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[EN] Monoclonal antibodies (MAbs) for the carbamate insecticide carbaryl (1-naphthyl N-methylcarbamate) were obtained by immunizing mice with 6-[[(1-naphthyloxy)carbonyl]amino]hexanoic acid (3, CNH) conjugated to bovine serum albumin (BSA). The hapten synthesis strategy was designed to preserve the carbamate group characteristic of N-methylcarbamate pesticides. The most promising MAb (LIB/CNH-3.6) was characterized by using an indirect homologous antigen-coated ELISA. With this assay format, LIB/CNH-3.6 showed a high affinity for carbaryl (I-50 = 3 6 nM) and minimum cross-reactivities for several N-methylcarbamates and 1-naphthol, the main carbaryl metabolite. Both affinity and specificity achieved by LIB/CNH-3.6 meant a great improvement with respect to the features displayed by the serum of the mouse from which the MAb was derived. This MAb appears to be a very promising immunoreagent for the future development of a specific and sensitive quantitative ELISA for carbaryl, and the applied hapten synthesis strategy is proposed as a general procedure to obtain immunogenic conjugates for other N-methylcarbamate pesticides.

Pertenece a

RiuNet. Repositorio institucional de la Universitat de Valencia  

Autor(es)

Abad Fuentes, Antonio -  Montoya, Ángel - 

Id.: 71077833

Idioma: eng  - 

Versión: 1.0

Estado: Final

Palabras claveCarbaryl - 

Tipo de recurso: info:eu-repo/semantics/article  -  info:eu repo/semantics/publishedVersion  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

: Reserva de todos los derechos

Requerimientos técnicos:  Browser: Any - 

Relación: [References] Journal of Agricultural and Food Chemistry
[References] https://doi.org/10.1021/jf00044a047

Fecha de contribución: 14-mar-2018

Contacto:

Localización:
* 0021-8561
* 10.1021/jf00044a047

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