1) La descarga del recurso depende de la página de origen
2) Para poder descargar el recurso, es necesario ser usuario registrado en Universia

Opción 1: Descargar recurso

Opción 2: Descargar recurso

Detalles del recurso


Microalgae are an outstanding source of high value products with applications in food, feed or biofuel production. Among these promising organisms, the haptophyte Tisochrysis lutea is commonly used as a feed for shellfish and shrimps in aquaculture. Its capacity to produce high amounts of docosahexanoic acid and storage lipids is also of economic interest for nutrition and energy production. Consequently, understanding its lipid accumulation under nitrogen deprivation is of major interest. Here, we aimed to identify Transcription Factors (TFs) involved in the establishment of the mutant phenotype of the 2Xc1 strain of T. lutea, which accumulates double the quantity of storage lipids under nitrogen deprivation than the wild type strain (WTc1). Strains were grown in chemostats and subjected to different nitrogen availability (limitation, repletion and depletion). Using RNA-seq data, the differentially expressed genes (DEGs) between strains were identified and summarized as a co-expression network to pinpoint putative major TFs in mutant phenotypes. This analysis was followed by a complementary Weighted Gene Correlation Network Analysis in order to classify genes based on their relative importance to mutant phenotype features, regardless of annotation biases due to the lack of functional annotation of the Tisochrysis lutea draft genome. This network-like strategy allowed the identification of seven TF candidates related to key functions in the mutant strain compared with WTc1. In particular, MYB-2R_14 and NF-YB_2 TFs are related to photosynthesis, oxidative stress response and triacylglycerol synthesis. GATA_2, MYB-rel_11 and MYB-2R_20 TFs are likely to be related to nitrogen uptake or carbon and nitrogen recycling, feeding carbohydrate synthesis in the form of chrysolaminarin. Finally, a q-RT-PCR approach further characterized the role of MYB-rel_11 and MYB-2R_20, revealing an expression pattern dependent on nitrogen availability.

Pertenece a

ArchiMer, Institutional Archive of Ifremer (French Research Institute for Exploitation of the Sea)  


Thiriet -  rupert, Stanislas -  Carrier, Gregory -  Trottier, Camille -  Eveillard, D. -  Schoefs, B. -  Bougaran, Gael -  Cadoret, Jean -  paul -  Chenais, B. -  Saint -  jean, Bruno - 

Id.: 70872451

Idioma: eng  - 

Versión: 1.0

Estado: Final

Tipo:  application/pdf - 

Palabras claveAlgae - 

Tipo de recurso: Texto Narrativo  -  Publication  -  info:eu-repo/semantics/article  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

: 2018 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/).

Formatos:  application/pdf - 

Requerimientos técnicos:  Browser: Any - 

Relación: [IsBasedOn] Algal Research-biomass Biofuels And Bioproducts (2211-9264) (Elsevier Science Bv), 2018-03 , Vol. 30 , P. 59-72

Fecha de contribución: 10-mar-2018


* DOI:10.1016/j.algal.2017.12.011

Otros recursos de la mismacolección

  1. A Review of the Stable Isotope Bio-geochemistry of the Global Silicon Cycle and Its Associated Trace Elements Silicon (Si) is the second most abundant element in the Earth's crust and is an important nutrient i...
  2. δ11B as monitor of calcification site pH in divergent marine calcifying organisms The boron isotope composition (δ11B) of marine biogenic carbonates has been predominantly studied as...
  3. Larval rearing, nursery growing and implantation at oyster parks of the Argentinian oyster, Ostrea puelchana d'Orb The present work has been done in the frame of the mutual interest of both counlries concerning the ...
  4. Focus : Marine Nature Park of Mayotte, a case study
  5. Reproductive behaviour of two tilapia species ( Oreochromis niloticus , Linné, 1758; Sarotherodon melanotheron, Rüppel, 1852) in freshwater intra and interspecific pairing context Nile tilapia Oreochromis niloticus (NT) and Black-chinned tilapia Sarotherodon melanotheron (BCT) ar...

Aviso de cookies: Usamos cookies propias y de terceros para mejorar nuestros servicios, para análisis estadístico y para mostrarle publicidad. Si continua navegando consideramos que acepta su uso en los términos establecidos en la Política de cookies.