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Human DNA polymerases mu (Polµ) and lambda (Polλ) are X family members involved in the repair of double-strand breaks in DNA during non-homologous end joining. Crucial abilities of these enzymes include bridging of the two 3′ single-stranded overhangs and trans-polymerization using one 3′ end as primer and the other as template, to minimize sequence loss. In this context, we have studied the importance of a previously uncharacterised sequence (‘brooch’), located at the N-terminal boundary of the Polß-like polymerase core, and formed by Tyr141, Ala142, Cys143, Gln144 and Arg145 in Polµ, and by Trp239, Val240, Cys241, Ala242 and Gln243 in Polλ. The brooch is potentially implicated in the maintenance of a closed conformation throughout the catalytic cycle, and our studies indicate that it could be a target of Cdk phosphorylation in Polµ. The brooch is irrelevant for 1 nt gap filling, but of specific importance during end joining: single mutations in the conserved residues reduced the formation of two ended synapses and strongly diminished the ability of Polµ and polymerase lambda to perform non-homologous end joining reactions in vitro.

Pertenece a

Repositorio Institucional de la Universidad de Cordoba :: Spain  


Martín, M.J. -  García -  Ortiz, M.V. -  Gómez -  Bedoya, Ana -  Esteban, Verónica -  Guerra, Susana -  Blanco, Luis - 

Id.: 70746449

Idioma: eng  - 

Versión: 1.0

Estado: Final

Tipo:  application/pdf - 

Palabras claveHuman DNA polymerase mu - 

Tipo de recurso: info:eu-repo/semantics/article  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

: http://creativecommons.org/licenses/by-nc-nd/3.0/es/

Formatos:  application/pdf - 

Requerimientos técnicos:  Browser: Any - 

Relación: [References] http://dx.doi.org/10.1093/nar/gkt681
[References] Gobierno de España. BFU2009-10085
[References] Gobierno de España. CSD2007-00015

Fecha de contribución: 16-dic-2017


* Nucleic Acids Research 41(19), 9105–9116 (2013)

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