1) La descarga del recurso depende de la página de origen
2) Para poder descargar el recurso, es necesario ser usuario registrado en Universia

Opción 1: Descargar recurso

Opción 2: Descargar recurso

Detalles del recurso


We examined the inhibitory activity of angiotensin I converting enzyme (ACE) in protein hydrolysates from dulse, Palmaria palmata. The proteins extracted from dulse were mainly composed of phycoerythrin (PE) followed by phycocyanin (PC) and allophycocyanin (APC). The dulse proteins showed slight ACE inhibitory activity, whereas the inhibitory activity was extremely enhanced by thermolysin hydrolysis. The ACE inhibitory activity of hydrolysates was hardly affected by additional pepsin, trypsin and chymotrypsin treatments. Nine ACE inhibitory peptides (YRD, AGGEY, VYRT, VDHY, IKGHY, LKNPG, LDY, LRY, FEQDWAS) were isolated from the hydrolysates by reversed-phase high-performance liquid chromatography (HPLC), and it was demonstrated that the synthetic peptide LRY (IC50: 0.044 mol) has remarkably high ACE inhibitory activity. Then, we investigated the structural properties of dulse phycobiliproteins to discuss the origin of dulse ACE inhibitory peptides. Each dulse phycobiliprotein possesses -subunit (Mw: 17,477-17,638) and -subunit (Mw: 17,455-18,407). The sequences of YRD, AGGEY, VYRT, VDHY, LKNPG and LDY were detected in the primary structure of PE -subunit, and the LDY also exists in the APC - and -subunits. In addition, the LRY sequence was found in the -subunits of PE, PC and APC. From these results, it was suggested that the dulse ACE inhibitory peptides were derived from phycobiliproteins, especially PE. To make sure the deduction, we carried out additional experiment by using recombinant PE. We expressed the recombinant - and -subunits of PE (rPE and rPE, respectively), and then prepared their peptides by thermolysin hydrolysis. As a result, these peptides showed high ACE inhibitory activities (rPE: 94.4%; rPE: 87.0%). Therefore, we concluded that the original proteins of dulse ACE inhibitory peptides were phycobiliproteins.

Pertenece a

Hokkaido University Collection of Scholarly and Academic Papers  


Furuta, Tomoe -  Miyabe, Yoshikatsu -  Yasui, Hajime -  Kinoshita, Yasunori -  Kishimura, Hideki - 

Id.: 69985345

Idioma: inglés  - 

Versión: 1.0

Estado: Final

Palabras claveACE inhibitory activity - 

Tipo de recurso: article  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

: http://creativecommons.org/licenses/by/4.0/

Requerimientos técnicos:  Browser: Any - 

Fecha de contribución: 21-oct-2017


* Marine Drugs, 14(2): UNSP 32

Otros recursos del mismo autor(es)

  1. STRUCTURAL PROPERTIES OF PHYCOERYTHRIN FROM DULSE PALMARIA PALMATA We found that the red alga dulse (Palmaria palmata) contains a lot of proteins, which is mainly comp...
  2. Characteristics of Collagen from Rohu (Labeo rohita) Skin Acid soluble collagen (ASC) and pepsin soluble collagen (PSC) were isolated from rohu skin with the ...
  3. Characteristics of collagen from the skin of clown featherback (Chitala ornata) Acid soluble collagen (ASC) and pepsin soluble collagen (PSC) from the skin of clown featherback (Ch...
  4. Molecular characteristics and properties of gelatin from skin of seabass with different sizes Gelatin was extracted from the skin of seabass (Lutes calcarifer) with different average sizes (2, 4...
  5. Molecular and immunological characterization of -component (Onc k 5), a major IgE-binding protein in chum salmon roe Molecular characterization of a major allergen from salmon roe.Salmon roe has a high allergic potenc...

Otros recursos de la mismacolección

  1. Behavioral assay and chemical characters of female sex pheromones in the hermit crab Pagurus filholi Males of the hermit crab Pagurus filholi perform assessment behavior toward females, as a preliminar...
  2. Importance of patch size variation for the population persistence of a decapod crustacean in seagrass beds To understand the effects of patch attributes of seagrass beds on the persistence of an animal popul...
  3. An analysis of metabolic characteristics of planktonic heterotrophic protozoans Respiration rate data on 61 heterotrophic planktonic protozoans were compiled and analyzed as a func...
  4. Routine metabolic rates of pelagic marine fishes and cephalopods as a function of body mass, habitat temperature and habitat depth Cephalopods and fishes are major components of marine micronekton and nekton, so an understanding of...
  5. 深海性動物プランクトンの代謝活性(総説) Because of logistic difficulties and technical complexities, experimental study on the metabolism of...

Aviso de cookies: Usamos cookies propias y de terceros para mejorar nuestros servicios, para análisis estadístico y para mostrarle publicidad. Si continua navegando consideramos que acepta su uso en los términos establecidos en la Política de cookies.