1) La descarga del recurso depende de la página de origen
2) Para poder descargar el recurso, es necesario ser usuario registrado en Universia


Opción 1: Descargar recurso

Detalles del recurso

Descripción

Metallointercalator−DNA conjugates were prepared by amide bond formation between active esters on the nonintercalating ligands of transition metal complexes and primary amines presented at the 5' or the 3' termini of oligonucleotides attached to solid supports. The conjugates were liberated from the support by aminolysis and purified by HPLC on C18 or C4 stationary phases, which separates the two diastereomeric forms of the conjugates containing either the Λ or the Δ enantiomer of the octahedral metal complex. The coupling reaction proceeds with ∼75% conversion of the amino-terminated oligonucleotide into the conjugate; the isolated yield is ∼200 nmol for syntheses initiated on DNA-synthesis columns with a loading of 2 μmol. The conjugates were characterized by ultraviolet−visible and circular dichorism absorption spectroscopy, electrospray ionization mass spectrometry, enzymatic digestion, and polyacrylamide gel electrophoresis (PAGE). Oligonucleotides bearing [Rh(phi)_2(bpy')]^(3+) (phi = 9,10-phenanthrene quinone diimine; bpy' = 4-butyric acid-4'-methyl bipyridyl) form 1:1 duplexes with the complementary strand, and the electrophoretic mobility under nondenaturating PAGE of duplexes containing Δ-Rh is notably different from duplexes containing Λ-Rh. High-resolution PAGE of DNA photocleavage reactions initiated by irradiation of the tethered Rh complexes reveal intercalation of the complex only near the tethered end of the duplex. Analogous DNA-binding properties were observed with [Rh(phi)_2(bpy')]^(3+) tethered to the 3' terminus. By combining the 3‘ and 5‘ modification strategies, a mixed-metal DNA conjugate containing both [Os(phen)(bpy')(Me_2-dppz)]^(2+) (Me_2-dppz = 7,8-dimethyldipyridophenazine) on the 3' terminus and [Rh(phi)_2(bpy')]^(3+) on the 5' terminus was prepared and isolated. Taken together, these strategies for preparing metallointercalator−DNA conjugates offer a useful approach to generate chemical assemblies to probe long-range DNA-mediated charge transfer where the redox initiator is confined to and intercalated in a well-defined binding site.

Pertenece a

Caltech Authors  

Autor(es)

Holmlin, R. Erik -  Dandliker, Peter J. -  Barton, Jacqueline K. - 

Id.: 70962646

Versión: 1.0

Estado: Final

Tipo:  application/pdf - 

Tipo de recurso: Article  -  PeerReviewed  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

Formatos:  application/pdf - 

Requerimientos técnicos:  Browser: Any - 

Relación: [References] http://resolver.caltech.edu/CaltechAUTHORS:20180202-151812038
[References] https://authors.library.caltech.edu/84658/

Fecha de contribución: 03-feb-2018

Contacto:

Localización:
* Holmlin, R. Erik and Dandliker, Peter J. and Barton, Jacqueline K. (1999) Synthesis of Metallointercalator−DNA Conjugates on a Solid Support. Bioconjugate Chemistry, 10 (6). pp. 1122-1130. ISSN 1043-1802. http://resolver.caltech.edu/CaltechAUTHORS:20180202-151812038

Otros recursos del mismo autor(es)

  1. Structural Studies of Λ- and Δ-[Ru(phen)_2dppz]^(2+)Bound to d(GTCGAC)_2: Characterization of Enantioselective Intercalation ^1H and ^(31)P NMR have been applied in characterizing the enantioselective interactions between Λ- ...
  2. Probing Phenazine Electron Transfer and Retention in Pseudomonas Aeruginosa Biofilms The opportunistic pathogen Pseudomonas aeruginosa forms metabolically stratified biofilms. Redox-act...
  3. Long-Range Electron Transfer through DNA Films Regardless of its position within the DNA film, cross‐linked daunomycin (DM) is efficiently reduced ...
  4. NMR evidence for specific intercalation of Δ-rh(phen)_2phi^(3+) in [d(GTCGAC)_2] The anchoring of metal complexes in the major groove of DNA through intercalation has been increasin...
  5. A molecular light switch for DNA: Ru(bpy)_2(dppz)^(2+) [No abstract]

Otros recursos de la mismacolección

  1. Search for Tensor, Vector, and Scalar Polarizations in the Stochastic Gravitational-Wave Background The detection of gravitational waves with Advanced LIGO and Advanced Virgo has enabled novel tests o...
  2. The CARMA-NRO Orion Survey We present the first results from a new, high-resolution ^(12)CO(1–0), ^(13)CO(1–0), and C^(18)O(1–0...
  3. A wireless, low-drift, implantable intraocular pressure sensor with parylene-on-oil encapsulation This paper presents a wireless, implantable continuous intraocular pressure (IOP) monitoring system ...
  4. A 25Gb/s APD-based burst-mode optical receiver with 2.24ns reconfiguration time in 28nm CMOS This paper describes an avalanche photodetector (APD) based burst-mode optical receiver in 28nm CMOS...
  5. Charge carrier transport across grain boundaries in graphene We evaluate the charge carrier transmission across asymmetric grain boundaries (GB) in a graphene la...

Aviso de cookies: Usamos cookies propias y de terceros para mejorar nuestros servicios, para análisis estadístico y para mostrarle publicidad. Si continua navegando consideramos que acepta su uso en los términos establecidos en la Política de cookies.