VAN STEENKISTE, Niels; TESSENS, Bart; WILLEMS, Wim; Van Mulken, Els; ARTOIS, Tom
Gieysztoria Ruebush and Hayes, 1939, the most species-rich genus of limnic rhabdocoels (Platyhelminthes: Dalyelliidae), is subdivided into several groups based on stylet morphology. Most taxa are known from the Palearctic and the Neotropics. Very little is known about limnic, free-living flatworms in other tropical and subtropical regions. In this contribution five new species of Gieysztoria are described from India, Australia and South Africa, all former Gondwanan fragments. Gieysztoria garudae n. sp., G. ashokae n. sp. and G. ramayana n. sp. were collected in Goa (India), G. stokesi n. sp. in the Northern Territory (Australia) and G. zuluensis n. sp. in...
De Broyer, Claude; Danis, Bruno; Allcock, Louise; Angel, Martin V.; Arango, Claudia; Artois, Tom; Barnes, D.; Bartsch, Ilse; Bester, Marthan; Blachowiak-Samolyk, Kasia; Blazewicz, Magda; Bohn, Jens; Brandt, Angelika; Brandao, Simone Nunes; David, Bruno; de Salas, Miguel; Eleaume, Marc; Emig, Christian; Fautin, Daphne; George, Kai-Horst; Gillan, David; Gooday, Andrew; Hopcroft, Russ; Jangoux, Michel; Janussen, Dorte; Koubbi, Philippe; Kouwenberg, Juliana; Kuklinski, Piotr; Ligowski, Ryszard; Lindsay, Dhugal; Linse, Katrin; Longshaw, Matt; Lopez-Gonzalez, Pablo; Martin, Patrick; Munilla, Tomas; Muhlenhardt-Siegel, Ute; Neuhaus, Birger; Norenburg, Jon; Ozouf-Costaz, Catherine; Pakhomov, Evgeny; Perrin, William; Petryashov, Victor; Pena-Cantero, Alvaro; Piatkowski, Uwe; Pierrot-Bults, Annelies; Rocka, Anna; Saiz-Salinas, Jose; Salvini-Plawen, Luitfried; Scarabino, Victor; Schiaparelli, Stefano; Schrodl, Michael; Schwabe, Enrico; Scott, Fiona; Sicinski, Jacek; Siegel, Volker; Smirnov, Igor; Thatje, Sven; Utevsky, Andrei; Vanreusel, Ann; Wiencke, Christian; Woehler, Eric; Zdzitowiecki, Krzysztof; Zeidler, Wolfgang
The IPY sister-projects CAML and SCAR-MarBIN provided a timely opportunity, a strong collaborative framework and an appropriate momentum to attempt assessing the "Known, Unknown and Unknowable" of Antarctic marine biodiversity. To allow assessing the known biodiversity, SCAR-MarBIN "Register of Antarctic Marine Species (RAMS) was complied and published by a panel of 64 taxonomic experts. Thanks to this outstanding expertise mobilized for the first time, an accurate list of more than 8100 valid species was compiled and up-to-date systematic classification comprising more than 16800 taxon names was established. This taxonomic information is progressively and systematically completed by species occurrence data, provided...
Breugelmans, Karin; Quintana Cardona, Josep; ARTOIS, Tom; Jordaens, Kurt; Backeljau, Thierry
In April 2009 two specimens of a terrestrial flatworm were collected from under a rock in an orchard at Ciutadella de Menorca on the easternmost Balearic island of Menorca (Spain). Their external morphology suggested that both specimens belonged to the invasive blue planarian Caenoplana coerulea, a species which is native to eastern Australia. Sequence data of a fragment of the mitochondrial cytochrome c oxidase subunit I (COI) and of the entire 18S ribosomal RNA confirm its identification. This is one of the first records of the species in Europe where it has only been found in one locality in the...
Pieters, Nicky; Plusquin, Michelle; Cox, Bianca; Kicinski, Michal; Vangronsveld, Jaco; Nawrot, Tim S.
