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Background: Cellulose Binding Domains (CBD) were conjugated with fluorescein isothiocyanate (FITC). The surface concentration of the Binding Domains adsorbed on cellulose fibres was determined by fluorescence image analysis. Results: For a CBD-FITC concentration of 60 mg/L, a coating fraction of 78% and 110% was estimated for Portucel and Whatman fibres, respectively. For a saturating CBD concentration, using Whatman CF11 fibres, a surface concentration of 25.2 x 10(-13) mol/mm(2) was estimated, the equivalent to 4 protein monolayers. This result does not imply the existence of several adsorbed protein layers. Conclusion: It was verified that CBDs were able to penetrate the fibres, according to confocal microscopy and TEM-immunolabelling analysis. The surface concentration of adsorbed CBDs was greater on amorphous fibres (phosphoric acid swollen) than on more crystalline ones (Whatman CF11 and Sigmacell 20).

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Biblioteca Digital do IPB  

Autor(es)

Pinto, Ricardo -  Amaral, António L. -  Ferreira, Eugenio C. -  Mota, Manuel -  Vilanova, Manuel -  Ruel, Katia -  Gama, Miguel - 

Id.: 71382001

Idioma: eng  - 

Versión: 1.0

Estado: Final

Tipo de recurso: article  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

: openAccess

Requerimientos técnicos:  Browser: Any - 

Relación: [References] info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F6934%2F2001/PT

Fecha de contribución: 10-jun-2018

Contacto:

Localización:
* Pinto, Ricardo; Amaral, Antonio L.; Ferreira, Eugenio C.; Mota, Manuel; Vilanova, Manuel; Ruel, Katia; Gama, Miguel (2008) - Quantification of the CBD-FITC conjugates surface coating on cellulose fibres. BMC Biotechnology. ISSN 1472-6750. 8, p. 1-7
* 1472-6750
* 10.1186/1472-6750-8-1

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