Repository of the University of Hasselt containing publications in the fields of statistics, computer science, information strategies and material from the Institute for behavioural sciences.
Dept. Medical Basic Sciences (MBW) - Physiology
Mostrando recursos 1 - 20 de 438
Comorbidity Significantly Affects Clinical Outcome After Cardiac Resynchronization Therapy Regardless of Ventricular Remodeling - Verbrugge, Frederik H.; Dupont, Matthias; Rivero-Ayerza, Maximo; De Vusser, Philippe; Van Herendael, Hugo; Vercammen, Jan; Jacobs, Linda; Verhaert, David; Vandervoort, Pieter; Tang, W. H. Wilson; Mullens, Wilfried
Background: The influence of comorbid conditions on ventricular remodeling, functional status, and clinical outcome after cardiac resynchronization therapy (CRT) is insufficiently elucidated. Methods and Results: The influence of different comorbid conditions on left ventricular remodeling, improvement in New York Heart Association (NYHA) functional class, hospitalizations for heart failure, and all-cause mortality after CRT implantation was analyzed in 172 consecutive patients (mean age 71 +/- 9 y), implanted from October 2008 to April 2011 in a single tertiary care hospital. During mean follow-up of 18 +/- 9 months, 21 patients died and 57 were admitted for heart failure. Left ventricular remodeling...
Long-lasting enhancement of GABA(A) receptor expression in newborn dentate granule cells after early-life febrile seizures - Swijsen, Ann; Brône, Bert; Rigo, Jean-Michel; Hoogland, Govert
Febrile seizures (FS) are the most common type of seizures in childhood and are suggested to play a role in the development of temporal lobe epilepsy (TLE). Animal studies demonstrated that experimental FS induce a long-lasting change in hippocampal excitability, resulting in enhanced seizure susceptibility. Hippocampal neurogenesis and altered ion channel expression have both been proposed as mechanisms underlying this decreased seizure threshold. The present study aimed to analyze whether dentate gyrus (DG) cells that were born after FS and matured for 8 weeks display an altered repertoire of ligand-gated ion channels. To this end, we applied an established model,...
Pharmacology of Perioperative Intraperitoneal and Intravenous Chemotherapy in Patients with Peritoneal Surface Malignancy - Van der Speeten, Kurt; Stuart, O. Anthony; Sugarbaker, Paul H.
Peritoneal surface malignancy is a common manifestation of intra-abdominal malignancies. Systemic chemotherapy offers no long-term survival and poor quality of life for these patients in their terminal stages of disease. By contrast, cytoreductive surgery combined with perioperative intraperitoneal and intravenous chemotherapy has resulted in encouraging clinical results. Further improvements should come from both clinical phase II/III studies and pharmacologic research. This article reviews the current pharmacologic data and controversies regarding the perioperative intraperitoneal and intravenous application of chemotherapy.
No additional reduction of mortality or heart failure events with intrathoracic impedance measurements in a context of telemonitoring with structural biofeedback - Grieten, L.; Verbrugge, F. H.; Storms, V.; Verhaert, D.; Rivero-Ayerza, M.; De Vusser, P.; Van Herendael, H.; Vercammen, J.; Jacobs, L.; Rondelez, K.; Mullens, W.; Vandervoort, P.
Our aim was to test if intra-thoracic impedance (ITI) measurements would improve short-term clinical outcome (i.e. all-cause mortality and heart failure hospitalizations) in patients with cardiac resynchronization therapy (CRT) who were followed in a telemonitoring program with structural biofeedback. Methods: CRT patients (n=200; age= 70 +/-14y) were included in a telemonitoring program at Ziekenhuis Oost-Limburg Genk, which comprised daily transmissions through a wireless system installed at their homes. All patients were followed for potential lead/device problems, episodes of anti-tachycardia pacing, device shocks, occurrence of arrhytmias, a decline in biventricular pacing percentage and/or a sudden drop in physical activity. Patients with...
