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Metallointercalator−DNA conjugates were prepared by amide bond formation between active esters on the nonintercalating ligands of transition metal complexes and primary amines presented at the 5' or the 3' termini of oligonucleotides attached to solid supports. The conjugates were liberated from the support by aminolysis and purified by HPLC on C18 or C4 stationary phases, which separates the two diastereomeric forms of the conjugates containing either the Λ or the Δ enantiomer of the octahedral metal complex. The coupling reaction proceeds with ∼75% conversion of the amino-terminated oligonucleotide into the conjugate; the isolated yield is ∼200 nmol for syntheses initiated on DNA-synthesis columns with a loading of 2 μmol. The conjugates were characterized by ultraviolet−visible and circular dichorism absorption spectroscopy, electrospray ionization mass spectrometry, enzymatic digestion, and polyacrylamide gel electrophoresis (PAGE). Oligonucleotides bearing [Rh(phi)_2(bpy')]^(3+) (phi = 9,10-phenanthrene quinone diimine; bpy' = 4-butyric acid-4'-methyl bipyridyl) form 1:1 duplexes with the complementary strand, and the electrophoretic mobility under nondenaturating PAGE of duplexes containing Δ-Rh is notably different from duplexes containing Λ-Rh. High-resolution PAGE of DNA photocleavage reactions initiated by irradiation of the tethered Rh complexes reveal intercalation of the complex only near the tethered end of the duplex. Analogous DNA-binding properties were observed with [Rh(phi)_2(bpy')]^(3+) tethered to the 3' terminus. By combining the 3‘ and 5‘ modification strategies, a mixed-metal DNA conjugate containing both [Os(phen)(bpy')(Me_2-dppz)]^(2+) (Me_2-dppz = 7,8-dimethyldipyridophenazine) on the 3' terminus and [Rh(phi)_2(bpy')]^(3+) on the 5' terminus was prepared and isolated. Taken together, these strategies for preparing metallointercalator−DNA conjugates offer a useful approach to generate chemical assemblies to probe long-range DNA-mediated charge transfer where the redox initiator is confined to and intercalated in a well-defined binding site.

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Caltech Authors  


Holmlin, R. Erik -  Dandliker, Peter J. -  Barton, Jacqueline K. - 

Id.: 70962646

Versión: 1.0

Estado: Final

Tipo:  application/pdf - 

Tipo de recurso: Article  -  PeerReviewed  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

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Copyright: sí

Formatos:  application/pdf - 

Requerimientos técnicos:  Browser: Any - 

Relación: [References] http://resolver.caltech.edu/CaltechAUTHORS:20180202-151812038
[References] https://authors.library.caltech.edu/84658/

Fecha de contribución: 03-feb-2018


* Holmlin, R. Erik and Dandliker, Peter J. and Barton, Jacqueline K. (1999) Synthesis of Metallointercalator−DNA Conjugates on a Solid Support. Bioconjugate Chemistry, 10 (6). pp. 1122-1130. ISSN 1043-1802. http://resolver.caltech.edu/CaltechAUTHORS:20180202-151812038

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