1) La descarga del recurso depende de la página de origen
2) Para poder descargar el recurso, es necesario ser usuario registrado en Universia


Opción 1: Descargar recurso

Opción 2: Descargar recurso

Detalles del recurso

Descripción

Human and animal studies have converged to suggest that caffeine consumption prevents memory deficits in aging and Alzheimer’s disease through the antagonism of adenosine A2A receptors (A2ARs). To test if A2AR activation in the hippocampus is actually sufficient to impair memory function and to begin elucidating the intracellular pathways operated by A2AR, we have developed a chimeric rhodopsin-A2AR protein (optoA2AR), which retains the extracellular and transmembrane domains of rhodopsin (conferring light responsiveness and eliminating adenosine-binding pockets) fused to the intracellular loop of A2AR to confer specific A2AR signaling. The specificity of the optoA2AR signaling was confirmed by light-induced selective enhancement of cAMP and phospho-mitogen-activated protein kinase (p-MAPK) (but not cGMP) levels in human embryonic kidney 293 (HEK293) cells, which was abolished by a point mutation at the C terminal of A2AR. Supporting its physiological relevance, optoA2AR activation and the A2AR agonist CGS21680 produced similar activation of cAMP and p-MAPK signaling in HEK293 cells, of p-MAPK in the nucleus accumbens and of c-Fos/phosphorylated-CREB (p-CREB) in the hippocampus, and similarly enhanced long-term potentiation in the hippocampus. Remarkably, optoA2AR activation triggered a preferential p-CREB signaling in the hippocampus and impaired spatial memory performance, while optoA2AR activation in the nucleus accumbens triggered MAPK signaling and modulated locomotor activity. This shows that the recruitment of intracellular A2AR signaling in the hippocampus is sufficient to trigger memory dysfunction. Furthermore, the demonstration that the biased A2AR signaling and functions depend on intracellular A2AR loops prompts the possibility of targeting the intracellular A2AR-interacting partners to selectively control different neuropsychiatric behaviors.

Pertenece a

DSpace at MIT  

Autor(es)

Li, P. -  Rial, D. -  Canas, P. M. -  Yoo, J. -  H. -  Li, W. -  Zhou, X. -  Wang, Y. -  van Westen, G. J. P. -  Payen, M. -  P. -  Augusto, E. -  Gonçalves, N. -  Tomé, A. R. -  Li, Z. -  Wu, Z. -  Hou, X. -  Zhou, Y. -  PIJzerman, Ad -  Boyden, Edward Stuart -  Cunha, R. A. -  Qu, J. -  Chen, J. -  F. - 

Id.: 65994567

Idioma: inglés (Estados Unidos)  - 

Versión: 1.0

Estado: Final

Tipo de recurso: Article  -  http://purl.org/eprint/type/JournalArticle  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

: Creative Commons Attribution-Noncommercial-Share Alike

Requerimientos técnicos:  Browser: Any - 

Relación: [IsBasedOn] PMC
[References] http://dx.doi.org/10.1038/mp.2014.182
[References] Molecular Psychiatry

Fecha de contribución: 10-dic-2016

Contacto:

Localización:
* 1359-4184
* 1476-5578
* Li, P., D. Rial, P.M. Canas, J.-H. Yoo, W. Li, X. Zhou, Y. Wang, G.J.P. van Westen, M.-P. Payen, E. Augusto, et al. "Optogenetic activation of intracellular adenosine A2A receptor signaling in the hippocampus is sufficient to trigger CREB phosphorylation and impair memory." Molecular Psychiatry (2015) 20, pp.1339-1349.
* OPEN_ACCESS_POLICY

Otros recursos del mismo autor(es)

  1. De Copas con Ciencia- Aragón Radio- Ágora Entrevista a Germán F. de la Fuente en el Programa Ágora de Radio Aragón.
  2. Ciclo del azufre en la plataforma continental mediterránea Seminario científico del 15 al 18 de Marzo de 1983, Cádiz.-- 14 pages, 6 figures
  3. A study of quasar selection in the supernova fields of the Dark Energy Survey We present a study of quasar selection using the supernova fields of the Dark Energy Survey (DES). W...
  4. Farthest neighbor : the distant Milky Way satellite Eridanus II We present Magellan/IMACS spectroscopy of the recently discovered Milky Way satellite EridanusII (Er...
  5. Sloan Digital Sky Survey IV : mapping the Milky Way, nearby galaxies, and the distant universe We describe the Sloan Digital Sky Survey IV (SDSS-IV), a project encompassing three major spectrosco...

Otros recursos de la mismacolección

  1. UV-solvent annealing for morphology and orientation control in self-assembled PS-PDMS thin films The response of polystyrene-block-poly(dimethylsiloxane) (PS-b-PDMS) thin films to UV exposure durin...
  2. Interface electronic structure at the topological insulator–ferrimagnetic insulator junction An interface electron state at the junction between a three-dimensional topological insulator film, ...
  3. Temporal Evolution of Auto-Oscillations in an Yttrium-Iron-Garnet/Platinum Microdisk Driven by Pulsed Spin Hall Effect-Induced Spin-Transfer Torque The temporal evolution of pulsed spin Hall effect-spin transfer torque (SHE-STT) driven auto-oscilla...
  4. Control and local measurement of the spin chemical potential in a magnetic insulator The spin chemical potential characterizes the tendency of spins to diffuse. Probing this quantity co...
  5. The role of Snell’s law for a magnonic majority gate In the fifty years since the postulation of Moore's Law, the increasing energy consumption in silico...

Aviso de cookies: Usamos cookies propias y de terceros para mejorar nuestros servicios, para análisis estadístico y para mostrarle publicidad. Si continua navegando consideramos que acepta su uso en los términos establecidos en la Política de cookies.