1) La descarga del recurso depende de la página de origen
2) Para poder descargar el recurso, es necesario ser usuario registrado en Universia

Opción 1: Descargar recurso

Detalles del recurso


Translation of the hepatitis C virus (HCV) RNA is mediated by the interaction of ribosomes and cellular proteins with an internal ribosome entry site (IRES) located within the 5â?²-untranslated region (5â?²-UTR). We have investigated whether small RNA molecules corresponding to the different stemâ??loop (SL) domains of the HCV IRES, when introduced in trans, can bind to the cellular proteins and antagonize their binding to the viral IRES, thereby inhibiting HCV IRES-mediated translation. We have found that a RNA molecule corresponding to SL III could efficiently inhibit HCV IRES-mediated translation in a dose-dependent manner without affecting cap-dependent translation. The SL III RNA was found to bind to most of the cellular proteins which interacted with the HCV 5â?²-UTR. A smaller RNA corresponding to SL e+f of domain III also strongly and selectively inhibited HCV IRES-mediated translation. This RNA molecule interacted with the ribosomal S5 protein and prevented the recruitment of the 40S ribosomal subunit. This study reveals valuable insights into the role of the SL structures of the HCV IRES in mediating ribosome entry. Finally, these results provide a basis for developing anti-HCV therapy using small RNA molecules mimicking the SL structures of the 5â?²-UTR to specifically block viral RNA translation.

Pertenece a

PubMed Central (PMC3 - NLM DTD)  


Ray, Partho Sarothi -  Das, Saumitra - 

Id.: 17955127

Idioma: inglés  - 

Versión: 1.0

Estado: Final

Palabras claveArticles - 

Tipo de recurso: Text  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

: Copyright © 2004 Oxford University Press

Requerimientos técnicos:  Browser: Any - 

Fecha de contribución: 16-feb-2008



Otros recursos del mismo autor(es)

  1. Influence of metabolic stress on translation of p53 isoforms p53 and its isoforms are integral in modulating transcriptional gene expression programs and maintai...
  2. Structural and molecular basis of interaction of HCV non-structural protein 5A with human casein kinase 1α and PKR



    Interaction of non-structural protein 5A (NS5A) of Hepatitis C ...

  3. Re-analysis of cryoEM data on HCV IRES bound to 40S subunit of human ribosome integrated with recent structural information suggests new contact regions between ribosomal proteins and HCV RNA In this study, we combine available high resolution structural information on eukaryotic ribosomes w...
  4. WWC1 genotype modulates age related decline in episodic memory function across the adult lifespan
  5. The beta hairpin structure within ribosomal protein S5 mediates interplay between domains II and IV and regulates HCV IRES function Translation initiation in Hepatitis C Virus (HCV) is mediated by Internal Ribosome Entry Site (IRES)...

Otros recursos de la misma colección

  1. Structure-Specific nuclease activities of Artemis and the Artemis: DNA-PKcs complex Artemis is a vertebrate nuclease with both endo- and exonuclease activities that acts on a wide rang...
  2. Editorial: CRISPR in Nucleic Acids Research
  3. RNAontheBENCH: computational and empirical resources for benchmarking RNAseq quantification and differential expression methods RNA sequencing (RNAseq) has become the method of choice for transcriptome analysis, yet no consensus...
  4. LDSS-P: an advanced algorithm to extract functional short motifs associated with coordinated gene expression Identifying functional elements in promoter sequences is a major goal in computational and experimen...
  5. SPATIAL: A System-level PAThway Impact AnaLysis approach The goal of pathway analysis is to identify the pathways that are significantly impacted when a biol...

Aviso de cookies: Usamos cookies propias y de terceros para mejorar nuestros servicios, para análisis estadístico y para mostrarle publicidad. Si continua navegando consideramos que acepta su uso en los términos establecidos en la Política de cookies.