1) La descarga del recurso depende de la página de origen
2) Para poder descargar el recurso, es necesario ser usuario registrado en Universia

Opción 1: Descargar recurso

Detalles del recurso


[EN] Monoclonal antibodies (MAbs) for the carbamate insecticide carbaryl (1-naphthyl N-methylcarbamate) were obtained by immunizing mice with 6-[[(1-naphthyloxy)carbonyl]amino]hexanoic acid (3, CNH) conjugated to bovine serum albumin (BSA). The hapten synthesis strategy was designed to preserve the carbamate group characteristic of N-methylcarbamate pesticides. The most promising MAb (LIB/CNH-3.6) was characterized by using an indirect homologous antigen-coated ELISA. With this assay format, LIB/CNH-3.6 showed a high affinity for carbaryl (I-50 = 3 6 nM) and minimum cross-reactivities for several N-methylcarbamates and 1-naphthol, the main carbaryl metabolite. Both affinity and specificity achieved by LIB/CNH-3.6 meant a great improvement with respect to the features displayed by the serum of the mouse from which the MAb was derived. This MAb appears to be a very promising immunoreagent for the future development of a specific and sensitive quantitative ELISA for carbaryl, and the applied hapten synthesis strategy is proposed as a general procedure to obtain immunogenic conjugates for other N-methylcarbamate pesticides.

Pertenece a

RiuNet. Repositorio institucional de la Universitat de Valencia  


Abad Fuentes, Antonio -  Montoya, Ángel - 

Id.: 71077833

Idioma: eng  - 

Versión: 1.0

Estado: Final

Palabras claveCarbaryl - 

Tipo de recurso: info:eu-repo/semantics/article  -  info:eu repo/semantics/publishedVersion  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

: Reserva de todos los derechos

Requerimientos técnicos:  Browser: Any - 

Relación: [References] Journal of Agricultural and Food Chemistry
[References] https://doi.org/10.1021/jf00044a047

Fecha de contribución: 14-mar-2018


* 0021-8561
* 10.1021/jf00044a047

Otros recursos del mismo autor(es)

  1. High Fundamental Frequency Quartz Crystal Microbalance (HFF-QCM) immunosensor for pesticide detection in honey [EN] Quantification of chemical residues in honey is a market requirement to ensure consumer safety....
  2. A Comprehensive Overview on The Application of Flow-Injection in Immunoanalysis [EN] Flow injection analysis (FIA) is a technique that allows the automatic analysis of a large num...
  3. Application of a monoclonal-based immunoassay for the screening of imazalil in fruit juices International audience
  4. Comparison of a monoclonal antibody-based enzyme-linked immunosorbent assay and gas chromatography for the determination of nicotine in cigarette smoke condensates [EN] A competitive enzyme-linked immunosorbent assay (ELISA) based on monoclonal antibodies was deve...
  5. Development of a Chlorpyrifos Immunoassay Using Antibodies Obtained from a Simple Hapten Design Manclus Ciscar, JJ.; Primo Millo, J.; Montoya, Á. (1994). Development of a Chlorpyrifos Immunoassay ...

Otros recursos de la mismacolección

  1. Efficient simulation of the pantograph-catenary dynamic interaction. Catenary optimisation and installation error analysis El modelado y la simulación de la interacción dinámica entre el pantógrafo y la catenaria se ha conv...
  2. The role of monitoring sustainable drainage systems for promoting transition towards regenerative urban built environments: a case study in the Valencian region, Spain [EN] Sustainable drainage systems are an alternative and holistic approach to conventional urban sto...
  3. Influence of VOF technique, turbulence model and discretization scheme on the numerical simulation of the non-aerated, skimming flow in stepped spillways [EN] An accurate description of the hydrodynamics in the non-aerated region of the skimming flow on ...
  4. Combined use of relative drought indices to analyze climate change impact on meteorological and hydrological droughts in a Mediterranean basin [EN] Standardized drought indices have been traditionally used to identify and assess droughts becau...
  5. Changes in the process performance, sludge production and microbial activity in an activated sludge reactor with addition of a metabolic uncoupler under different operating conditions [EN] Sludge production in wastewater treatment plants is nowadays a big concern due to the high prod...

Aviso de cookies: Usamos cookies propias y de terceros para mejorar nuestros servicios, para análisis estadístico y para mostrarle publicidad. Si continua navegando consideramos que acepta su uso en los términos establecidos en la Política de cookies.