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Variable and low egg quality is a major limiting factor for the development of efficient aquaculture production. This stems from limited knowledge on the mechanisms underlying egg quality in cultured fish. Molecular analyses, such as transcriptomic studies, are valuable tools to identify the most important processes modulating egg quality. However, very few studies have been devoted to this aspect so far. Within this study, the microarray-based transcriptomic analysis of eggs (of different quality) of sea bass (Dicentrarchus labrax) was performed. An Agilent oligo microarray experiment was performed on labelled mRNA extracted from 16 batches of eggs (each batch obtained from a different female) of sea bass, in which over 24,000 published probe arrays were used. We identified 39 differentially expressed genes exhibiting a differential expression between the groups of low (fertilization rate < 60 %) and high (fertilization rate > 60 %) quality. The mRNA levels of eight genes were further analyzed by quantitative PCR. Seven genes were confirmed by qPCR to be differentially expressed in eggs of low and high quality. This study confirmed the importance of some of the genes already reported to be potential molecular quality indicators (mainly rnf213 and irf7), but we also found new genes (mainly usp5, mem-prot, plec, cenpf), which had not yet been reported to be quality-dependent in fish. These results suggest the importance of genes involved in several important processes, such as protein ubiquitination, translation, DNA repair, and cell structure and architecture; these probably being the mechanisms that contribute to egg developmental competence in sea bass.

Pertenece a

ArchiMer, Institutional Archive of Ifremer (French Research Institute for Exploitation of the Sea)  

Autor(es)

Żarski, Daniel -  Nguyen, Thaovi -  Le Cam, Aurélie -  Montfort, Jérôme -  Dutto, Gilbert -  Vidal, Marie -  odile -  Fauvel, Christian -  Bobe, Julien - 

Id.: 69632786

Idioma: eng  - 

Versión: 1.0

Estado: Final

Tipo:  application/pdf - 

Palabras claveMicroarray - 

Tipo de recurso: Texto Narrativo  -  Publication  -  info:eu-repo/semantics/article  - 

Tipo de Interactividad: Expositivo

Nivel de Interactividad: muy bajo

Audiencia: Estudiante  -  Profesor  -  Autor  - 

Estructura: Atomic

Coste: no

Copyright: sí

: The Author(s) 2017. This article is published with open access at Springerlink.com

Formatos:  application/pdf - 

Requerimientos técnicos:  Browser: Any - 

Relación: [IsBasedOn] Marine Biotechnology (1436-2228) (Springer Nature), 2017-02-08 , Vol. 19 , N. 1 , P. 102-115

Fecha de contribución: 07-mar-2017

Contacto:

Localización:
* DOI:10.1007/s10126-017-9732-1

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