1) La descarga del recurso depende de la página de origen
2) Para poder descargar el recurso, es necesario ser usuario registrado en Universia

Detalles del recurso


Nicotinic acetylcholine receptors (nAChRs) are pentameric cation channels that are expressed in the mammalian central nervous system, the peripheral nervous system, and the neuromuscular junction. Eleven neuronal nAChR subunits have been identified in mammals (α2-7, α9-10, β2-4). Chronic administration of nicotine has been shown to increase total α4 and β2 subunit expression in specific brain regions. Menthol, a common cigarette flavoring, has also been shown to upregulate α4 and β2 subunit expression in the cortex, hippocampus, and striatum independent of nicotine co-administration. These data highlight the potential use of changes in β2 nAChR subunit expression as a marker for neuronal nicotine exposure when administered alone and when administered with additional bioactive compounds such as menthol. We investigated the effect of nicotine and of menthol in several murine brain regions on changes in total β2 subunit protein expression using western blot analysis. Absence of β2 immunoreactivity was confirmed in both β2 knockout and α4 knockout mice. To assess the effects of nicotine, male C57bl/6 mice were separated into two treatment groups: control (saline) and nicotine (2mg/kg/hr). Vehicle (ethanol) and menthol alone (2mg/kg/hr) were compared separately. All treatments were administered for 10–12 days. Nicotine-induced β2 immunoreactivity was significantly upregulated in isolated brain regions, including the cortex, hippocampus, midbrain, striatum, and thalamus. No change in immunoreactivity was observed in the habenula of mice treated chronically with nicotine. In response to chronic menthol treatment, a significant increase was only observed in the hypothalamus. These data indicate that nicotine and menthol differentially effect expression of nAChR subtypes in various regions of the brain. A comprehensive analysis of changes in β2 nAChR subunit expression in various brain regions will facilitate our understanding of the effects of nicotine exposure in the mammalian CNS. Additionally, the validation of a reliable marker for nicotine and menthol exposure in regions associated with various aspects of addiction and dependence would be invaluable in the pursuit of better cessation therapeutics. Using β2 expression as a marker of nicotine exposure will enable a better understanding of possible effects of nicotine and menthol.

Pertenece a

Caltech Authors  


Mulcahy, Matthew J. -  Wang, Jonathan H. -  Lester, Henry A. - 

Id.: 70099064

Versión: 1.0

Estado: Final

Tipo de recurso: Article  -  PeerReviewed  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

Requerimientos técnicos:  Browser: Any - 

Relación: [References] http://resolver.caltech.edu/CaltechAUTHORS:20170921-153023998
[References] http://authors.library.caltech.edu/81709/

Fecha de contribución: 22-sep-2017


* Mulcahy, Matthew J. and Wang, Jonathan H. and Lester, Henry A. (2017) Region specific proteomic analysis of murine brain after chronic nicotine or menthol. FASEB Journal, 31 (S1). Art. No. 991.1. ISSN 0892-6638. http://resolver.caltech.edu/CaltechAUTHORS:20170921-153023998

Otros recursos del mismo autor(es)

  1. Acute Ethanol Administration Upregulates Synaptic α4-Subunit of Neuronal Nicotinic Acetylcholine Receptors within the Nucleus Accumbens and Amygdala Alcohol and nicotine are two of the most frequently abused drugs, with their comorbidity well descri...
  2. TC299423, a Novel Agonist for Nicotinic Acetylcholine Receptors (E)-5-(Pyrimidin-5-yl)-1,2,3,4,7,8-hexahydroazocine (TC299423) is a novel agonist for nicotinic acet...
  3. Nicotine in the Endoplasmic Reticulum Nicotine activates plasma membrane (PM) nicotinic receptors (nAChRs), but also permeates into the en...
  4. The nicotinic 6 subunit gene determines variability in chronic pain sensitivity via cross-inhibition of P2X2/3 receptors International audience
  5. Incubator embedded cell culture imaging system (EmSight) based on Fourier ptychographic microscopy Multi-day tracking of cells in culture systems can provide valuable information in bioscience experi...

Otros recursos de la mismacolección

  1. Designing a market for tradable emissions permits The purpose of this chapter is to investigate the practicality of a system of tractable emissions pe...
  2. Some core assumptions in development economics The purpose of this paper is three-fold. It seeks to outline "conventional" development economics. I...
  3. Toroidal plasmoid generation via extreme hydrodynamic shear Saint Elmo’s fire and lightning are two known forms of naturally occurring atmospheric pressure plas...
  4. Market power and transferable property rights The appeal of using markets as a means of allocating scarce resources stems in large part from the a...
  5. Nash equilibrium search for the best alternative In a recent paper, Weitzman (Econometrica 47 (1979) 641–654) described a policy of “optimal search f...

Aviso de cookies: Usamos cookies propias y de terceros para mejorar nuestros servicios, para análisis estadístico y para mostrarle publicidad. Si continua navegando consideramos que acepta su uso en los términos establecidos en la Política de cookies.