1) La descarga del recurso depende de la página de origen
2) Para poder descargar el recurso, es necesario ser usuario registrado en Universia


Detalles del recurso

Descripción

Multi-day tracking of cells in culture systems can provide valuable information in bioscience experiments. We report the development of a cell culture imaging system, named EmSight, which incorporates multiple compact Fourier ptychographic microscopes with a standard multiwell imaging plate. The system is housed in an incubator and presently incorporates six microscopes, imaging an ANSI standard 6-well plate at the same time. By using the same low magnification objective lenses (NA of 0.1) as the objective and the tube lens, the EmSight is configured as a 1:1 imaging system that, providing large field-of-view (FOV) imaging (5.7 mm × 4.3 mm) onto a low-cost CMOS imaging sensor. The EmSight improves the image resolution by capturing a series of images of the sample at varying illumination angles; the instrument reconstructs a higher-resolution image by using the iterative Fourier ptychographic algorithm. In addition to providing high-resolution brightfield and phase imaging, the EmSight is also capable of fluorescence imaging at the native resolution of the objectives. We characterized the system using a phase Siemens star target, and show four-fold improved coherent resolution (synthetic NA of 0.42) and a depth of field of 0.2 mm. To conduct live, long-term dopaminergic neuron imaging, we cultured ventral midbrain from mice driving eGFP from the tyrosine hydroxylase promoter. The EmSight system tracks movements of dopaminergic neurons over a 21 day period.

Pertenece a

Caltech Authors  

Autor(es)

Kim, Jinho -  Henley, Beverley M. -  Kim, Charlene H. -  Lester, Henry A. -  Yang, Changhuei - 

Id.: 70070189

Versión: 1.0

Estado: Final

Tipo de recurso: Book Section  -  PeerReviewed  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

Requerimientos técnicos:  Browser: Any - 

Relación: [References] http://resolver.caltech.edu/CaltechAUTHORS:20170831-081004023
[References] http://authors.library.caltech.edu/81011/

Fecha de contribución: 01-sep-2017

Contacto:

Localización:
* Kim, Jinho and Henley, Beverley M. and Kim, Charlene H. and Lester, Henry A. and Yang, Changhuei (2017) Incubator embedded cell culture imaging system (EmSight) based on Fourier ptychographic microscopy. In: Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV. Proceedings of SPIE. No.10068. Society of Photo-Optical Instrumentation Engineers , Bellingham, WA, Art. No. 100680X. ISBN 9781510605770. http://resolver.caltech.edu/CaltechAUTHORS:20170831-081004023

Otros recursos del mismo autor(es)

  1. In vivo study of optical speckle decorrelation time across depths in the mouse brain The strong optical scattering of biological tissue confounds our ability to focus light deeply into ...
  2. Focusing light through scattering media by transmission matrix inversion Focusing light through scattering media has broad applications in optical imaging, manipulation and ...
  3. Acute Ethanol Administration Upregulates Synaptic α4-Subunit of Neuronal Nicotinic Acetylcholine Receptors within the Nucleus Accumbens and Amygdala Alcohol and nicotine are two of the most frequently abused drugs, with their comorbidity well descri...
  4. TC299423, a Novel Agonist for Nicotinic Acetylcholine Receptors (E)-5-(Pyrimidin-5-yl)-1,2,3,4,7,8-hexahydroazocine (TC299423) is a novel agonist for nicotinic acet...
  5. Nicotine in the Endoplasmic Reticulum Nicotine activates plasma membrane (PM) nicotinic receptors (nAChRs), but also permeates into the en...

Otros recursos de la mismacolección

  1. The existence of efficient and incentive compatible equilibria with public goods In our previous paper, "Optimal Allocation of Public Goods...," (1977) we presented a mechanism for ...
  2. Aggregate expected consumer surplus as a welfare index with an application to price stabilization This paper presents necessary and sufficient conditions for the expected value of consumer surplus t...
  3. Transitive permutation groups and equipotent voting rules Let F a two-alternative voting rule and G_F the subgroup of permutations of the voters under which F...
  4. Single-cell multiplexed cytokine profiling of CD19 CAR-T cells reveals a diverse landscape of polyfunctional antigen-specific response Background: It remains challenging to characterize the functional attributes of chimeric antigen rec...
  5. Self-consistent Bulge/Disk/Halo Galaxy Dynamical Modeling Using Integral Field Kinematics We introduce a method for modeling disk galaxies designed to take full advantage of data from integr...

Aviso de cookies: Usamos cookies propias y de terceros para mejorar nuestros servicios, para análisis estadístico y para mostrarle publicidad. Si continua navegando consideramos que acepta su uso en los términos establecidos en la Política de cookies.