Sunday, October 26, 2014

 

 



Soy un nuevo usuario

Olvidé mi contraseña

Entrada usuarios

Lógica Matemáticas Astronomía y Astrofísica Física Química Ciencias de la Vida
Ciencias de la Tierra y Espacio Ciencias Agrarias Ciencias Médicas Ciencias Tecnológicas Antropología Demografía
Ciencias Económicas Geografía Historia Ciencias Jurídicas y Derecho Lingüística Pedagogía
Ciencia Política Psicología Artes y Letras Sociología Ética Filosofía


Sequence-specific physical properties of African green monkey alpha-satellite DNA contribute to centromeric heterochromatin formation

1) La descarga del recurso depende de la página de origen
2) Para poder descargar el recurso, es necesario ser usuario
    registrado en Universia


  Descargar recurso

Detalles del recurso

Pertenece a: University of Twente Publications  

Descripción: Satellite DNA, a major component of eukaryotic centromeric heterochromatin, is potentially associated with the processes ensuring the faithful segregation of the genetic material during cell division. Structural properties of alpha-satellite DNA (AS) from African green monkey (AGM) were studied. Atomic force microscopy imaging showed smaller end-to-end distances of AS fragments than would be expected for the persistence length of random sequence DNA. The apparent persistence length of the AS was determined as 35 nm. Gel-electrophoresis indicated only a weak contribution of intrinsic curvature to the DNA conformations suggesting an additional contribution of an elevated bending flexibility to the reduced end-to-end distances. Next, the force-extension behavior of the naked AS and in complex with nucleosomes was studied using optical tweezers. The naked AS showed a reduced overstretching transition force (−18% the value determined for random DNA) and higher forces required to straighten the DNA. Finally, reconstituted AS nucleosomes disrupted at significantly higher forces as compared with random DNA nucleosomes which is probably due to structural properties of the AS which stabilize the nucleosomes. The data support that the AS plays a role in the formation of centromeric heterochromatin due to specific structural properties and suggest that a relatively higher mechanical stability of nucleosomes is important in AGM–AS chromatin.

Autor(es): Bussiek, Malte -  Hoischen, Christian -  Diekmann, Stephan -  Bennink, Martin L. - 

Id.: 55210785

Versión: 1.0

Estado: Final

Tipo:  application/pdf - 

Tipo de recurso: Article / Letter to editor  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

: © 2009 Elsevier

Formatos:  application/pdf - 

Requerimientos técnicos:  Browser: Any - 

Relación: [References] http://doc.utwente.nl/80073/1/Bussiek09sequence.pdf

Fecha de contribución: 21-abr-2012

Contacto:

Localización:


Otros recursos del mismo autor(es)

  1. A CENP-S/X complex assembles at the centromere in S and G2 phases of the human cell cycle The functional identity of centromeres arises from a set of specific nucleoprotein particle subunits...
  2. Cell-Cycle-Dependent Structural Transitions in the Human CENP-A Nucleosome In Vivo In eukaryotes, DNA is packaged into chromatin by canonical histone proteins. The specialized histone...
  3. Year Class Coexistence or Competitive Exclusion for Strict Biennials? We consider a discrete time model of semelparous biennial population dynamics. Interactions between ...
  4. Dynamics of CENP-N kinetochore binding during the cell cycle Accurate chromosome segregation requires the assembly of kinetochores, multiprotein complexes that a...
  5. Centromere anatomy in the multidrug-resistant pathogen Enterococcus faecium Multidrug-resistant variants of the opportunistic human pathogen Enterococcus have recently emerged ...

Otros recursos de la misma colección

  1. Ultra Small-angle X-ray Scattering Study of Flocculation in Silica-filled Rubber
  2. Deployment versus data retrieval costs for caches in the plane We consider the problem of finding the Pareto front of the expected deployment cost of wireless cach...
  3. Erbium-doped spiral amplifiers with 20 dB of net gain on silicon Spiral-waveguide amplifiers in erbium-doped aluminum oxide on a silicon wafer are fabricated and cha...
  4. Modulated scatterer technique probe for intra-body RF channel measurements For sensing human body parameters, several sensors (implantable or digestible) can be located inside...
  5. In-Situ measurement of high frequency emission caused by photo voltaic inverters Modern photovoltaic (PV) installations are being installed in large numbers. Manufacturers are using...

Valoración de los usuarios

No hay ninguna valoración para este recurso.Sea el primero en valorar este recurso.
 

Busque un recurso