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Descripción

Multi-day tracking of cells in culture systems can provide valuable information in bioscience experiments. We report the development of a cell culture imaging system, named EmSight, which incorporates multiple compact Fourier ptychographic microscopes with a standard multiwell imaging plate. The system is housed in an incubator and presently incorporates six microscopes, imaging an ANSI standard 6-well plate at the same time. By using the same low magnification objective lenses (NA of 0.1) as the objective and the tube lens, the EmSight is configured as a 1:1 imaging system that, providing large field-of-view (FOV) imaging (5.7 mm × 4.3 mm) onto a low-cost CMOS imaging sensor. The EmSight improves the image resolution by capturing a series of images of the sample at varying illumination angles; the instrument reconstructs a higher-resolution image by using the iterative Fourier ptychographic algorithm. In addition to providing high-resolution brightfield and phase imaging, the EmSight is also capable of fluorescence imaging at the native resolution of the objectives. We characterized the system using a phase Siemens star target, and show four-fold improved coherent resolution (synthetic NA of 0.42) and a depth of field of 0.2 mm. To conduct live, long-term dopaminergic neuron imaging, we cultured ventral midbrain from mice driving eGFP from the tyrosine hydroxylase promoter. The EmSight system tracks movements of dopaminergic neurons over a 21 day period.

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Caltech Authors  

Autor(es)

Kim, Jinho -  Henley, Beverley M. -  Kim, Charlene H. -  Lester, Henry A. -  Yang, Changhuei - 

Id.: 70070189

Versión: 1.0

Estado: Final

Tipo de recurso: Book Section  -  PeerReviewed  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

Requerimientos técnicos:  Browser: Any - 

Relación: [References] http://resolver.caltech.edu/CaltechAUTHORS:20170831-081004023
[References] http://authors.library.caltech.edu/81011/

Fecha de contribución: 01-sep-2017

Contacto:

Localización:
* Kim, Jinho and Henley, Beverley M. and Kim, Charlene H. and Lester, Henry A. and Yang, Changhuei (2017) Incubator embedded cell culture imaging system (EmSight) based on Fourier ptychographic microscopy. In: Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV. Proceedings of SPIE. No.10068. Society of Photo-Optical Instrumentation Engineers , Bellingham, WA, Art. No. 100680X. ISBN 9781510605770. http://resolver.caltech.edu/CaltechAUTHORS:20170831-081004023

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