Ensemble and single particle fluorimetric techniques in concerted action to study the diffusion and aggregation of the glycine receptor alpha 3 isoforms in the cell plasma membrane - Notelaers, Kristof; Smisdom, Nick; Rocha, Susana; Janssen, Daniel; Meier, Jochen C.; Rigo, Jean-Michel; Hofkens, Johan; Ameloot, Marcel
The spatio-temporal membrane behavior of glycine receptors (GlyRs) is known to be of influence on receptor homeostasis and functionality. In this work, an elaborate fluorimetric strategy was applied to study the GlyR alpha 3K and L isoforms. Previously established differential clustering, desensitization and synaptic localization of these isoforms imply that membrane behavior is crucial in determining GlyR alpha 3 physiology. Therefore diffusion and aggregation of homomeric alpha 3 isoform-containing GlyRs were studied in HEK 293 cells. A unique combination of multiple diffraction-limited ensemble average methods and subdiffraction single particle techniques was used in order to achieve an integrated view of...
The effect of histamine, a traumatic brain injury mediator, on the function of aquaporin-4 in rat brain in vivo - Smets, A.; Nuydens, R.; De Jongh, R.; Pintelon, I.; Thys, S.; Timmermans, J. P.; Rigo, J. M.; Meert, T.
The high levels of histamine in brain after traumatic brain injury and the observed influence of histamine on aquaporin-1 (AQP-1) localization in gastric cells, lead to the hypothesis that histamine could play a major role in the pathophysiology of brain edema An intracerebroventricular (ICV) histamine induced brain edema model was developed in rats. Infusion of concentrations >= 50 mM histamine resulted in a dose-dependent intracranial pressure (ICP) increase and death. Furthermore, histological experiments showed the internalization of membrane-expressed AQP4 after histamine challenge. The decreased fraction of functional membrane-associated AQP4 might result in an increased intracranial p[ressure. In this new experimental...
Active H(1)-receptors protect against the harmful effects of H(2)-receptors in a histamine induced brain edema model - De Jongh, R.; Smets, A.; Nuydens, R.; Rigo, J. M.; MEERT, Theo
Inspired by the high histamine levels in the brains of traumatic brain injury (TBI) patients and earlier in vitro results with histamine in a cell swelling model, a histamine induced brain ederna model was developed in rats. In this new experimental model, selective histamine agonists and antagonists were tested. An acute lethal effect is caused by H(1)-antagunism after intracerebroventricular (ICV) histamine infusion. Moreover H(2)-receptor activation results in toxic effects, which are reduced with H1-receptor stimulation. These data indicate that active H(1)-receptors protect against the toxic II,-effect. This shielding effect of H(1)-receptor activity is confirmed in several experiments. Therefore central penetrating...
Is there a functional role for KCNMA1 in the multiple sclerosis? - Vanheel, A.; Brone, B.; DANIELS, Ruth; Stinissen, P.; Noben, J. P.; Hellings, N.
Purpose/Objective: A more detailed insight into disease mechanisms of multiple sclerosis (MS) is crucial for the development of new and more effective theories. MS is a chronic inflammatory autoimmune disease of the central nervous system. The aim of this study is to identify novel disease associated proteins that are functionally involved in the MS brain pathology. Materials and methods: In a previous proteomics study, brainstem proteins were obtained from Lewis rats with MBP induced acute experimental autoimmune encephalomyelitis (EAE), a well characterized disease model of MS. Samples were collected at different time points: just before onset of symptoms, at the...
Impedance cardiography in uncomplicated pregnancy and pre-eclampsia: A reliability study - Tomsin, K.; Mesens, T.; Molenberghs, G.; Gyselaers, W.
It has been reported that cardiac contractility is altered in pre-eclampsia compared with normal pregnancy. Because of the non-invasive nature of impedance cardiography (ICG), this method is gaining popularity in the obstetric field. We assessed the reliability of ICG measurements in uncomplicated 3rd trimester pregnancies (UP) and pre-eclamptic pregnancies (PE). ICG measurements were recorded before and after three position changes, and this examination was done twice (session 1 and 2) per subject. For each of the 22 haemodynamic parameters, inter- and intrasession Pearson's correlation coefficients (PCC) were calculated for mean values of 30 measurements per position per subject. PCC was...
