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Descripción

The rhesus macaque model is currently the best available model for HIV-AIDS with respect to understanding the pathogenesis as well as for the development of vaccines and therapeutics1,2,3. Here, we describe a method for the detailed phenotypic and functional analyses of cellular immune responses, specifically intracellular cytokine production by CD4+ and CD8+ T cells as well as the individual memory subsets. We obtained precise quantitative and qualitative measures for the production of interferon gamma (INF-) and interleukin (IL) -2 in both CD4+ and CD8+ T cells from the rhesus macaque PBMC stimulated with PMA plus ionomycin (PMA+I). The cytokine profiles were different in the different subsets of memory cells. Furthermore, this protocol provided us the sensitivity to demonstrate even minor fractions of antigen specific CD4+ and CD8+ T cell subsets within the PBMC samples from rhesus macaques immunized with an HIV envelope peptide cocktail vaccine developed in our laboratory. The multicolor flow cytometry technique is a powerful tool to precisely identify different populations of T cells 4,5 with cytokine-producing capability6 following non-specific or antigen-specific stimulation 5,7.

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PubMed Central (PMC3 - NLM DTD)  

Autor(es)

He, Hong -  Courtney, Amy N. -  Wieder, Eric -  Sastry, K. Jagannadha - 

Id.: 55213017

Idioma: inglés  - 

Versión: 1.0

Estado: Final

Palabras claveJoVE Immunology - 

Tipo de recurso: Text  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

Requerimientos técnicos:  Browser: Any - 

Fecha de contribución: 23-abr-2012

Contacto:

Localización:

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  3. Research Article Sparse Representation for Tumor Classification Based on Feature Extraction Using Latent Low-Rank Representation Copyright © 2014 Bin Gan et al.This is an open access article distributed under the Creative Commons...
  4. Food protein-induced proctocolitis: Is this allergic disorder a reality or a phantom in neonates? The etiology of small and fresh rectal bleeding in neonates who are not sick is usually unknown; the...
  5. Background This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-C...

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  4. Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome Trypanosoma brucei is a kinetoplastid parasite that causes human African trypanosomiasis (HAT), or s...
  5. Isolation of Murine Lymph Node Stromal Cells Secondary lymphoid organs including lymph nodes are composed of stromal cells that provide a structu...

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