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An important goal of structural studies of modular proteins is to determine the inter-module orientation, which often influences biological function. The N-terminal domain of human fibronectin (Fn) is composed of a string of five type I modules (F1). Despite their small size, to date F1 modules have proved intractable to X-ray structure solution, although there are several NMR structures available. Here, we present the first structures (two X-ray models and an NMR-derived model) of the (2)F1(3)F1 module pair, which forms part of the binding site for Fn-binding proteins from pathogenic bacteria. The crystallographic structure determination was aided by the novel technique of UV radiation damage-induced phasing. The individual module structures are very similar in all three models. In the NMR structure and one of the X-ray structures, a similar but smaller interdomain interface than that observed previously for (4)F1(5)F1 is seen. The other X-ray structure has a different interdomain orientation. This work underlines the benefits of combining X-ray and NMR data in the studies of multi-domain proteins. (c) 2007 Elsevier Ltd. All rights reserved.

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Rudino -  Pinera, Enrique -  Ravelli, Raimond B. G. -  Sheldrick, George M. -  Nanao, Max H. -  Korostelev, Vladimir V. -  Werner, Joern M. -  Schwarz -  Linek, Ulrich -  Potts, Jennifer R. -  Garman, Elspeth F. - 

Id.: 55464629

Versión: 1.0

Estado: Final

Tipo de recurso: Article  -  PeerReviewed  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

Requerimientos técnicos:  Browser: Any - 

Relación: [References] http://dx.doi.org/10.1016/j.jmb.2007.02.061
[References] http://eprints.whiterose.ac.uk/62391/

Fecha de contribución: 08-jun-2012


* Rudino-Pinera, Enrique, Ravelli, Raimond B. G., Sheldrick, George M., Nanao, Max H., Korostelev, Vladimir V., Werner, Joern M., Schwarz-Linek, Ulrich, Potts, Jennifer R. and Garman, Elspeth F. (2007) The solution and crystal structures of a module pair from the Staphylococcus aureus-binding site of human fibronectin - A tale with a twist. Journal of Molecular Biology . pp. 833-844. ISSN 0022-2836

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