1) La descarga del recurso depende de la página de origen
2) Para poder descargar el recurso, es necesario ser usuario registrado en Universia


Opción 1: Descargar recurso

Detalles del recurso

Descripción

Pleurotus ostreatus is not only the second edible mushroom worldwide but also a model fungus for delignification application, with the advantage of' growing on woody and nonwood y feedstocks. Its sequenced genome is available, and this gave us the opportunity to perform proteomic studies to identify the enzymes overproduced on lignocellulosic growth substrates. In the present study, the monokaryotic P.ostreatus was grown on poplar chips or wheat straw and the extracellular proteins were analyzed together with those from glucose medium. Using nano-Iiquid chromatography coupled to tandem rnass spectrometry or whole-protein hydrolyzate, over five-hundred proteins were identified. Thirty-four percent were unique or the straw cultures, while only 15 and 6% were unique of' the glucose and poplar cultures. respectively (20% were produced under the three conditions, and 19% were shared by the two lignocellulosic cultures). Serni-quantitalive analysis revealed that oxidoreductase represent the main protein type both in the poplar (39% of individual proteins) and straw (32%) secretomes. while carbohydrate-acting enzymes (CAZys) were only slightly over produced (14-1 %). La case 10 (LACC10) was the main protein in the two lignocellulose secretomes (10- 14%) and together with LACC2, LACC9, LACC6, versatile peroxidase 1 (VP 1) and manganese peroxidase 3, were strongly over produced in the lignocellulosic cultures seven CAZys were also among the top- 50 protein , but only CE 16 acetyl/cinnamoyl-esterase was overproduced on lignocellulosic When the woody and nonwoody lignocellulosic secretomes were compared, GH1 and GH3 Beta-glycosidases were more abundant on poplar and straw respectiyely and, among less abundant proteins, VP2 was overproduced on wheat straw. while VP3 was only found on poplar, The treated lignocellulosic substrates were analyzed by two- dimensional nuclear rnagnetic resonance (2D NMR), and a relative decrease of lignin to carbohydrate signals was observed in both samples, together with the disappearance of' some minor Iignin substructures and increase of sugar reducing-ends. We can conclude that oxidoreductases are strongly induced when P.ostreatus grows on both woody and nonwoody lignocellulosi subslrates One laccase occupied the first position in both secretomes. and three more were overproduced together with one VP and one MnP, suggesling an important role in lignocellulose degradation. Preferential removal of lignin vs carbohydrates was shown by 2D NMR, en agreement with the above secretomic results.

Pertenece a

Digital.CSIC  

Autor(es)

Fernández -  Fueyo, Elena -  Ruiz -  Dueñas, F. J. -  López -  Lucendo, María F. -  Pérez -  Boada, Marta -  Rencoret, Jorge -  Gutiérrez Suárez, Ana -  Pisabarro, Antonio G. -  Ramírez, Lucía -  Martínez, Ángel T. - 

Id.: 70472191

Idioma: eng  - 

Versión: 1.0

Estado: Final

Tipo de recurso: Póster  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

: closedAccess

Requerimientos técnicos:  Browser: Any - 

Relación: [References] Sí

Fecha de contribución: 23-nov-2017

Contacto:

Localización:
* OxiZymes 2016 (8th European Meeting on OxiZymes) 3–6 July 2016 (Wageningen) Netherlands

Otros recursos del mismo autor(es)

  1. Multivariate calibration-assisted high-performance liquid chromatography with dual UV and fluorimetric detection for the analysis of natural and synthetic sex hormones in environmental waters and sediments A green method is reported based on non-sophisticated instrumental for the quantification of seven n...
  2. Liquid chromatography with diode array detection and multivariate curve resolution for the selective and sensitive quantification of estrogens in natural waters. Following the green analytical chemistry principles, an efficient strategy involving second-order da...
  3. Deporte, cultura y sociedad: un estado actual de la cuestión Abstract: In the next article we set out to analyse the theoretical and empirical content of the lin...
  4. Mercado de trabajo en el deporte y género: un estudio comparativo entre las condiciones de trabajo de las tituladas y los titulados en Ciencias de la Actividad Física y el Deporte por las universidades catalanas Abstract: This paper aims to analyse the sources of employment for university graduates in Physical ...
  5. Deporte e Inclusión Social: aplicación del Programa de Responsabilidad Personal y Social en adolescentes Abstract:This research aims to establish the extent to which the Personal and Social Responsibility ...

Otros recursos de la mismacolección

  1. Understanding mechanisms to predict and optimize biochar for agrochemical sorption The ability of biochars to bind various organic compounds has been widely studied due to the potenti...
  2. RNA-seq of plant/pathogen interactions as a tool for deciphering effector genes Trabajo presentado en la 2nd Annual Conference of the SUSTAIN Action, celebrada en Zakopane (Poland)...
  3. Identifying allelic variants for papilla based powdery mildew adult plant resistance against powdery mildew in oat by association studies Trabajo presentado en la 2nd Annual Conference of the SUSTAIN Action, celebrada en Zakopane (Poland)...
  4. A functional approach to test the response of weed communities to agriculture intensification across two countries Trabajo presentado en el 17th European Weed Research Society Symposium (EWRS), celebrado en Montpel...
  5. Recruitment DsRed2-PKD1 (red channel) to the immune synapse is more obvious after deconvolution The two movies show a raw (1st movie) and Huygens deconvolved (2nd movie) image series of DsRed2-PKD...

Aviso de cookies: Usamos cookies propias y de terceros para mejorar nuestros servicios, para análisis estadístico y para mostrarle publicidad. Si continua navegando consideramos que acepta su uso en los términos establecidos en la Política de cookies.