Microglia Proliferation Is Controlled by P2X7 Receptors in a Pannexin-1-Independent Manner during Early Embryonic Spinal Cord Invasion - Rigato, Chiara; Swinnen, Nina; Buckinx, Roeland; Couillin, Isabelle; Mangin, Jean-Marie; Rigo, Jean-Michel; Legendre, Pascal; Le Corronc, Herve
Microglia are known to invade the mammalian spinal cord (SC) at an early embryonic stage. While the mechanisms underlying this early colonization of the nervous system are still unknown, we recently found that it is associated, at least partially, with the ability of microglia to proliferate at the onset of motoneuron developmental cell death and of synaptogenesis in mouse embryo (E13.5). In vitro studies have shown that the proliferation and activation of adult microglia can be influenced by the purinergic ionotropic receptor P2X7 via a coupling with Pannexin-1. By performing patch-clamp recordings in situ using a whole-mouse embryonic SC preparation,...
Distributing the Optical Near-Field for Efficient Field-Enhancements in Nanostructures - Valev, V. K.; De Clercq, B.; Biris, C. G.; Zheng, X.; Vandendriessche, S.; Hojeij, M.; Denkova, D.; Jeyaram, Y.; Panoiu, N. C.; Ekinci, Y.; Silhanek, A. V.; Volskiy, V.; Vandenbosch, G. A. E.; Ameloot, M.; Moshchalkov, V. V.; Verbiest, T.
Circularly polarized light imparts a sense of rotation on the electron density in ring-shaped gold nanostructures. As a consequence, the near-field enhancement becomes homogeneous on the surface of the nanostructures, thereby increasing the opportunity for interaction with molecules. This type of nanostructured samples can find a broad range of applications in chemical processes where the interaction between molecules and local field enhancements play an important role.
Differential Response to Cardiac Resynchronization Therapy and Clinical Outcomes According to QRS Morphology and QRS Duration - Dupont, Matthias; Rickard, John; Baranowski, Bryan; Varma, Niraj; Dresing, Thomas; Gabi, Alaa; Finucan, Michael; Mullens, Wilfried; Wilkoff, Bruce L.; Tang, W. H. Wilson
Objectives The goal of this study was to examine the relative impact of QRS morphology and duration in echocardiographic responses to cardiac resynchronization therapy (CRT) and clinical outcomes. Background At least one-third of all patients treated with CRT fail to derive benefit. Patients without left bundle branch block (LBBB) or patients with smaller QRS duration (QRSd) respond less or not at all to CRT. Methods We retrospectively assessed baseline characteristics, clinical and echocardiographic response, and outcomes of all patients who received CRT at our institution between December 2003 and July 2007. Patients were stratified into 4 groups according to their...
Who should receive calcium and vitamin D supplementation? - Verbrugge, Frederik H.; Gielen, Evelien; Milisen, Koen; Boonen, Steven
Combined calcium and vitamin D supplementation is recommended in the prevention and treatment of osteoporosis. Until recently, supplementation was perceived as harmless without adverse effects. However, recent meta-analyses have provided evidence suggesting that calcium supplements, whether or not in combination with vitamin D, may be associated with cardiovascular risks. Although this finding constitutes a safety signal that has to be taken seriously, these data have to be interpreted with some caution. Current data do not allow definite conclusions to be drawn, but require further independent confirmation, since in numerous large studies, combined calcium and vitamin D supplementation did not increase...
Prolonged exercise training increases intramuscular lipid content and perilipin 2 expression in type 1 muscle fibres of patients with type 2 diabetes. - Shaw, C.S.; Shepherd, S.O.; Wagenmakers, A.J.M.; Hansen, D.; Dendale, P.; van Loon, L.J.C
The aim of the present study was to investigate changes in intramuscular triglyceride (IMTG) content and perilipin 2 expression in skeletal muscle tissue following 6 months of endurance type exercise training in type 2 diabetes patients, 10 obese, male type 2 diabetes patients (age 62 ??1 y, BMI 31??1 kg/m2) completed 3 exercise sessions per week, consisting of 40 min ofcontinuous endurance type exercise at 75% VO2 peak, for a period of 6 months. Muscle biopsies collected at baseline and after 2 and 6 months of intervention were analysed for IMTG content and perilipin 2 expression using fibre type specific...
