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17α-hydroxy-4-pregnen-3-one (17αP) hormone is a precursor of other steroid hormones and radioimmunoassay has already been used to measure it, but a simple and rapid "Enzyme-Linked Immunosorbent Assay" (ELISA) is described and validated here. A general procedure for preparation of the acetylcholinesterase labelled steroid is described, which is applicable to any steroid. Use of acetylcholinesterase tracer increased the sensitivity of assay so that reliable measurements of each steroid could be achieved with only 10 ml of plasma. The ELISA was applied to measure 17, 20α-hydroxy-4-pregnen-3-one (17, 20αP) steroid production from 17αP by 20 α-hydroxy steroid dehydrogenase (20α-HSD) from sperm of roach (Rutilus rutilus). The results showed that cyprinid sperm contains potent and active 20α-HSD enzymes which produce 17, 20αP hormone from 17αP substrate.

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Aquatic Commons  


Ebrahimi, M. - 

Id.: 71057741

Idioma: inglés  - 

Versión: 1.0

Estado: Final

Tipo:  application/pdf - 

Palabras claveBiology - 

Tipo de recurso: Article  -  PeerReviewed  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

Formatos:  application/pdf - 

Requerimientos técnicos:  Browser: Any - 

Relación: [References] http://aquaticcommons.org/22272/

Fecha de contribución: 08-mar-2018


* Ebrahimi, M. (2005) 17α-hydroxy-4-pregenen-3-one (17αP) assay, using acetylcholinesterase enzyme as tracer. Iranian Journal of Fisheries Sciences, 5(1), pp. 13-28.

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