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1. Background and Objectives: Although classified as a micronutrient, plants accumulate chloride (Cl-) to levels that are typical of the content of a macronutrient. This enables plants to improve cell osmoregulation, water relations and growth under optimal growing conditions (Franco-Navarro et al., 2016). Under salt stress conditions Cl- impairs nitrate (NO3-) nutrition due, apparently, to competition for membrane transport mechanisms. Chloride loading into root xylem vessels is a key mechanism regulating shoot Cl- accumulation (Brumós et al., 2010). To maintain an appropriate balance between both anions, xylem loading of NO3- and Cl- has to be tightly regulated. Given the thermodynamics of anion transport, release of Cl- and NO3- into the root xylem is highly likely to be electrochemically passive and, therefore, facilitated by plasma membrane anion channels that are not yet identified. The NO3- and Cl- conductance of xylem parenchyma cells registered by early patch clamp studies are reminiscent to electrical characteristics of the recently identified anion channels from the slow-type (SLAC/SLAH) family, consisting of five members in Arabidopsis thaliana. To determine the involvement of the slow-type channels in plant Cl- homeostasis, a member of the gene family predominantly expressed in the root has been functionally characterized 2. Material and Methods: Tissue and cell-specific expression pattern of the gene was determined in different transgenic lines of Arabidopsis thaliana Col-0 expressing the chimeric GUS::GFP marker gene under the control of the native gene promoter. Gene expression in response to different abiotic stress and nutritional treatments was quantified by Quantitative Real Time-PCR (qPCR). Homozygous knock-out mutant plants were phenotyped according to plant growth, shoot Cl- content and xylem sap Cl- concentration. Electrophysiological activity of the gene product was characterized through functional expression of the gene in Xenopus laevis oocytes and Double-Electrode Voltage-Clamp (DEVC) measurements. 3. Results: The following results regarding the functional characterization of a gene encoding a slow-type root anion channel will be presented: cell-type expression, gene regulation, knockout mutant phenotypes, as well as electrophysiological and regulatory mechanisms involved in protein activity regulation. 4. Conclusions: A novel anion channel with a pivotal role in Cl- homeostasis in higher plants has been characterized.

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Digital.CSIC  

Autor(es)

Cubero Font, Paloma -  Espartero, Joaquín -  Maierhofer, Tobias -  Rosales Villegas, Miguel Á. -  Hedrich, Rainer -  Díaz -  Rueda, P. -  Geiger, Dietmar -  Colmenero Flores, José M. - 

Id.: 70835266

Idioma: eng  - 

Versión: 1.0

Estado: Final

Tipo de recurso: Comunicación de congreso  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

: closedAccess

Requerimientos técnicos:  Browser: Any - 

Relación: [References] MINECO/ICTI2013-2016/AGL2015-71386-R

Fecha de contribución: 17-ene-2018

Contacto:

Localización:
* XVI Simposio Hispano-Luso de Nutrición Mineral de las Plantas. San Pedro del Pinatar, Murcia, 25-28 de Septiembre de 2016

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