Hemagglutination by Rabies Virus
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Hemagglutination by Rabies Virus
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| Id. |
788232 |
| Idioma |
inglés
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| Titulo |
Hemagglutination by Rabies Virus |
| Autor(es) |
Kuwert, Ernst
Wiktor, Tadeusz J.
Sokol, Frantisek
Koprowski, Hilary
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| Localización |
http://www.pubmedcentral.gov/articlerender.fcgi?artid=375483
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| Versión |
1.0 |
| Estado |
Final
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| Descripción |
Goose erythrocytes were agglutinated by five strains of rabies virus grown in monolayer cell cultures at pH 6.4 and at 0 to 4 C. Hemagglutination was not affected by the cell type in which the virus was grown. Prerequisites for occurrence of hemagglutination are absence of hemagglutination inhibitors (such as those contained in bovine serum) and a relatively high virus concentration (> 106 plaque-forming units of virus per ml). Soluble hemagglutinin was not present in crude preparations of extracellular virus. Treatment of purified preparations of extracellular virus with Tween 80 and ether did not result in release of a soluble hemagglutinin. The hemagglutinating property of extracellular virus seemed to be conditioned by the integrity of its coat. Preparations of infectious intracellular virus exhibited about 15 times lower hemagglutinating activity than extracellular virus. This decreased hemagglutinating activity did not seem to be caused by binding of hemagglutination inhibitors to the virus particles. Rabies virus can be quantitatively adsorbed onto and eluted from erythrocytes. Erythrocytes pretreated with rabies virus retained their ability to be agglutinated by the same virus strain. The reaction with rabies virus of erythrocytes treated with the receptor-destroying enzyme or KIO4 was the same as that of nontreated erythrocytes. The hemagglutinating component of rabies virus, therefore, does not exhibit neuraminidase activity. Treatment of extracellular virus by various agents indicated that the hemagglutinating component consists of protein or lipoprotein. Sulfhydryl groups present in the viral hemagglutinin are essential for hemagglutination.
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| Palabras clave |
Animal Viruses
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| Tipo de recurso |
Text
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| Tipo de Interactividad |
Expositivo
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| Nivel de Interactividad |
muy bajo
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| Audiencia |
Estudiante
Profesor
Autor
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| Estructura |
Atomic |
| Coste |
no
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| Copyright |
sí
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Copyright © 1968 American Society for Microbiology |
| Requerimientos técnicos |
Browser: Any
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| Fecha de contribución |
22-nov-2006 |
| Contacto |
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