1) La descarga del recurso depende de la página de origen
2) Para poder descargar el recurso, es necesario ser usuario registrado en Universia

Opción 1: Descargar recurso

Detalles del recurso


Dilatation of the ascending aorta (AAD) is a prevalent aortopathy that occurs frequently associated with bicuspid aortic valve (BAV), the most common human congenital cardiac malformation. The molecular mechanisms leading to AAD associated with BAV are still poorly understood. The search for differentially expressed genes in diseased tissue by quantitative real-time PCR (qPCR) is an invaluable tool to fill this gap. However, studies dedicated to identify reference genes necessary for normalization of mRNA expression in aortic tissue are scarce. In this report, we evaluate the qPCR expression of six candidate reference genes in tissue from the ascending aorta of 52 patients with a variety of clinical and demographic characteristics, normal and dilated aortas, and different morphologies of the aortic valve (normal aorta and normal valve n = 30; dilated aorta and normal valve n = 10; normal aorta and BAV n = 4; dilated aorta and BAV n = 8). The expression stability of the candidate reference genes was determined with three statistical algorithms, GeNorm, NormFinder and Bestkeeper. The expression analyses showed that the most stable genes for the three algorithms employed were CDKN1β, POLR2A and CASC3, independently of the structure of the aorta and the valve morphology. In conclusion, we propose the use of these three genes as reference genes for mRNA expression analysis in human ascending aorta. However, we suggest searching for specific reference genes when conducting qPCR experiments with new cohort of samples.

Pertenece a

Directory of Open Access Journals - Articles  


Carmen Rueda -  Martínez -  Oscar Lamas -  María José Mataró -  Juan Robledo -  Carmona -  Gemma Sánchez -  Espín -  Manuel Jiménez -  Navarro -  Miguel Such -  Borja Fernández - 

Id.: 65694842

Idioma: EN  - 

Versión: 1.0

Estado: Final

Palabras claveMedicine - 

Tipo de recurso: article  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí


Requerimientos técnicos:  Browser: Any - 

Relación: [IsBasedOn] PLoS ONE, Vol 9, Iss 5, p e97449 (2014)
[References] http://europepmc.org/articles/PMC4026239?pdf=render
[References] https://doaj.org/toc/1932-6203

Fecha de contribución: 16-jun-2016


* 10.1371/journal.pone.0097449
* 1932-6203

Otros recursos del mismo autor(es)

  1. Synthesis of 3,3-Disubstituted 2-Oxindoles by Deacylative Alkylation of 3-Acetyl-2-oxindoles An innovative and efficient monoalkylation and nonsymmetrical 3,3-dialkylation of oxindoles has been...
  2. Assisting non-expert speakers of under-resourced languages in assigning stems and inflectional paradigms to new word entries of morphological dictionaries This paper presents a new method with which to assist individuals with no background in linguistics ...
  3. The Chlamydomonas genome reveals the evolution of key animal and plant functions. Chlamydomonas reinhardtii is a unicellular green alga whose lineage diverged from land plants over 1...
  4. Singular FBSDEs and Scalar Conservation Laws Driven by Diffusion Processes Motivated by earlier work on the use of fully-coupled Forward-Backward Stochastic Differential Equat...
  5. Paris-Princeton Lectures on Mathematical Finance This is the fourth volume of the Paris-Princeton Lectures in Mathematical Finance. The goal of this ...

Otros recursos de la mismacolección

No existen otros recursos

Aviso de cookies: Usamos cookies propias y de terceros para mejorar nuestros servicios, para análisis estadístico y para mostrarle publicidad. Si continua navegando consideramos que acepta su uso en los términos establecidos en la Política de cookies.