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Venation patterning in leaves is a major determinant of photosynthesis efficiency because of its dependency on vascular transport of photoassimilates, water, and minerals. Arabidopsis thaliana grown in microgravity show delayed growth and leaf maturation. Gene expression data from the roots, hypocotyl, and leaves of A. thaliana grown during spaceflight vs. ground control analyzed by Affymetrix microarray are available through NASA's GeneLab (GLDS-7). We analyzed the data for differential expression of genes in leaves resulting from the effects of spaceflight on vascular patterning. Two genes were found by preliminary analysis to be upregulated during spaceflight that may be related to vascular formation. The genes are responsible for coding an ARGOS like protein (potentially affecting cell elongation in the leaves), and an F-box/kelch-repeat protein (possibly contributing to protoxylem specification). Further analysis that will focus on raw data quality assessment and a moderated t-test may further confirm upregulation of the two genes and/or identify other gene candidates. Plants defective in these genes will then be assessed for phenotype by the mapping and quantification of leaf vascular patterning by NASA's VESsel GENeration (VESGEN) software to model specific vascular differences of plants grown in spaceflight.

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NASA Technical Reports Server (NTRS)  

Autor(es)

Parsons -  Wingerter, P. -  Weitzeal, A. J. -  Wyatt, S. E. - 

Id.: 69703668

Versión: 1.0

Estado: Final

Tipo:  application/pdf - 

Palabras claveLife Sciences (General) - 

Cobertura:  Unclassified, Unlimited, Publicly available - 

Tipo de recurso: ARC-E-DAA-TN36565-2  -  Annual Meeting of the American Society for Gravitational and Space Research (ASGSR 2016); 26-29 Oct. 2016 ; Cleveland, OH; United States  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

: Copyright, Distribution as joint owner in the copyright

Formatos:  application/pdf - 

Requerimientos técnicos:  Browser: Any - 

Relación: [IsBasedOn] CASI

Fecha de contribución: 11-mar-2017

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