Friday, April 18, 2014

 

 



Soy un nuevo usuario

Olvidé mi contraseña

Entrada usuarios

Lógica Matemáticas Astronomía y Astrofísica Física Química Ciencias de la Vida
Ciencias de la Tierra y Espacio Ciencias Agrarias Ciencias Médicas Ciencias Tecnológicas Antropología Demografía
Ciencias Económicas Geografía Historia Ciencias Jurídicas y Derecho Lingüística Pedagogía
Ciencia Política Psicología Artes y Letras Sociología Ética Filosofía


Pulsatile patterns of pituitary hormone gene expression change during development

1) La descarga del recurso depende de la página de origen
2) Para poder descargar el recurso, es necesario ser usuario
    registrado en Universia

  Descargar recurso

Detalles del recurso

Pertenece a: PubMed Central (PMC3 - NLM DTD)  

Descripción: Important questions in biology have emerged recently concerning the timing of transcription in living cells. Studies on clonal cell lines have shown that transcription is often pulsatile and stochastic, with implications for cellular differentiation. Currently, information regarding transcriptional activity at cellular resolution within a physiological context remains limited. To investigate single-cell transcriptional activity in real-time in living tissue we used bioluminescence imaging of pituitary tissue from transgenic rats in which luciferase gene expression is driven by a pituitary hormone gene promoter. We studied fetal and neonatal pituitary tissue to assess whether dynamic patterns of transcription change during tissue development. We show that gene expression in single cells is highly pulsatile at the time endocrine cells first appear but becomes stabilised as the tissue develops in early neonatal life. This stabilised transcription pattern might depend upon tissue architecture or paracrine signalling, as isolated cells, generated from enzymatic dispersion of the tissue, display pulsatile luminescence. Nascent cells in embryonic tissue also showed coordinated transcription activity over short distances further indicating that cellular context is important for transcription activity. Overall, our data show that cells alter their patterns of gene expression according to their context and developmental stage, with important implications for cellular differentiation.

Autor(es): Featherstone, Karen -  Harper, Claire V. -  McNamara, Anne -  Semprini, Sabrina -  Spiller, David G. -  McNeilly, Judith -  McNeilly, Alan S. -  Mullins, John J. -  White, Michael R. H. -  Davis, Julian R. E. - 

Id.: 55202184

Idioma: inglés  - 

Versión: 1.0

Estado: Final

Palabras claveResearch Articles - 

Tipo de recurso: Text  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

Requerimientos técnicos:  Browser: Any - 

Fecha de contribución: 16-abr-2012

Contacto:

Localización:


Otros recursos del mismo autor(es)

  1. Quantifying intrinsic and extrinsic noise in gene transcription using the linear noise approximation: An application to single cell data A central challenge in computational modeling of dynamic biological systems is parameter inference f...
  2. Perrault Syndrome Is Caused by Recessive Mutations in CLPP, Encoding a Mitochondrial ATP-Dependent Chambered Protease Perrault syndrome is a genetically and clinically heterogeneous autosomal-recessive condition charac...
  3. A urine-concentrating defect in 11β-hydroxysteroid dehydrogenase type 2 null mice In aldosterone target tissues, 11β-hydroxysteroid dehydrogenase type 2 (11βHSD2) is coexpressed with...
  4. The organisation of elastin and fibrillins 1 and 2 in the cruciate ligament complex Although elastin fibres and oxytalan fibres (bundles of microfibrils) have important mechanical, bio...
  5. Use of Fiber Optic Technology to Measure the Effects of Anesthesia on Luciferase Reaction Kinetics In vivo bioluminescent imaging (BLI) is a sensitive and reliable technique for studying gene express...

Otros recursos de la misma colección

  1. Invadolysin, a conserved lipid-droplet-associated metalloproteinase, is required for mitochondrial function in Drosophila Mitochondria are the main producers of ATP, the principal energy source of the cell, and reactive ox...
  2. Dynein light chain interaction with the peroxisomal import docking complex modulates peroxisome biogenesis in yeast Dynein is a large macromolecular motor complex that moves cargo along microtubules. A motor-independ...
  3. Ca2+ and calpain control membrane expansion during the rapid cell spreading of neutrophils Following adherence of neutrophils to the endothelium, neutrophils undergo a major morphological cha...
  4. Doa4 function in ILV budding is restricted through its interaction with the Vps20 subunit of ESCRT-III Assembly of the endosomal sorting complex required for transport (ESCRT)-III executes the formation ...
  5. Tumor suppressor p16INK4a inhibits cancer cell growth by downregulating eEF1A2 through a direct interaction The tumor suppressor protein p16INK4a is a member of the INK4 family of cyclin-dependent kinase (Cdk...

Valoración de los usuarios

No hay ninguna valoración para este recurso.Sea el primero en valorar este recurso.
 

Busque un recurso