1) La descarga del recurso depende de la página de origen
2) Para poder descargar el recurso, es necesario ser usuario registrado en Universia


Opción 1: Descargar recurso

Detalles del recurso

Descripción

Inulin is a non-digestible carbohydrate fructan polymer consisting mainly of β (1→2) fructosyl fructose links. Enzymatic hydrolysis of inulin by inulinase results in the production of low D.P (degree of polymerization) oligosaccharides also called fructooligosaccharides(FOS). Isolation of inulin from chicory root (Chicorium intybus) was achieved by first, extraction using deionized water (600C), followed by carbonation (0.1 M Ca(OH)2 and CO2 gas). This was filtered in order to remove the non sugars, thereafter, treated successfully with polyamide 6 powder. A cation exchanger and an anion exchanger were used to further exclude other components such as tannins and pigments. The extracted inulin was quantified using the Somogyi-Nelson colourimetric assay. Chicory root (207 g, 30 % being water) yielded 30 g of the raw extract. A 100 mg of the raw extract was assayed and found to contain 11 % yield of inulin which was 80.2 % in purity and 4 % free fructose. Analysis of the crude and purified inulin extracts on the MALDI TOF spectrometry showed the samples to have a DP of 2 to 22 and 2 to 27 respectively. Maximum inulinase production from Aspergillus niger grown on inulin was observed after 60 hours. The enzyme activity was found to be 1.168 U/ml with a temperature and pH optimum of 30 °C and 7.7 respectively. The enzyme proved to be unstable as it progressively lost its total activity during attempts at purification.

Pertenece a

Rhodes eResearch Repository  

Autor(es)

Mavumengwana, Vuyo Bhongolethu. - 

Id.: 55231075

Versión: 1.0

Estado: Final

Tipo:  text - 

Palabras claveQD415 Biochemistry - 

Tipo de recurso: Thesis  -  NonPeerReviewed  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

Formatos:  text - 

Requerimientos técnicos:  Browser: Any - 

Relación: [References] http://eprints.ru.ac.za/2745/

Fecha de contribución: 01-may-2012

Contacto:

Localización:
* Mavumengwana, Vuyo Bhongolethu. (2005) Isolation, purification and characterization of inulin and fructooligosaccharides from chicorium intybus and inulinase from aspergillus niger. Masters thesis, Rhodes University.

Otros recursos que te pueden interesar

  1. On modal extensions of Product fuzzy logic In this article, we study modal extensions of Product fuzzy logic with both algebraic semantics and ...
  2. On minimization of the number of branches in branch-and-bound algorithms for the maximum clique problem When searching for a maximum clique in a graph G, branch-and-bound algorithms in the literature usua...
  3. Non-linear model predictive control applied to PEM fuel cells: anode pressure and humidity regulation A thesis submitted in fulfilment of the requirements for the degree of Master in Automatic Control a...
  4. Managing different sources of uncertainty in a BDI framework in a principled way with tractable fragments The Belief-Desire-Intention (BDI) architecture is a practical approach for modelling large-scale int...
  5. Immunohistochemical localization of basic fibroblast growth factor in ependymal cells of the rat lateral and third ventricles The presence of basic fibroblast growth factor (bFGF) in the third and the lateral ventricular epend...

Otros recursos de la mismacolección

No existen otros recursos

Aviso de cookies: Usamos cookies propias y de terceros para mejorar nuestros servicios, para análisis estadístico y para mostrarle publicidad. Si continua navegando consideramos que acepta su uso en los términos establecidos en la Política de cookies.