The Slow Wallerian Degeneration Protein, WldS, Binds Directly to VCP/p97 and Partially Redistributes It within the NucleusD?
|
Descargar SCORM
Este recurso ha sido solicitado 1 veces (0 veces en los últimos 31 días).
Para poder solicitar este recurso debe identificarse como usuario de la biblioteca
|
| |
Ver
Detalles del recurso
|
|
|
The Slow Wallerian Degeneration Protein, WldS, Binds Directly to VCP/p97 and Partially Redistributes It within the NucleusD?
|
| Id. |
5313261 |
| Idioma |
inglés
|
| Titulo |
The Slow Wallerian Degeneration Protein, WldS, Binds Directly to VCP/p97 and Partially Redistributes It within the NucleusD? |
| Autor(es) |
Laser, Heike
Conforti, Laura
Morreale, Giacomo
Mack, Till G.M.
Heyer, Molly
Haley, Jane E.
Wishart, Thomas M.
Beirowski, Bogdan
Walker, Simon A.
Haase, Georg
Celik, Arzu
Adalbert, Robert
Wagner, Diana
Grumme, Daniela
Ribchester, Richard R.
Plomann, Markus
Coleman, Michael P.
|
| Localización |
http://www.pubmedcentral.gov/articlerender.fcgi?artid=1382299
|
| Versión |
1.0 |
| Estado |
Final
|
| Descripción |
Slow Wallerian degeneration (WldS) mutant mice express a chimeric nuclear protein that protects sick or injured axons from degeneration. The C-terminal region, derived from NAD+ synthesizing enzyme Nmnat1, is reported to confer neuroprotection in vitro. However, an additional role for the N-terminal 70 amino acids (N70), derived from multiubiquitination factor Ube4b, has not been excluded. In wild-type Ube4b, N70 is part of a sequence essential for ubiquitination activity but its role is not understood. We report direct binding of N70 to valosin-containing protein (VCP; p97/Cdc48), a protein with diverse cellular roles including a pivotal role in the ubiquitin proteasome system. Interaction with WldS targets VCP to discrete intranuclear foci where ubiquitin epitopes can also accumulate. WldS lacking its N-terminal 16 amino acids (N16) neither binds nor redistributes VCP, but continues to accumulate in intranuclear foci, targeting its intrinsic NAD+ synthesis activity to these same foci. Wild-type Ube4b also requires N16 to bind VCP, despite a more C-terminal binding site in invertebrate orthologues. We conclude that N-terminal sequences of WldS protein influence the intranuclear location of both ubiquitin proteasome and NAD+ synthesis machinery and that an evolutionary recent sequence mediates binding of mammalian Ube4b to VCP.
|
| Palabras clave |
Articles
|
| Tipo de recurso |
Text
|
| Tipo de Interactividad |
Expositivo
|
| Nivel de Interactividad |
muy bajo
|
| Audiencia |
Estudiante
Profesor
Autor
|
| Estructura |
Atomic |
| Coste |
no
|
| Copyright |
sí
|
|
Copyright © 2006, The American Society for Cell Biology |
| Requerimientos técnicos |
Browser: Any
|
| Fecha de contribución |
01-dic-2006 |
| Contacto |
|
|
|
|
|
Valoración de los usuarios
No hay ninguna valoración para este recurso. Sea el primero en
valorar este recurso.
|
|
|
|
