1) La descarga del recurso depende de la página de origen
2) Para poder descargar el recurso, es necesario ser usuario registrado en Universia


Opción 1: Descargar recurso

Opción 2: Descargar recurso

Detalles del recurso

Descripción

Nucleoside diphosphate kinase (NDPK) is a ubiquitous enzyme that catalyzes the transfer of the γ-phosphoryl group from a nucleoside triphosphate to a nucleoside diphosphate. In this study, we examined the subcellular localization, tissue-specific gene expression, and enzymatic characteristics of three rice NDPK isozymes (OsNDPK1-OsNDPK3). Sequence comparison of the three OsNDPKs suggested differential subcellular localization. Transient expression of green fluorescence protein-fused proteins in onion cells indicated that OsNDPK2 and OsNDPK3 are localized to plastid and mitochondria respectively, while OsNDPK1 is localized to the cytosol. Expression analysis indicated that all the OsNDPKs are expressed in the leaf, leaf sheath, and immature seeds, except for OsNDPK1, in the leaf sheath. Recombinant OsNDPK2 and OsNDPK3 showed lower optimum pH and higher stability under acidic pH than OsNDPK1. In ATP formation, all the OsNDPKs displayed lower K(m) values for the second substrate, ADP, than for the first substrate, NTP, and showed lowest and highest K(m) values for GTP and CTP respectively.

Pertenece a

Hokkaido University Collection of Scholarly and Academic Papers  

Autor(es)

Kihara, Akihiko -  Saburi, Wataru -  Wakuta, Shinji -  Kim, Myung -  Hee -  Hamada, Shigeki -  Ito, Hiroyuki -  Imai, Ryozo -  Matsui, Hirokazu - 

Id.: 70417787

Idioma: inglés  - 

Versión: 1.0

Estado: Final

Palabras clavenucleoside diphosphate kinase - 

Tipo de recurso: article (author version)  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

: This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry on 2011 Sep, available online: http://wwww.tandfonline.com/10.1271/bbb.110285.

Requerimientos técnicos:  Browser: Any - 

Fecha de contribución: 11-nov-2017

Contacto:

Localización:
* Bioscience, Biotechnology, and Biochemistry, 75(9): 1740-1745

Otros recursos del mismo autor(es)

  1. Gender and Socioeconomic Status in Relation to Weight Perception and Weight Control Behavior in Korean Adults Aim In Korea, obesity is more prevalent among men and lower socioeconomic groups. To explain this ob...
  2. Generation of Ca2+-independent sortase A mutants with enhanced activity for protein and cell surface labeling Sortase A, a calcium-dependent transpeptidase derived from Staphylococcus aureus, is used in a broad...
  3. Spatial Charge Configuration Regulates Nanoparticle Transport and Binding Behavior In Vivo Detailed arrangements: A new set of zwitterionic quantum dots were synthesized and used to study the...
  4. Sports and ‘Minorities’: Negotiating the Olympic Model International audience
  5. Effets du diagnostic d’infection au VIH sur la participation sociale : enjeux de l’application du Processus de Production du Handicap à une maladie chronique International audience

Otros recursos de la mismacolección

  1. Macrocarpal C isolated from Eucalyptus globulus inhibits dipeptidyl peptidase 4 in an aggregated form Dipeptidyl peptidase 4 (DPP-4) inhibitors are used for the treatment of type-2 diabetes mellitus. Va...
  2. Roles of genes regulated by two paternally methylated imprinted regions on chromosomes 7 and 12 in mouse ontogeny We studied the longevity of mice produced without sperm using the genomes of oocytes that are alread...
  3. Difference in sensitivity to culture condition between In Vitro fertilized and somatic cell nuclear transfer embryos in pigs We evaluated the developmental competence of somatic cell nuclear transfer (SCNT) embryos using in v...
  4. Acetylation level of histone H3 in early embryonic stages affects subsequent development of miniature pig somatic cell nuclear transfer embryos Successful cloning by somatic cell nuclear transfer (SCNT) requires a reprogramming process in which...
  5. Involvement of insulin-like growth factor 2 in angiogenic factor transcription in bi-maternal mouse conceptuses Imprinted genes in which only one of the two parental chromosome copies is expressed have a substant...

Aviso de cookies: Usamos cookies propias y de terceros para mejorar nuestros servicios, para análisis estadístico y para mostrarle publicidad. Si continua navegando consideramos que acepta su uso en los términos establecidos en la Política de cookies.