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A procedure for the preparation, purification and analysis of small, BSA-coated carboxylate-modified latex (CML) particles, suitable for use in in vivo studies has been developed. Following conjugation, uptake of unbound BSA by Amberlite XAD-8 non-ionic adsorbent beads has been shown to be an effective method by which unbound protein ligands may be removed from coated latex preparations.

Pertenece a

White Rose Research Online  


Goode, NP -  Davison, AM -  Gowland, G -  Shires, M - 

Id.: 55216250

Versión: 1.0

Estado: Final

Tipo de recurso: Article  -  NonPeerReviewed  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

Requerimientos técnicos:  Browser: Any - 

Relación: [References] http://dx.doi.org/10.1016/0022-1759(86)90500-4
[References] http://eprints.whiterose.ac.uk/43873/

Fecha de contribución: 20-feb-2016


* Goode, NP, Davison, AM, Gowland, G and Shires, M (1986) Preparation, purification and analysis of BSA-coated latex particles for invivo studies. Non-ionic adsorbance of unbound protein ligand. Journal of Immunological Methods, 92 (1). 31 - 35 . ISSN 0022-1759

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