1) La descarga del recurso depende de la página de origen
2) Para poder descargar el recurso, es necesario ser usuario registrado en Universia


Opción 1: Descargar recurso

Detalles del recurso

Descripción

Inulin is a non-digestible carbohydrate fructan polymer consisting mainly of β (1→2) fructosyl fructose links. Enzymatic hydrolysis of inulin by inulinase results in the production of low D.P (degree of polymerization) oligosaccharides also called fructooligosaccharides(FOS). Isolation of inulin from chicory root (Chicorium intybus) was achieved by first, extraction using deionized water (600C), followed by carbonation (0.1 M Ca(OH)2 and CO2 gas). This was filtered in order to remove the non sugars, thereafter, treated successfully with polyamide 6 powder. A cation exchanger and an anion exchanger were used to further exclude other components such as tannins and pigments. The extracted inulin was quantified using the Somogyi-Nelson colourimetric assay. Chicory root (207 g, 30 % being water) yielded 30 g of the raw extract. A 100 mg of the raw extract was assayed and found to contain 11 % yield of inulin which was 80.2 % in purity and 4 % free fructose. Analysis of the crude and purified inulin extracts on the MALDI TOF spectrometry showed the samples to have a DP of 2 to 22 and 2 to 27 respectively. Maximum inulinase production from Aspergillus niger grown on inulin was observed after 60 hours. The enzyme activity was found to be 1.168 U/ml with a temperature and pH optimum of 30 °C and 7.7 respectively. The enzyme proved to be unstable as it progressively lost its total activity during attempts at purification.

Pertenece a

Rhodes eResearch Repository  

Autor(es)

Mavumengwana, Vuyo Bhongolethu. - 

Id.: 55231075

Versión: 1.0

Estado: Final

Tipo:  text - 

Palabras claveQD415 Biochemistry - 

Tipo de recurso: Thesis  -  NonPeerReviewed  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

Formatos:  text - 

Requerimientos técnicos:  Browser: Any - 

Relación: [References] http://eprints.ru.ac.za/2745/

Fecha de contribución: 01-may-2012

Contacto:

Localización:
* Mavumengwana, Vuyo Bhongolethu. (2005) Isolation, purification and characterization of inulin and fructooligosaccharides from chicorium intybus and inulinase from aspergillus niger. Masters thesis, Rhodes University.

Otros recursos que te pueden interesar

  1. Immunohistochemical study of the endocrine cells in the stomach and pyloric caeca of the mountain trout, Salmo trutta macrostigma The endocrine cells of Salmo trutta macrostigma stomach and pyloric caeca have been investigated usi...
  2. In vitro effects of Sargassum latifolium (Agardeh, 1948) against selected bacterial pathogens of shrimp During the last decade, shrimp aquaculture has developed rapidly in many developing countries. Antib...
  3. Sperm quality, egg size, fecundity and their relationships with fertilization rate of grass carp (Ctenopharyngodon idella) Gametes were collected by abdominal massage from 15 anesthesized male and female grass carp. In coll...
  4. State of Mnemiopsis leidyi (Ctenophora: Lobata) and mesozooplankton in Iranian waters of the Caspian Sea during 2008 in comparison with previous surveys Mnemiopsis leidyi which was accidentally introduced into the Caspian Sea in 1999 and since then has ...
  5. Seasonal variations of fat and fatty acid composition in muscle tissues of Mediterranean octopuses The effects of seasons on lipid and fatty acid profiles of muscle types (mantle and arm) of Mediterr...

Otros recursos de la mismacolección

No existen otros recursos

Aviso de cookies: Usamos cookies propias y de terceros para mejorar nuestros servicios, para análisis estadístico y para mostrarle publicidad. Si continua navegando consideramos que acepta su uso en los términos establecidos en la Política de cookies.