Development and standardization of an indirect ELISA for the serological diagnosis of classical swine fever
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Development and standardization of an indirect ELISA for the serological diagnosis of classical swine fever
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| Id. |
505202 |
| Idioma |
inglés
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| Titulo |
Development and standardization of an indirect ELISA for the serological diagnosis of classical swine fever |
| Autor(es) |
Paredes,Julio Cesar Muñoz
Oliveira,Liliane Guimarães
Braga,Alexandre de Carvalho
Trevisol,Iara Maria
Roehe,Paulo Michel
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| Localización |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X1999000300006
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| Versión |
1.0 |
| Estado |
Final
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| Descripción |
An indirect enzyme linked immunoassay (ELISA-I) was developed and standardized for the serological diagnosis of classical swine fever (CSF). For the comparison, nine hundred and thirty-seven swine serum samples were tested by serum neutralization followed by immunoperoxidase staining (NPLA), considered as the standard. Of these, 223 were positive and 714 negative for neutralizing antibodies to classical swine fever virus (CSFV). In relation to the NPLA, the ELISA-I presented a 98.2% sensitivity; 92.86% specificity, 81.11% positive predictive value, 99.4% negative predictive value and a 94.1% precision. Statistical analysis showed a very strong correlation (r=0,94) between both tests. When compared to a commercially available ELISA kit, the performance of both, in relation to the NPLA, was similar. It was concluded that the ELISA-I is suitable for large scale screening of antibodies to classical swine fever virus, although it does not distinguish antibodies to classical swine fever virus from those induced by other pestiviruses.
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| Tipo |
text/html
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| Palabras clave |
Classical swine fever
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| Tipo de recurso |
journal article
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| Tipo de Interactividad |
Expositivo
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| Nivel de Interactividad |
muy bajo
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| Audiencia |
Estudiante
Profesor
Autor
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| Estructura |
Atomic |
| Coste |
no
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| Copyright |
sí
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| Formatos |
text/html
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| Requerimientos técnicos |
Browser: Any
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| Fecha de contribución |
22-may-2005 |
| Contacto |
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