1) La descarga del recurso depende de la página de origen
2) Para poder descargar el recurso, es necesario ser usuario registrado en Universia


Opción 1: Descargar recurso

Opción 2: Descargar recurso

Detalles del recurso

Descripción

All of the common techniques for obtaining structural information from macromolecular and supramolecular objects suffer from one type of drawback or another. X-ray and neutron diffraction methods have the phase problem (only the amplitude and not the phase angle of the complex diffraction vector can be experimentally observed). Low-dose 3-dimensional electron microscopy suffers from the inability to collect a complete data set due to instrumental limitations. Although light microscopy allows the sample to be kept under biological conditions (compare with standard electron microscopic techniques), the low resolution has limited its appeal to more qualitative aspects of ultrastructure analysis.

All of these limitations can be considered as a lack of information in the Fourier space domain. A generalized mathematical approach is presented where global real-space constraints are utilized to ameliorate this loss of information. In simple cases (one-dimensional analysis) it is possible to solve the phase problem, and in more complex cases (two- and three-dimensional analyses) the added real-space information is used to augment the experimentally derived data. The iterative Fourier refinement scheme was chosen as it represents the most versatile means for incorporating global knowledge concerning the real-space behavior of the object.

This approach was used to solve the three-dimensional x-ray structure of the snake polypeptide neurotoxin α-Bungarotoxin, the one dimensional transmembrane electron density profile. for acetylcholine receptor containing membranes, and to solve the missing cone problem of electron microscopic structure analysis.

Real-space constraints were also incorporated into an iterative deconvolution scheme used both for image processing and for the quantitative analysis of overlapping gel electrophoretogram peaks. None of this work would have been possible if the information supplied by real-space constraints had not been utilized . The approaches presented to these problems are general ones and should be applicable to other systems.


Pertenece a

CaltechTHESIS  

Autor(es)

Agard, David Andrew - 

Id.: 71041027

Versión: 1.0

Estado: Final

Tipo:  application/pdf - 

Tipo de recurso: Thesis  -  NonPeerReviewed  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

Formatos:  application/pdf - 

Requerimientos técnicos:  Browser: Any - 

Relación: [References] http://resolver.caltech.edu/CaltechTHESIS:03022018-140501638
[References] https://thesis.library.caltech.edu/10748/

Fecha de contribución: 29-mar-2018

Contacto:

Localización:

Otros recursos que te pueden interesar

  1. The use of mobile devices in the college classroom: project method and mobile technologies in higher education This study reports partial results from the research entitled "Project Method and Mobile Technologie...
  2. Short films prodution in the classroom context: cinematographic language reading and fruition Brazilian education has been facing a lot of challenges: one of them is the necessity of fostering t...
  3. Problem-Based Learning: A Teaching and Learning Model for Organization Theory The knowledge construction of a human being occurs by means of assimilation and accommodation mechan...
  4. “A boogie-woogie made out with a tambourine and a guitar.” Project based learning applied in brazilian vocational education context This article is based on an ethnographic work that aims to show how project based learning can be ap...
  5. Going international: the challenges for the Brazilian Federal Institutes of Education, Science and Technology The present study assumes that, in a globalized world, the view of English as an international langu...

Otros recursos de la mismacolección

  1. Some Electronic Properties of ZnO and SrTiO^3

    The surface barrier systems consisting of gold and palladium on both chemically prepared and clea...

  2. Hormonal Control of Protein Granule Accumulation in Fat Bodies of Drosophila Melanogaster Larvae

    It has been previously shown that large granules of protein accumulate in the larval fat body of ...

  3. Electrodynamics in a Strong Magnetic Field

    This thesis is a study of the electrodynamic phenomena which can occur in strong magnetic fields ...

  4. The Unsteady Cavity in Internal Flows

    The problems of the two-dimensional unsteady cavity in internal flow are treated and linear theor...

  5. Phylogeny of the Later Tertiary Equidae in the Light of New Pliocene Horses from Chihuahua, Mexico [major thesis]. Origin of the Pioneer Pyrophyllite Deposit, San Diego County, California [minor thesis]

    [Major Thesis]. Phylogeny of the Later Tertiary Equidae in the Light of New Pliocene Horses from ...

Aviso de cookies: Usamos cookies propias y de terceros para mejorar nuestros servicios, para análisis estadístico y para mostrarle publicidad. Si continua navegando consideramos que acepta su uso en los términos establecidos en la Política de cookies.