1) La descarga del recurso depende de la página de origen
2) Para poder descargar el recurso, es necesario ser usuario registrado en Universia

Opción 1: Descargar recurso

Detalles del recurso


Objectives: Polyelectrolyte vesicles using layer-by-layer (LbL) were recently intro-duced for the encapsulation of therapeutic molecules. This work presents multilay-ered microcapsules of chitosan and a temperature-responsive elastin-like recom-binamer (ELR) as a novel drug delivery system. The release of a pre-loaded model protein was studied at distinct temperatures and number of layers to evaluate the permeability of these structures and their potential as tunable drug delivery devices. Methods: Sacrificial CaCO3 microparticles were prepared by co-precipitation of Na2CO3 and CaCl2 in a FITC-BSA solution under heavy stirring. LbL coating was performed by incubation with chitosan or ELR solutions, with a rinsing step in between. Capsules with 1, 3 and 5 bilayers were made. The CaCO3 cores were chelated using EDTA. The capsules were suspended in PBS at 25 and 37ºC and samples were taken every 24 hours for fluorescence measurements, during 14 days. Results: At both temperatures, cumulative release was higher for capsules with 1 bilayer, evidencing the role played by the capsules architecture in their perme-ability. The release kinetics among each temperature was also different: the BSA quantity released was higher at 25ºC than at 37ºC. Considering the case of a simple bilayer, in the former the cumulative release reaches 80%, while in the latter only 50% of the encapsulated protein is released. This result shows the effect of temperature in polyelectrolyte structures, namely when temperature-responsive materials like ELRs are used. Conclusions: Multilayered microcapsules based on chitosan and an ELR were studied as drug delivery vessels. Distinct release profiles of pre-loaded BSA at different temperatures and layer numbers demonstrated the influence of the capsules architecture and composition: more quantity of BSA is released for capsules with fewer layers and lower temperatures. These microcapsules have the potential for tunable drug release in tissue engineering applications by means of design changes.

Pertenece a

RepositóriUM Universidade do Minho  


Costa, Rui R. -  Arias, Francisco J. -  Rodríguez -  Cabello, José Carlos -  Mano, J. F. - 

Id.: 55298282

Idioma: eng  - 

Versión: 1.0

Estado: Final

Tipo de recurso: conferenceObject  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

: open access

Requerimientos técnicos:  Browser: Any - 

Relación: [References] www.artificial-organs.com

Fecha de contribución: 05-mar-2014


* 0391-3988

Otros recursos del mismo autor(es)

  1. What should be evaluated from a manager's perspective? Developing a salient protected area effectiveness evaluation system for managers and scientists in Spain The System for the Integrated Assessment of Protected Areas (SIAPA) was developed as a tool to impro...
  2. The σ1 Receptor Engages the Redox-Regulated HINT1 Protein to Bring Opioid Analgesia Under NMDA Receptor Negative Control © Copyright 2015, Mary Ann Liebert, Inc. Aims: The in vivo pharmacology of the sigma 1 receptor (σ1R...
  3. Proposal of a system for the integrated and comparative assessment of protected areas Appendix A: Supplementary data http://dx.doi.org/10.1016/j.ecolind.2012.05.009
  4. Results of the implementation of the System for the Integrated Assessment of Protected Areas (SIAPA) to the protected areas of the Autonomous Region of Madrid (Spain) Supplementary data: http://dx.doi.org/10.1016/j.ecolind.2013.04.019
  5. Limited uptake of protected area evaluation systems among managers and decision-makers in Spain and the Mediterranean Sea Supplementary material can be found online at http://dx.doi.org/10.1017/S0376892915000065

Otros recursos de la mismacolección

  1. Measuring software systems scalability for proactive data center management The current trend of increasingly larger Web-based applications makes scalability the key challenge ...
  2. A new plant modelling approach for formal verification purposes This paper presents a new approach in plant modeling for the formal verification of real time system...
  3. Refactoring smelly spreadsheet models Identifying bad design patterns in software is a successful and inspiring research trend. While thes...
  4. MDSheet - Model-Driven Spreadsheets This paper showcases MDSheet, a framework aimed at improving the engineering of spreadsheets. This f...
  5. Frictional contacts in multibody dynamics Dissertação de mestrado integrado em Engenharia Mecânica

Aviso de cookies: Usamos cookies propias y de terceros para mejorar nuestros servicios, para análisis estadístico y para mostrarle publicidad. Si continua navegando consideramos que acepta su uso en los términos establecidos en la Política de cookies.