1) La descarga del recurso depende de la página de origen
2) Para poder descargar el recurso, es necesario ser usuario registrado en Universia

Opción 1: Descargar recurso

Detalles del recurso


Conditional expression of diphtheria toxin receptor (DTR) is widely used for tissue-specific ablation of cells. However, diphtheria toxin (DT) crosses the blood-brain barrier, which limits its utility for ablating peripheral cells using Cre drivers that are also expressed in the central nervous system (CNS). Here we report the development of a brain-sparing DT, termed BRAINSPAReDT, for tissue-specific genetic ablation of cells outside the CNS. We prevent blood-brain barrier passage of DT through PEGylation, which polarizes the molecule and increases its size. We validate BRAINSPAReDT with regional genetic sympathectomy: BRAINSPAReDT ablates peripheral but not central catecholaminergic neurons, thus avoiding the Parkinson-like phenotype associated with full dopaminergic depletion. Regional sympathectomy compromises adipose tissue thermogenesis, and renders mice susceptible to obesity. We provide a proof of principle that BRAINSPAReDT can be used for Cre/DTR tissue-specific ablation outside the brain using CNS drivers, while consolidating the link between adiposity and the sympathetic nervous system.

Pertenece a

ARCA - Access to Research and Communication Annals - IGC Repository  


Pereira, Mafalda M. A. -  Mahú, Inês -  Seixas, Elsa -  Martinéz -  Sánchez, Noelia -  Kubasova, Nadiya -  Pirzgalska, Roksana M -  Cohen, Paul -  Dietrich, Marcelo O -  López, Miguel -  Bernardes, Gonçalo J. L. -  Domingos, Ana I. - 

Id.: 69783636

Idioma: eng  - 

Versión: 1.0

Estado: Final

Palabras claveChemical modification - 

Tipo de recurso: article  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

: openAccess

Requerimientos técnicos:  Browser: Any - 

Relación: [References] http://www.nature.com/articles/ncomms14967

Fecha de contribución: 12-abr-2017


* Pereira, M. M. A. et al. A brain-sparing diphtheria toxin for chemical genetic ablation of peripheral cell lineages. Nat. Commun. 8, 14967 doi: 10.1038/ncomms14967 (2017).
* 10.1038/ncomms14967

Otros recursos del mismo autor(es)

  1. What should be evaluated from a manager's perspective? Developing a salient protected area effectiveness evaluation system for managers and scientists in Spain The System for the Integrated Assessment of Protected Areas (SIAPA) was developed as a tool to impro...
  2. Three decades of land-use changes in the region of Madrid and how they relate to territorial planning In recent decades, the region of Madrid has experienced extensive changes in land use-land cover (LU...
  3. The σ1 Receptor Engages the Redox-Regulated HINT1 Protein to Bring Opioid Analgesia Under NMDA Receptor Negative Control © Copyright 2015, Mary Ann Liebert, Inc. Aims: The in vivo pharmacology of the sigma 1 receptor (σ1R...
  4. Proposal of a system for the integrated and comparative assessment of protected areas Appendix A: Supplementary data http://dx.doi.org/10.1016/j.ecolind.2012.05.009
  5. Results of the implementation of the System for the Integrated Assessment of Protected Areas (SIAPA) to the protected areas of the Autonomous Region of Madrid (Spain) Supplementary data: http://dx.doi.org/10.1016/j.ecolind.2013.04.019

Otros recursos de la mismacolección

  1. Leptin Resistance and the Neuro-Adipose Connection This article was submitted to Cellular Endocrinology, a section of the journal Frontiers in Endocrin...
  2. Curcumin Inhibits Gastric Inflammation Induced by Helicobacter Pylori Infection in a Mouse Model Helicobacter pylori (H. pylori) infection triggers a sequence of gastric alterations starting with a...
  3. Hypothalamic melanin concentrating hormone neurons communicate the nutrient value of sugar Sugars that contain glucose, such as sucrose, are generally preferred to artificial sweeteners owing...
  4. The reward value of sucrose in leptin-deficient obese mice Leptin-deficient patients report higher "liking" ratings for food, and leptin replacement therapy no...

Aviso de cookies: Usamos cookies propias y de terceros para mejorar nuestros servicios, para análisis estadístico y para mostrarle publicidad. Si continua navegando consideramos que acepta su uso en los términos establecidos en la Política de cookies.