1) La descarga del recurso depende de la página de origen
2) Para poder descargar el recurso, es necesario ser usuario registrado en Universia


Opción 1: Descargar recurso

Opción 2: Descargar recurso

Detalles del recurso

Descripción

This study aims to evaluate the in vitro cytotoxic and anti-migratory effects of Ficus deltoidea L. on prostate cancer cells, identify the active compound/s and characterize their mechanism of actions. Two farmed varieties were studied, var. angustifolia (FD1) and var. deltoidea (FD2). Their crude methanolic extracts were partitioned into n-hexane (FD1h, FD2h) chloroform (FD1c, FD2c) and aqueous extracts (FD1a, FD2a). Antiproliferative fractions (IC50 < 30 μg/mL, SRB staining of PC3 cells) were further fractionated. Active compound/s were dereplicated using spectroscopic methods. In vitro mechanistic studies on PC3 and/or LNCaP cells included: annexin V-FITC staining, MMP depolarization measurements, activity of caspases 3 and 7, nuclear DNA fragmentation and cell cycle analysis, modulation of Bax, Bcl-2, Smac/Diablo, and Alox-5 mRNA gene expression by RT-PCR. Effects of cytotoxic fractions on 2D migration and 3D invasion were tested by exclusion assays and modified Boyden chamber, respectively. Their mechanisms of action on these tests were further studied by measuring the expression VEGF-A, CXCR4, and CXCL12 in PC3 cells by RT-PCR. FD1c and FD2c extracts induced cell death (P < 0.05) via apoptosis as evidenced by nuclear DNA fragmentation. This was accompanied by an increase in MMP depolarization (P < 0.05), activation of caspases 3 and 7 (P < 0.05) in both PC3 and LNCaP cell lines. All active plant extracts up-regulated Bax and Smac/DIABLO, down-regulated Bcl-2 (P < 0.05). Both FD1c and FD2c were not cytotoxic against normal human fibroblast cells (HDFa) at the tested concentrations. Both plant extracts inhibited both migration and invasion of PC3 cells (P < 0.05). These effects were accompanied by down-regulation of both VEGF-A and CXCL-12 gene expressions (P < 0.001). LC–MS dereplication using taxonomy filters and molecular networking databases identified isovitexin in FD1c; and oleanolic acid, moretenol, betulin, lupenone, and lupeol in FD2c. In conclusion, FD1c and FD2c were able to overcome three main hallmarks of cancer in PC3 cells: (1) apoptosis by activating of the intrinsic pathway, (2) inhibition of both migration and invasion by modulating the CXCL12-CXCR4 axis, and (3) inhibiting angiogenesis by modulating VEGF-A expression. Moreover, isovitexin is here reported for the first time as an antiproliferative principle (IC50 = 43 μg/mL, SRB staining of PC3 cells).

Pertenece a

IOE EPrints  

Autor(es)

Hanafi, MMM -  Afzan, A -  Yaakob, H -  Aziz, R -  Sarmidi, MR -  Wolfender, J -  L -  Prieto, JM - 

Id.: 70848512

Idioma: eng  - 

Versión: 1.0

Estado: Final

Tipo:  text - 

Palabras claveScience & Technology, Life Sciences & Biomedicine, Pharmacology & Pharmacy, Apoptosis, Migration, Invasion, PC3, LNCaP, Prostate Cancer, Ficus Deltoidea, Promotes Apoptosis, Aerial Roots, Microcarpa, Smac, 5 -  Lipoxygenase, Proliferation, Identification, Antioxidant, Triterpenes, Smac/Diablo - 

Tipo de recurso: Article  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

: open

Formatos:  text - 

Requerimientos técnicos:  Browser: Any - 

Relación: [IsBasedOn] Frontiers in Pharmacology , 8 , Article 895. (2017)

Fecha de contribución: 10-ene-2018

Contacto:

Localización:

Otros recursos del mismo autor(es)

  1. Influencia de residuos de carbón activado en la evolución térmica de morteros eco-eficientes PRE-CONPAT 16 España, “Avances en control de calidad, patología y recuperación de la construcción”; ...
  2. The Beginning of High Mountain Occupations in the Pyrenees. Human Settlements and Mobility from 18,000 cal BC to 2000 cal BC During the last two decades, the archaeological research carried out in the Pyrenees challenged the ...
  3. Development of quaternary calcrete in the Tensift Al Haouz area, Central Peer reviewed
  4. Modified mineral phases during clay ceramic firing Peer reviewed
  5. Holocene elemental, lead isotope and charcoal record from peat in Southern Poland Peer reviewed

Otros recursos de la mismacolección

  1. Cannabis Use and Hypomania in Young People: A Prospective Analysis BACKGROUND: Cannabis use in young people is common and associated with psychiatric disorders. Howeve...
  2. Internet-accessed sexually transmitted infection (e-STI) testing and results service: A randomised, single-blind, controlled trial BACKGROUND: Internet-accessed sexually transmitted infection testing (e-STI testing) is increasingly...
  3. A Bioengineered Three-Dimensional Cell Culture Platform Integrated with Microfluidics To Address Antimicrobial Resistance in Tuberculosis Antimicrobial resistance presents one of the most significant threats to human health, with the emer...
  4. Influence of feed/inoculum ratios and waste cooking oil content on the mesophilic anaerobic digestion of food waste Information on the anaerobic digestion (AD) of food waste (FW) with different waste cooking oil cont...
  5. Human trafficking for labour exploitation: the results of a two-phase systematic review mapping the European evidence base and synthesising key scientific research evidence

Aviso de cookies: Usamos cookies propias y de terceros para mejorar nuestros servicios, para análisis estadístico y para mostrarle publicidad. Si continua navegando consideramos que acepta su uso en los términos establecidos en la Política de cookies.