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Most of studies in protein nanomechanics have used the atomic force microscope (AFM) in its force-measuring mode on immobilized protein repeats (polyproteins) as single-molecule markers. Here, we add imaging capabilities to a standard, state-of-the-art AFM >puller> and integrate the most powerful programs of analysis available for both AFM modes. This unique instrument allows high-resolution, quasi-simultaneous imaging/force spectroscopy in aqueous solution. We demonstrate its capabilities using polyproteins of a model system (titin I27 domain). This tool should greatly facilitate the development of a much needed universal functionalization system for AFM, one that should allow better sample control and an improved efficiency of protein immobilization. © 2007 American Institute of Physics.

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Autor(es)

Valbuena, A. -  Oroz, J. -  Vera, A.M. -  Gimeno, A. -  Gómez -  Herrero, J. -  Carrión -  Vázquez, Mariano Sixto - 

Id.: 70077645

Idioma: eng  - 

Versión: 1.0

Estado: Final

Tipo de recurso: Artículo  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

: closedAccess

Requerimientos técnicos:  Browser: Any - 

Relación: [References] Sí

Fecha de contribución: 07-sep-2017

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Localización:
* doi: 10.1063/1.2794732
* issn: 0034-6748
* Review of Scientific Instruments 78 (2007)

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