1) La descarga del recurso depende de la página de origen
2) Para poder descargar el recurso, es necesario ser usuario registrado en Universia

Opción 1: Descargar recurso

Detalles del recurso


Glucagon like peptide-1 (GLP-1) is released from intestinal L-cells in response to nutrient ingestion and acts upon pancreatic β-cells potentiating glucose-stimulated insulin secretion and stimulating β-cell proliferation, differentiation, survival and gene transcription. These effects are mediated through the activation of multiple signal transduction pathways including the extracellular regulated kinase (ERK) pathway. We have previously reported that GLP-1 activates ERK through a mechanism dependent upon the influx of extracellular Ca2+ through L-type voltage gated Ca2+ channels (VGCC). However, the mechanism by which L-type VGCCs couple to the ERK signalling pathway in pancreatic β-cells is poorly understood. In this report, we characterise the relationship between L-type VGCC mediated changes in intracellular Ca2+ concentration ([Ca2+]i) and the activation of ERK, and demonstrate that the sustained activation of ERK (up to 30 min) in response to GLP-1 requires the continual activation of the L-type VGCC yet does not require a sustained increase in global [Ca2+]i or Ca2+ efflux from the endoplasmic reticulum. Moreover, sustained elevation of [Ca2+]i induced by ionomycin is insufficient to stimulate the prolonged activation of ERK. Using the cell permeant Ca2+ chelators, EGTA-AM and BAPTA-AM, to determine the spatial dynamics of L-type VGCC-dependent Ca2+ signalling to ERK, we provide evidence that a sustained increase in Ca2+ within the microdomain of the L-type VGCC is sufficient for signalling to ERK and that this plays an important role in GLP-1- stimulated ERK activation.

Pertenece a

Faculty of Technology ePrints Service  


Selway, J. -  Rigatti, R. -  Storey, N. -  Lu, J. -  Willars, G. B. -  Herbert, T. P. - 

Id.: 70138750

Idioma: inglés  - 

Versión: 1.0

Estado: Final

Tipo:  application/pdf - 

Palabras claveB200 Pharmacology, Toxicology and Pharmacy - 

Tipo de recurso: Article  -  PeerReviewed  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

: cc_by4

Formatos:  application/pdf - 

Requerimientos técnicos:  Browser: Any - 

Relación: [References] http://eprints.lincoln.ac.uk/28223/
[References] https://doi.org/10.1371/journal.pone.0033004
[References] 10.1371/journal.pone.0033004

Fecha de contribución: 18-oct-2017


* Selway, J. and Rigatti, R. and Storey, N. and Lu, J. and Willars, G. B. and Herbert, T. P. (2012) Evidence that Ca 2+ within the microdomain of the l-type voltage gated Ca 2+ channel activates ERK in MIN6 cells in response to glucagon-like peptide-1. PLoS ONE, 7 (3). 0033004. ISSN 1932-6203

Otros recursos del mismo autor(es)

  1. Ground layer adaptive optics for the W. M. Keck Observatory: feasibility study Ground-layer adaptive optics (GLAO) systems offer the possibility of improving the ”seeing” of large...
  2. Measurement of b hadron lifetimes in pp collisions at √s = 8 TeV Measurements are presented of the lifetimes of the B[superscript 0], B[superscript 0][subscript s], ...
  3. Measurements of differential cross sections of top quark pair production as a function of kinematic event variables in proton-proton collisions at √s=13 TeV Measurements of differential t[¯ over t] production cross sections are presented in the single-lepto...
  4. Inclusive Search for a Highly Boosted Higgs Boson Decaying to a Bottom Quark-Antiquark Pair An inclusive search for the standard model Higgs boson (H) produced with large transverse momentum (...
  5. Synthesis process and thermoelectric properties of n-type tin selenide thin films International audience

Otros recursos de la mismacolección

  1. Structural complexity of the co-chaperone SGTA: a conserved C-terminal region is implicated in dimerization and substrate quality control Protein quality control mechanisms are essential for cell health and involve delivery of proteins to...
  2. “We aren’t idlers”: Using subjective group dynamics to promote prosocial driver behavior at long-wait stops Idling engines are a substantial air pollutant which contribute to many health and environmental pro...
  3. Commentary on: “ Implementing a Mediterranean-Style Diet Outside the Mediterranean Region ” Letter to the editor of the journal
  4. Does an aging population influence stock markets? Evidence from New Zealand The effect of the aging baby-boom-cohort on asset values is extensively studied. While that effect v...
  5. From Economic to Symbiotic Constitutionalism: a Belated Post-Lisbon, Post-Crisis Transformation? This paper departs from the traditional portrayal of the relationship between economic and social co...

Aviso de cookies: Usamos cookies propias y de terceros para mejorar nuestros servicios, para análisis estadístico y para mostrarle publicidad. Si continua navegando consideramos que acepta su uso en los términos establecidos en la Política de cookies.