1) La descarga del recurso depende de la página de origen
2) Para poder descargar el recurso, es necesario ser usuario registrado en Universia


Opción 1: Descargar recurso

Opción 2: Descargar recurso

Detalles del recurso

Descripción

1. Background and Objectives: Although classified as a micronutrient, plants accumulate chloride (Cl-) to levels that are typical of the content of a macronutrient. This enables plants to improve cell osmoregulation, water relations and growth under optimal growing conditions (Franco-Navarro et al., 2016). Under salt stress conditions Cl- impairs nitrate (NO3-) nutrition due, apparently, to competition for membrane transport mechanisms. Chloride loading into root xylem vessels is a key mechanism regulating shoot Cl- accumulation (Brumós et al., 2010). To maintain an appropriate balance between both anions, xylem loading of NO3- and Cl- has to be tightly regulated. Given the thermodynamics of anion transport, release of Cl- and NO3- into the root xylem is highly likely to be electrochemically passive and, therefore, facilitated by plasma membrane anion channels that are not yet identified. The NO3- and Cl- conductance of xylem parenchyma cells registered by early patch clamp studies are reminiscent to electrical characteristics of the recently identified anion channels from the slow-type (SLAC/SLAH) family, consisting of five members in Arabidopsis thaliana. To determine the involvement of the slow-type channels in plant Cl- homeostasis, a member of the gene family predominantly expressed in the root has been functionally characterized 2. Material and Methods: Tissue and cell-specific expression pattern of the gene was determined in different transgenic lines of Arabidopsis thaliana Col-0 expressing the chimeric GUS::GFP marker gene under the control of the native gene promoter. Gene expression in response to different abiotic stress and nutritional treatments was quantified by Quantitative Real Time-PCR (qPCR). Homozygous knock-out mutant plants were phenotyped according to plant growth, shoot Cl- content and xylem sap Cl- concentration. Electrophysiological activity of the gene product was characterized through functional expression of the gene in Xenopus laevis oocytes and Double-Electrode Voltage-Clamp (DEVC) measurements. 3. Results: The following results regarding the functional characterization of a gene encoding a slow-type root anion channel will be presented: cell-type expression, gene regulation, knockout mutant phenotypes, as well as electrophysiological and regulatory mechanisms involved in protein activity regulation. 4. Conclusions: A novel anion channel with a pivotal role in Cl- homeostasis in higher plants has been characterized.

Pertenece a

Digital.CSIC  

Autor(es)

Cubero Font, Paloma -  Espartero, Joaquín -  Maierhofer, Tobias -  Rosales Villegas, Miguel Á. -  Hedrich, Rainer -  Díaz -  Rueda, P. -  Geiger, Dietmar -  Colmenero Flores, José M. - 

Id.: 70835266

Idioma: eng  - 

Versión: 1.0

Estado: Final

Tipo de recurso: Comunicación de congreso  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

: closedAccess

Requerimientos técnicos:  Browser: Any - 

Relación: [References] MINECO/ICTI2013-2016/AGL2015-71386-R

Fecha de contribución: 15-jun-2018

Contacto:

Localización:
* XVI Simposio Hispano-Luso de Nutrición Mineral de las Plantas. San Pedro del Pinatar, Murcia, 25-28 de Septiembre de 2016

Otros recursos del mismo autor(es)

  1. Cuidado sensible y seguridad del apego en preescolares Abstract: This study was based on sensitivity hypothesis of attachment theory that relates sensitive...
  2. Sistema de Tareas Diagnósticas y la habilidad lectora Abstract: The objective of this research is to determine the relationship between the System of Diag...
  3. El proceso de apropiación social del conocimiento en cooperativas de cacao del Estado Táchira, Venezuela This research aims to analyze the process of social appropriation of knowledge of the cocoa producer...
  4. La política alimentaria en Venezuela (1999-2015):: Origen y evolución Political, economic and social transformations since 1999 had influenced the orientation of public p...
  5. Extracción etanólica de aceite de semillas de Moringa oleifera Moringa oleifera is highly valued worldwide due to its properties. One of the profitable parts of th...

Otros recursos de la mismacolección

  1. Understanding mechanisms to predict and optimize biochar for agrochemical sorption The ability of biochars to bind various organic compounds has been widely studied due to the potenti...
  2. RNA-seq of plant/pathogen interactions as a tool for deciphering effector genes Trabajo presentado en la 2nd Annual Conference of the SUSTAIN Action, celebrada en Zakopane (Poland)...
  3. Identifying allelic variants for papilla based powdery mildew adult plant resistance against powdery mildew in oat by association studies Trabajo presentado en la 2nd Annual Conference of the SUSTAIN Action, celebrada en Zakopane (Poland)...
  4. A functional approach to test the response of weed communities to agriculture intensification across two countries Trabajo presentado en el 17th European Weed Research Society Symposium (EWRS), celebrado en Montpel...
  5. Genetic characterization of the interaction between sunflower and Orobanche cumana Trabajo presentado en el 13th World Congress on Parasitic Plants (Parasitic plants: the good, the ba...

Aviso de cookies: Usamos cookies propias y de terceros para mejorar nuestros servicios, para análisis estadístico y para mostrarle publicidad. Si continua navegando consideramos que acepta su uso en los términos establecidos en la Política de cookies.