Detalles del recurso
Pertenece a:
PubMed Central (PMC3 - NLM DTD)
Descripción: The rhesus macaque model is currently the best available model for HIV-AIDS with respect to understanding the pathogenesis as well as for the development of vaccines and therapeutics1,2,3. Here, we describe a method for the detailed phenotypic and functional analyses of cellular immune responses, specifically intracellular cytokine production by CD4+ and CD8+ T cells as well as the individual memory subsets. We obtained precise quantitative and qualitative measures for the production of interferon gamma (INF-) and interleukin (IL) -2 in both CD4+ and CD8+ T cells from the rhesus macaque PBMC stimulated with PMA plus ionomycin (PMA+I). The cytokine profiles were different in the different subsets of memory cells. Furthermore, this protocol provided us the sensitivity to demonstrate even minor fractions of antigen specific CD4+ and CD8+ T cell subsets within the PBMC samples from rhesus macaques immunized with an HIV envelope peptide cocktail vaccine developed in our laboratory. The multicolor flow cytometry technique is a powerful tool to precisely identify different populations of T cells 4,5 with cytokine-producing capability6 following non-specific or antigen-specific stimulation 5,7.
Autor(es): He, Hong - Courtney, Amy N. - Wieder, Eric - Sastry, K. Jagannadha -
Id.: 55213017
Idioma:
inglés
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Versión: 1.0
Estado: Final
Palabras clave: JoVE Immunology -
Tipo de recurso:
Text
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Tipo de Interactividad: Expositivo
Nivel de Interactividad: muy bajo
Audiencia:
Estudiante
- Profesor
- Autor
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Estructura: Atomic
Coste: no
Copyright: sí
Requerimientos técnicos: Browser: Any -
Fecha de contribución: 23-abr-2012
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