1) La descarga del recurso depende de la página de origen
2) Para poder descargar el recurso, es necesario ser usuario registrado en Universia


Opción 1: Descargar recurso

Detalles del recurso

Descripción

Important questions in biology have emerged recently concerning the timing of transcription in living cells. Studies on clonal cell lines have shown that transcription is often pulsatile and stochastic, with implications for cellular differentiation. Currently, information regarding transcriptional activity at cellular resolution within a physiological context remains limited. To investigate single-cell transcriptional activity in real-time in living tissue we used bioluminescence imaging of pituitary tissue from transgenic rats in which luciferase gene expression is driven by a pituitary hormone gene promoter. We studied fetal and neonatal pituitary tissue to assess whether dynamic patterns of transcription change during tissue development. We show that gene expression in single cells is highly pulsatile at the time endocrine cells first appear but becomes stabilised as the tissue develops in early neonatal life. This stabilised transcription pattern might depend upon tissue architecture or paracrine signalling, as isolated cells, generated from enzymatic dispersion of the tissue, display pulsatile luminescence. Nascent cells in embryonic tissue also showed coordinated transcription activity over short distances further indicating that cellular context is important for transcription activity. Overall, our data show that cells alter their patterns of gene expression according to their context and developmental stage, with important implications for cellular differentiation.

Pertenece a

PubMed Central (PMC3 - NLM DTD)  

Autor(es)

Featherstone, Karen -  Harper, Claire V. -  McNamara, Anne -  Semprini, Sabrina -  Spiller, David G. -  McNeilly, Judith -  McNeilly, Alan S. -  Mullins, John J. -  White, Michael R. H. -  Davis, Julian R. E. - 

Id.: 55202184

Idioma: inglés  - 

Versión: 1.0

Estado: Final

Palabras claveResearch Articles - 

Tipo de recurso: Text  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

Requerimientos técnicos:  Browser: Any - 

Fecha de contribución: 16-abr-2012

Contacto:

Localización:

Otros recursos de la misma colección

  1. Akt signaling dynamics in individual cells The protein kinase Akt (for which there are three isoforms) is a key intracellular mediator of many ...
  2. Activated microglia cause reversible apoptosis of pheochromocytoma cells, inducing their cell death by phagocytosis Some apoptotic processes, such as phosphatidylserine exposure, are potentially reversible and do not...
  3. Assembly and maintenance of the flagellum attachment zone filament in Trypanosoma brucei Adhesion of motile flagella to the cell body in Trypanosoma brucei requires a filamentous cytoskelet...
  4. CALHM1 ion channel elicits amyloid-β clearance by insulin-degrading enzyme in cell lines and in vivo in the mouse brain Alzheimer's disease is characterized by amyloid-β (Aβ) peptide accumulation in the brain. CALHM1, a ...
  5. Differing roles of pyruvate dehydrogenase kinases during mouse oocyte maturation Pyruvate dehydrogenase kinases (PDKs) modulate energy homeostasis in multiple tissues and cell types...

Aviso de cookies: Usamos cookies propias y de terceros para mejorar nuestros servicios, para análisis estadístico y para mostrarle publicidad. Si continua navegando consideramos que acepta su uso en los términos establecidos en la Política de cookies.