1) La descarga del recurso depende de la página de origen
2) Para poder descargar el recurso, es necesario ser usuario registrado en Universia

Opción 1: Descargar recurso

Detalles del recurso


Malaria is transmitted by Plasmodium-infected anopheles mosquitoes. Widespread resistance of mosquitoes to insecticides and resistance of parasites to drugs highlight the urgent need for malaria vaccines. The most advanced malaria vaccines target sporozoites, the infective form of the parasite. A major target of the antibody response to sporozoites are the repeat epitopes of the circumsporozoite (CS) protein, which span almost one half of the protein. Antibodies to these repeats can neutralize sporozoite infectivity. Generation of protective antibody responses to the CS protein (anti-CS Ab) requires help by CD4 T cells. A CD4 T cell epitope from the CS protein designated T* was previously identified by screening T cells from volunteers immunized with irradiated P. falciparum sporozoites. The T* sequence spans twenty amino acids that contains multiple T cell epitopes restricted by various HLA alleles. Subunit malaria vaccines including T* are highly immunogenic in rodents, non-human primates and humans. In this study we characterized a highly conserved HLA-DRβ1*04:01 (DR4) restricted T cell epitope (QNT-5) located at the C-terminus of T*. We found that a peptide containing QNT-5 was able to elicit long-term anti-CS Ab responses and prime CD4 T cells in HLA-DR4 transgenic mice despite forming relatively unstable MHC-peptide complexes highly susceptible to HLA-DM editing. We attempted to improve the immunogenicity of QNT-5 by replacing the P1 anchor position with an optimal tyrosine residue. The modified peptide QNT-Y formed stable MHC-peptide complexes highly resistant to HLA-DM editing. Contrary to expectations, a linear peptide containing QNT-Y elicited almost 10-fold lower long-term antibody and IFN-γ responses compared to the linear peptide containing the wild type QNT-5 sequence. Some possibilities regarding why QNT-5 is more effective than QNT-Y in inducing long-term T cell and anti-CS Ab when used as vaccine are discussed.

Pertenece a

Directory of Open Access Journals - Articles  


Carlos A Parra -  López -  David Bernal -  Estévez -  Liusong Yin -  Luis Eduardo Vargas -  Carolina Pulido -  Calixto -  Luz Mary Salazar -  J Mauricio Calvo -  Calle -  Lawrence J Stern - 

Id.: 65669506

Idioma: EN  - 

Versión: 1.0

Estado: Final

Palabras claveMedicine - 

Tipo de recurso: article  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí


Requerimientos técnicos:  Browser: Any - 

Relación: [IsBasedOn] PLoS ONE, Vol 9, Iss 7, p e100639 (2014)
[References] http://europepmc.org/articles/PMC4077652?pdf=render
[References] https://doaj.org/toc/1932-6203

Fecha de contribución: 16-jun-2016


* 10.1371/journal.pone.0100639
* 1932-6203

Otros recursos del mismo autor(es)

  1. Derecho a la ciudad y una experiencia educativa popular En el presente artículo retomamos los avances de un proceso de investigación antropológica en el cam...
  2. A case of splenic myelolipoma in a German Shepherd dog Peer reviewed
  3. Study of the evolution of gravel beaches nourished with sand Coastal erosion is a worldwide problem, so accurate knowledge of the factors involved in the shoreli...
  4. Templated Synthesis of Pr-Doped Ceria with Improved Micro and Mesoporosity Porosity, Redox Properties and Catalytic Activity A Ce0.9Pr0.1O2 catalyst has been prepared using nitrates as metal precursors, which have been infilt...
  5. Multiple solutions and numerical analysis to the dynamic and stationary models coupling a delayed energy balance model involving latent heat and discontinuous albedo with a deep ocean We study a climatologically important interaction of two of the main components of the geophysical s...

Otros recursos de la mismacolección

No existen otros recursos

Aviso de cookies: Usamos cookies propias y de terceros para mejorar nuestros servicios, para análisis estadístico y para mostrarle publicidad. Si continua navegando consideramos que acepta su uso en los términos establecidos en la Política de cookies.