The prognostic ability of pulmonary arterial capacitance exceeds that of pulmonary vascular resistance in advanced left sided heart failure - Dupont, Matthias; Mullens, Wilfried; Wu,Yuping; Tang, Wai Hong
Background: Right ventricular(RV) failure frequently occurs in left-sided heart failure(HF) and independently prognosticates. we investigated the determinants and prognostic role of the RV afterload measure pulmonary arterial capacitance (PAC), defined as the quotient of stroke volume and pulmonary arterial pulse pressure. Methods: We reviewed 724 HF patients who underwent right heart catheterization between 2000 and 2005. Survival was defined as freedom from all-cause mortality and transplantation. Results : In our patient cohort (age 55 ± 11 years, EF 19 ± 9 %), median PAC was 2.5 [1.60 - 3.96] mL/mmHg. PAC showed an inverse relation with pulmonary vascular resistance (PVR),...
Pericardial Disorders - Abrahams, Zuheir; Skouri, Hadi N.; Mullens, Wilfried; Yamani, Mohammad H.; Starling, Randall C.
Pharmacokinetics of the Perioperative Use of Cancer Chemotherapy in Peritoneal Surface Malignancy Patients - Van der Speeten, K.; Govaerts, K.; Stuart, O. A.; Sugarbaker, P. H.
Background. The peritoneal surface is an acknowledged locoregional failure site of abdominal malignancies. Previous treatment attempts with medical therapy alone did not result in long-term survival. During the last two decades, new treatment protocols combining cytoreductive surgery with perioperative intraperitoneal and intravenous cancer chemotherapy have demonstrated very encouraging clinical results. This paper aims to clarify the pharmacologic base underlying these treatment regimens. Materials and Methods. A review of the current pharmacologic data regarding these perioperative chemotherapy protocols was undertaken. Conclusions. There is a clear pharmacokinetic and pharmacodynamic rationale for perioperative intraperitoneal and intravenous cancer chemotherapy in peritoneal surface malignancy patients.
Identification of Protein Networks Involved in the Disease Course of Experimental Autoimmune Encephalomyelitis, an Animal Model of Multiple Sclerosis - Vanheel, Annelies; Daniels, Ruth; Plaisance, Stephane; Baeten, Kurt; Hendriks, Jerome J. A.; Leprince, Pierre; Dumont, Debora; Robben, Johan; Brone, Bert; Stinissen, Piet; Noben, Jean-Paul; Hellings, Niels
A more detailed insight into disease mechanisms of multiple sclerosis (MS) is crucial for the development of new and more effective therapies. MS is a chronic inflammatory autoimmune disease of the central nervous system. The aim of this study is to identify novel disease associated proteins involved in the development of inflammatory brain lesions, to help unravel underlying disease processes. Brainstem proteins were obtained from rats with MBP induced acute experimental autoimmune encephalomyelitis (EAE), a well characterized disease model of MS. Samples were collected at different time points: just before onset of symptoms, at the top of the disease and...
Neuromuscular electrical stimulation increases muscle protein synthesis in elderly, type 2 diabetic men. - Wall, Benjamin T.; Dirks, Marlou L.; Verdijk, Lex B.; Snijders, Tim; Hansen, Dominique; Vrankcx, Pascal; Burd, Nicolas A.; Dendale, Paul; van Loon, Luc J.C.
Physical activity is required to attenuate the loss of skeletal muscle with aging. Short periods of muscle disuse, due to sickness of hospitalization, reduce muscle protein synthesis rates, resulting in rapid muscle loss. The present study investigates the capacity of neuromuscular electrical stimulation (NMES) to increase in vivo skeletal muscle protein synthesis rates in older, type 2 diabetes patients. Six elderly, type 2 diabetic men (70??2 y) were subjected to 60 min of one-legged NMES. Continuous infusions with L-[ring-13C6] phenylalanine were applied, with blood and muscle samples being collected regularly to assess muscle protein synthesis rates in both the stimulated...