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Rotifers are an important group of zooplankton in aquatic ecosystems that contain relatively high amounts of unsaturated fatty acids, proteins and peptides. Rotifers, especially Brachionus plicatilis species, are one of the important live food sources for marine fish larvae in aquaculture. The aim of this study was to evaluate the antioxidant activities of purified peptides from B. plicatilis. Antioxidant peptides of the B. plicatilis have been hydrolyzed by Alcalase, α¬Chymotrypsin, Papain, Neutrase, Pepsin, and Trypsin. Their antioxidant activity was evaluated by the free radical inhibitory effect of diphenylpicrylhydrazyl (DPPH). Also the sequential chromatography method was used for extraction and purification of the peptides. The results showed that peptides obtained from the pepsin hydrolysate have a higher inhibitory effect than other peptides. Pepsin showed 58% inhibitory effect in 0.015 mM DPPH. Finally, the pepsin hydrolysate constitutes were purified and isolated by gel-filtration chromatography (Sephadex G-250) and reverse-phase liquid chromatography on Eurospher C18 column (250×4.6 mm), respectively. The results of this study have been recognized the high antioxidant activities of extracted hydrolysate from B. plicatilis and their feasibility of using them in food industries as a food complement.

Pertenece a

Aquatic Commons  


Khafaeizadeh, K. -  Sakhaei, N. -  Doustshenas, B. -  Ghanemi, K. -  Zolgharnein, H. - 

Id.: 70982828

Idioma: fa  - 

Versión: 1.0

Estado: Final

Tipo:  application/pdf - 

Palabras claveBiology - 

Tipo de recurso: Article  -  PeerReviewed  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

Formatos:  application/pdf - 

Requerimientos técnicos:  Browser: Any - 

Relación: [References] http://aquaticcommons.org/22115/

Fecha de contribución: 10-feb-2018


* Khafaeizadeh, K. and Sakhaei, N. and Doustshenas, B. and Ghanemi, K. and Zolgharnein, H. (2016) Evaluation of antioxidant activity of the purified peptides from hydrolysis of rotifer (Brachionus plicatilis). Iranian Scientific Fisheries Journal, 25(2), pp. 69-78.

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