Objective Studies on the association between short-term exposure to ambient air pollution and heart rate variability (HRV) suggest that particulate matter (PM) exposure is associated with reductions in measures of HRV, but there is heterogeneity in the nature and magnitude of this association between studies. The authors performed a meta-analysis to determine how consistent this association is. Data source The authors searched the Pubmed citation database and Web of Knowledge to identify studies on HRV and PM. Study selection Of the epidemiologic studies reviewed, 29 provided sufficient details to be considered. The meta-analysis included 18667 subjects recruited from the population...
Artois, Tom; Willems, Wim; Revis, Nathalie; Martens, Paul M.; Schockaert, Ernest
Five new species of Limipolycystis Schilke, 1970 are described and their morphology is compared to that of the only species known, L. curvitubo Schilke, 1970. L. sicilicula n. sp., L. falx n. sp., L friedae n. sp., L. libra n. sp. and L. wallbergi n. sp. all largely resemble L. curvitubo as to the internal anatomy. They all have a caudally-situated testis and, at least in those species for which good material is available, a seminal receptacle could be seen. This seminal receptacle is tubiform, connecting the bursa with the most proximal part of the oviduct, which is filled with...
Curini-Galletti, Marco; Artois, Tom; Delogu, Valentina; De Smet, Willem H.; Fontaneto, Diego; Jondelius, Ulf; Leasi, Francesca; Martinez, Alejandro; Meyer-Wachsmuth, Inga; Nilsson, Karin Sara; Tongiorgi, Paolo; Worsaae, Katrine; Todaro, M. Antonio
Background: Biogeographical and macroecological principles are derived from patterns of distribution in large organisms, whereas microscopic ones have often been considered uninteresting, because of their supposed wide distribution. Here, after reporting the results of an intensive faunistic survey of marine microscopic animals (meiofauna) in Northern Sardinia, we test for the effect of body size, dispersal ability, and habitat features on the patterns of distribution of several groups. Methodology/Principal Findings: As a dataset we use the results of a workshop held at La Maddalena (Sardinia, Italy) in September 2010, aimed at studying selected taxa of soft-bodied meiofauna (Acoela, Annelida, Gastrotricha, Nemertodermatida,...
Janssen, Bram; Munters, Elke; Pieters, Nicky; Smeets, Karen; Cox, Bianca; Cuypers, Ann; Fierens, Frans; Penders, Joris; Vangronsveld, Jaco; Gyselaers, Wilfried; Nawrot, Tim S.
BACKGROUND: Studies emphasize the importance of particulate matter (PM) in the
formation of reactive oxygen species and inflammation. We hypothesized that these
processes can influence mitochondrial function of the placenta and fetus.
OBJECTIVES: We investigated the influence of PM10 exposure during pregnancy on the
mitochondrial DNA6content (mtDNA content) of the placenta and umbilical cord blood.
METHODS: DNA was extracted from placental tissue (n = 174) and umbilical cord
leukocytes (n = 176). Relative mtDNA copy numbers (i.e. mtDNA content) were determined
by real6time PCR. Multiple regression models were used to link mtDNA content and in utero
exposure to PM10 over...
Plusquin, Michelle; Stevens, An-Sofie; Van Belleghem, Frank; Degheselle, Olivier; Van Roten, Andromeda; Vroonen, Jessica; Blust, Ronny; Cuypers, Ann; Artois, Tom; Smeets, Karen
The planarian Schmidtea mediterranea is a well-studied model organism for developmental research, because of its stem cell system. This characteristic also provides a unique opportunity to study stress management and the effect of stress on stem cells. In this study, we characterised the stress signature at different levels of biological organization. The carcinogenic metal cadmium was used as a model chemical stressor. We focused on stem cell activity and its interaction with other known stress parameters. Here, we have found that S. mediterranea is able to cope with high internal levels of cadmium. At endpoints such as size and mobility,...
