1) La descarga del recurso depende de la página de origen
2) Para poder descargar el recurso, es necesario ser usuario registrado en Universia

Opción 1: Descargar recurso

Detalles del recurso


Chapter One

Bis(methidium)spermine (BMSp), a dimer of two intercalating monomers of ethidium bromide (EB) connected by a spermine link has been synthesized and characterized. The results of these studies clearly demonstrate that both monomers of BMSp simultaneously intercalate nucleic acid, substantially enhancing both its binding affinity and specificity. Under physiological conditions both the binding affinity and specificity of BMSp are similar to DNA binding regulatory proteins. Thus BMSp represents one of the first rationally designed drugs which may selectively inhibit or alter gene expression.

The binding affinity, binding cooperativity, binding site size, and visible spectrum of BMSp are found to vary with nucleic acid conformation. Both BMSp and EB are shown to bind H and A conformations of nucleic acid much more tightly than B conformations. As a result, both compounds induce sequence specific B → H → A allosteric transitions in DNA.

Chapter Two

A sensitive experimental technique which can accurately estimate equilibrium binding isotherms is described. Ligand-macromolecule interactions are monitored by classical indirect techniques over a broad ratio of LT/M, where LT is the total ligand concentration and M is the macromolecule concentration. When analyzed at constant X, where X is some physical property of ligand which is proportional to its concentration, the dependence of LT on M can be used to estimate the binding densities and free ligand concentrations characterizing the ligand-macromolecule interaction. In contrast to classical indirect and direct techniques, accurate binding isotherms can be estimated over a wide range of binding densities for tightly or weakly bound ligands. When the binding of ethidium bromide to polyd(C-G) and polydCdG is examined by this technique, previously undetected allosteric transitions are revealed.

Chapter Three

Evidence for a new conformational family of Watson-Crick DNA is presented. Termed H or hybrid DNA, such DNA is postulated to be an intermediate in the interfamily B → A transition. Hybrid DNA is characterized by a 2'-endo (3' → 5') 3'-endo alteration in sugar pucker every base pair and may also be an intermediate in DNA melting, DNA kinking, and drug intercalation. The ease with which DNA undergoes a B → H → A transition is found to vary greatly with its sequence. On the basis of these results, the equilibrium stability, rather than the structure of Watson-Crick DNA is postulated to vary greatly with base sequence.

Pertenece a



Becker, Michael McClellan - 

Id.: 71304328

Versión: 1.0

Estado: Final

Tipo:  application/pdf - 

Tipo de recurso: Thesis  -  NonPeerReviewed  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

Formatos:  application/pdf - 

Requerimientos técnicos:  Browser: Any - 

Relación: [References] http://resolver.caltech.edu/CaltechTHESIS:05102018-162757284
[References] https://thesis.library.caltech.edu/10874/

Fecha de contribución: 12-may-2018


* Becker, Michael McClellan (1982) I. Molecular Recognition of Nucleic Acid by BMSP. II. Sequence Specific B → H → A Allosteric Transitions in DNA. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/s7mc-ka23. http://resolver.caltech.edu/CaltechTHESIS:05102018-162757284

Otros recursos que te pueden interesar

  1. Effects of spices on the chemical properties of hot-smoked catfish (Clarias gariepinus) Effect of spices on the chemical properties of hot-smoked catfish (C.gariepinus) was examined. The e...
  2. Evaluation of beach seine net by-catch in Lake Kainji, Nigeria Studies on the evaluation of Beach Seine net by-catch in Lake Kainji was conducted in Yuna fishing v...
  3. Effect of mesh size on catch performance of driftnets fishing in Lake Kainji The studies on effect of mesh size on catch performance of driftnets fishing in Lake Kainji was expe...
  4. Survey of the present and fast disappearing fish species along two rivers in the Niger Delta Fisheries resources are on the decline in Nigeria due to over exploitation and inadequate management...
  5. Assessment of fishing gear and crafts utilized by fishermen in Eleyele Lake, Ibadan, Oyo State The study was conducted to assess the evolution of fishing gears and crafts utilized by fishermen in...

Otros recursos de la mismacolección

  1. Essays on Early-stage Financing and Firm Behavior

    The first chapter of this thesis studies the role of angel finance in the early-stage capital mar...

  2. Shear Wave Vibrational Directions and Related Fault Movements in Southern California Earthquakes [major thesis]. The Relationship of the Modelo and Ridge Route Formations in the Southern Ridge Basin, California [minor thesis]

    Shear wave vibrational directions and related fault movements in southern California earthquakes:...

  3. Search for SUSY with Delayed Photons at the Compact Muon Solenoid The Compact Muon Solenoid (CMS) experiment records data from proton-proton (pp) and heavy ion (Pb-Pb...
  4. Definability and Classification of Equivalence Relations and Logical Theories

    This thesis consists of four independent papers.

    In the first paper, joint with Kechris, ...

  5. An LES and RANS Study of the Canonical Shock-Turbulence Interaction

    The canonical problem of a nearly stationary, nearly planar shockwave passing through isotropic t...

Aviso de cookies: Usamos cookies propias y de terceros para mejorar nuestros servicios, para análisis estadístico y para mostrarle publicidad. Si continua navegando consideramos que acepta su uso en los términos establecidos en la Política de cookies.