and the sole glutamate in the active site |
| Autor(es) |
Mak, Amanda Nga-Sze
Wong, Yuen-Ting
An, Young-Jun
Cha, Sun-Shin
Sze, Kong-Hung
Au, Shannon Wing-Ngor
Wong, Kam-Bo
Shaw, Pang-Chui
|
| Localización |
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2094058
|
| Versión |
1.0 |
| Estado |
Final
|
| Descripción |
Maize ribosome-inactivating protein is classified as a class III or an atypical RNA N-glycosidase. It is synthesized as an inactive precursor with a 25-amino acid internal inactivation region, which is removed in the active form. As the first structural example of this class of proteins, crystals of the precursor and the active form were diffracted to 2.4 and 2.5 Å, respectively. The two proteins are similar, with main chain root mean square deviation (RMSD) of 0.519. In the precursor, the inactivation region is found on the protein surface and consists of a flexible loop followed by a long ?-helix. This region diminished both the interaction with ribosome and cytotoxicity, but not cellular uptake. Like bacterial ribosome-inactivating proteins, maize ribosome-inactivating protein does not have a back-up glutamate in the active site, which helps the protein to retain some activity if the catalytic glutamate is mutated. The structure reveals that the active site is too small to accommodate two glutamate residues. Our structure suggests that maize ribosome-inactivating protein may represent an intermediate product in the evolution of ribosome-inactivating proteins.
|
| Palabras clave |
Nucleic Acid Enzymes
|
| Tipo de recurso |
Text
|
| Tipo de Interactividad |
Expositivo
|
| Nivel de Interactividad |
muy bajo
|
| Audiencia |
Estudiante
Profesor
Autor
|
| Estructura |
Atomic |
| Coste |
no
|
| Copyright |
sí
|
|
© 2007 The Author(s) |
| Requerimientos técnicos |
Browser: Any
|
| Fecha de contribución |
13-mar-2008 |
| Contacto |
|
|
|
|
|
Valoración de los usuarios
No hay ninguna valoración para este recurso. Sea el primero en
valorar este recurso.
|
|
|
|
