1) La descarga del recurso depende de la página de origen
2) Para poder descargar el recurso, es necesario ser usuario registrado en Universia


Opción 1: Descargar recurso

Detalles del recurso

Descripción

In order to determine the suitability of reference or housekeeping genes as internal controls in real-time reverse transcriptase PCR (RT-PCR) assays for quantification of target mRNAs, we studied the levels of expression of four candidate reference genes in maritime pine by real-time RT-PCR. The expression levels obtained for glyceraldehyde-3-phosphate-dehydrogenase, 18S ribosomal RNA, eukaryotic translation initiation factor eIF4AII and ubiquitin in nine stages of embryo development revealed that none of the genes tested proved to be suitable as an internal control. Copy number quantification of the four transcripts showed an average relative variation of seven fold. We propose that the combination of a precise method for RNA quantification, internal controls for monitoring RT reaction and PCR efficiency and a robust external standard curve can guarantee a reliable absolute quantification of mRNA transcripts in real time RT-PCR. This approach may avoid the controversy in the use of housekeeping genes and may assume special significance in tissues undergoing developmental changes.

Pertenece a

Repositório do ISPA Instituto Superior de Psicologia Aplicada  

Autor(es)

Gonçalves, Sónia -  Cairney, John -  Maroco, João -  Oliveira, Maria Margarida -  Miguel, Célia M. - 

Id.: 55782621

Idioma: eng  - 

Versión: 1.0

Estado: Final

Palabras claveHousekeeping genes - 

Tipo de recurso: article  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

: restrictedAccess

Requerimientos técnicos:  Browser: Any - 

Fecha de contribución: 24-sep-2012

Contacto:

Localización:
* Planta, 222, 556-563
* 0032-0935

Otros recursos del mismo autor(es)

  1. Psychometric properties of the World Health Organization Quality of Life Instrument-Abbreviated version in Portuguese-speaking adults from three different countries To evaluate the validity, reliability and invariance of the World Health Organization Quality of Lif...
  2. Cross-cultural validation of the brazilian portuguese version of the pain vigilance and awareness questionnaire Aims: To cross-culturally adapt the Pain Vigilance and Awareness Questionnaire (PVAQ) to the Brazili...
  3. Impact of inherent aspects of body image, eating behavior and perceived health competence on quality of life of university students The aim of this study was to assess the impact of inherent aspects of body image, eating behavior an...
  4. Time perception in mild cognitive impairment: interval length and subjective passage of time Objectives Patients with mild cognitive impairment (MCI) may have difficulties in time perception, w...
  5. A brief measure of narcissism among female juvenile delinquents and community youths: The narcissistic personality inventory–13 Research on narcissism has a long tradition, but there is limited knowledge regarding its applicatio...

Otros recursos de la mismacolección

  1. Accounting for animal density gradients using independent information in distance sampling surveys Distance sampling is extensively used for estimating animal density or abundance. Conventional metho...
  2. New allelic variants found in key rice salt-tolerance genes an association study Salt stress is a complex physiological trait affecting plants by limiting growth and productivity. R...
  3. Drought stress response in Jatropha curcas: Growth and physiology Tolerance to drought remains poorly described for Jatropha curcas accessions from different geograph...
  4. Modeling carcass removal time for avian mortality assessment in wind farms using survival analysis In monitoring studies at wind farms, the estimation of bird and bat mortality caused by collision mu...
  5. Effects of partial root-zone drying irrigation on cluster microclimate and fruit composition of field-grown Castelão grapevines The partial root-zone drying (PRD) irrigation technique has been proposed for viticulture as a possi...

Aviso de cookies: Usamos cookies propias y de terceros para mejorar nuestros servicios, para análisis estadístico y para mostrarle publicidad. Si continua navegando consideramos que acepta su uso en los términos establecidos en la Política de cookies.