1) La descarga del recurso depende de la página de origen
2) Para poder descargar el recurso, es necesario ser usuario registrado en Universia

Opción 1: Descargar recurso

Detalles del recurso


Chemotaxis allows bacteria to approach sources of attractant chemicals or to avoid sources of repellent chemicals. Bacteria constantly monitor the concentration of specific chemoeffectors by comparing the current concentration to the concentration detected a few seconds earlier. This comparison determines the net direction of movement. Although multiple, competing gradients often coexist in nature, conventional approaches for investigating bacterial chemotaxis are suboptimal for quantifying migration in response to concentration gradients of attractants and repellents. Here, we describe the development of a microfluidic chemotaxis model for presenting precise and stable concentration gradients of chemoeffectors to bacteria and quantitatively investigating their response to the applied gradient. The device is versatile in that concentration gradients of any desired absolute concentration and gradient strength can be easily generated by diffusive mixing. The device is demonstrated using the response of Escherichia coli RP437 to gradients of amino acids and nickel ions.

Pertenece a

PubMed Central (PMC3 - NLM DTD)  


Englert, Derek L. -  Manson, Michael D. -  Jayaraman, Arul - 

Id.: 55209382

Idioma: inglés  - 

Versión: 1.0

Estado: Final

Palabras claveMicrobiology - 

Tipo de recurso: Text  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

Requerimientos técnicos:  Browser: Any - 

Fecha de contribución: 20-abr-2012



Otros recursos del mismo autor(es)

  1. Parallel screening of FDA-approved antineoplastic drugs for identifying sensitizers of TRAIL-induced apoptosis in cancer cells



    Tumor Necrosis Factor-α Related Apoptosis Inducing Ligand (TRAI...

  2. Indole is an inter-species biofilm signal mediated by SdiA



    As a stationary phase signal, indole is secreted in large quant...

  3. Integrated modeling and experimental approach for determining transcription factor profiles from fluorescent reporter data



    The development of quantitative models of signal transduction, ...

  4. Chemotaxis of Escherichia coli to Norepinephrine (NE) Requires Conversion of NE to 3,4-Dihydroxymandelic Acid Norepinephrine (NE), the primary neurotransmitter of the sympathetic nervous system, has been report...
  5. Microbiome-Derived Tryptophan Metabolites and Their Aryl Hydrocarbon Receptor-Dependent Agonist and Antagonist Activities The tryptophan metabolites indole, indole-3-acetate, and tryptamine were identified in mouse cecal e...

Otros recursos de la misma colección

  1. Studying DNA Looping by Single-Molecule FRET
  2. Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli
  3. Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis The extracellular matrix (ECM) is a complex meshwork of cross-linked proteins that provides biophysi...
  4. Establishment of a Clinically Relevant Ex Vivo Mock Cataract Surgery Model for Investigating Epithelial Wound Repair in a Native Microenvironment The major impediment to understanding how an epithelial tissue executes wound repair is the limited ...
  5. Stereotaxic Infusion of Oligomeric Amyloid-beta into the Mouse Hippocampus Alzheimer’s disease is a neurodegenerative disease affecting the aging population. A key neuropathol...

Aviso de cookies: Usamos cookies propias y de terceros para mejorar nuestros servicios, para análisis estadístico y para mostrarle publicidad. Si continua navegando consideramos que acepta su uso en los términos establecidos en la Política de cookies.