Guedens, Wanda; Reygel, Patrick; Asperges, Michel
Fotosynthese-experimenten in het secundair onderwijs bleven tot nog toe beperkt tot het afdekken van Pelargoniumbladeren met aluminiumfolie en het ‘bellenexperiment’ met waterpest (Elodea canadensis)1. Het practicum ‘Microscopische waarnemingen van de fotosynthese’ laat echter toe de vorming van zuurstofgas en zetmeel op microscopische schaal te zien en bovendien wordt de kennis over (bio)chemie en biologie hierbij op een zeer hoog niveau van integreren gebracht.
NELISSEN, Katherine; Mulder, Monique; SMETS, Ilse; TIMMERMANS, Silke; SMEETS, Karen; AMELOOT, Marcel; Hendriks, Jerome J. A.
Cholesterol synthesis and transport in oligodendrocytes are essential for optimal myelination and remyelination in pathological conditions such as multiple sclerosis. However, little is known about cholesterol homeostasis in the myelin-forming oligodendrocytes. Liver X receptors (LXRs) are nuclear oxysterol receptors that regulate genes involved in cholesterol homeostasis and may therefore play an important role in de- and remyelination. We investigated whether LXRs regulate cholesterol homeostasis in oligodendrocytes. mRNA expression of genes encoding LXR-alpha and LXR-beta and their target genes (ABCA1, ABCG1, ABCG4, apoE, and LDLR) was detected in oligodendrocytes derived from both neonatal and adult rats using quantitative real-time PCR. The...
PLUSQUIN, Michelle; DEGHESELLE, Olivier; CUYPERS, Ann; GEERDENS, Ellen; VAN ROTEN, Andromeda; ARTOIS, Tom; SMEETS, Karen
The ﬂatworm species Schmidtea mediterranea
and Macrostomum lignano have become new and innovative model organisms in stem cell, regeneration and tissue
homeostasis research. Because of their unique stem cell
system, (lab) technical advantages and their phylogenetic
position within the Metazoa, they are also ideal candidate
model organisms for toxicity assays. As stress and biomarker screenings are often performed at the transcriptional level, the aim of this study was to establish a set of
reference genes for qPCR experiments for these two model
organisms in different stress situations. We examined the
transcriptional stability of nine potential reference genes
(actb, tubb, ck2, cox4, cys, rpl13, gapdh, gm2ap, plscr1) to
assess those that...
Reygel, Patrick; Asperges, Michel; Guedens, Wanda
Deze bijdrage bevat materiaal voor de organisatie van een uitgebreid seminarie in het domein van de vakdidactiek van de Biologie/Natuurwetenschappen. De beschreven workshop en alle experimenten werden in de loop van meerdere jaren getest en gebruikt in een academische lerarenopleiding in het kader van het opleidingsonderdeel Vakdidactiek Biologie/Chemie aan de UHasselt. Het seminarie of een selectie ervan kan ook gebruikt worden in professionele bacheloropleidingen en mits tekstaanpassingen in het Secundair Onderwijs en dit zowel binnen het meeruren pakket Biologie als Natuurwetenschappen.
Asperges, Michel; Guedens, Wanda; Reygel, Patrick
Vakdidactiek en -methodiek zijn bij vakleerkrachten niet erg in trek. Het is zelfs moeilijk om recente vakliteratuur voor Vlaanderen over dit onderwerp te vinden. Rond het jaar 1974 startten, op aandringen van Rijksinspectrice Dr. M. De Ridder en zuster Dr. M. Van Dijck, enkele docenten uit het toenmalige regentaat biologie netoverstijgende vergaderingen rond een mogelijke cursus didactiek en methodologie biologie. Dit zou in 1979 resulteren in een handboek. Vooral Deconinck W. (Asperges M. e.a., 1979) werkte inspirerend. Biologie als schoolvak zou van een louter kennisvak - gericht op het kunnen reproduceren van weetjes - veranderen in een wetenschappelijk verantwoord vak...
Guedens, Wanda; Reygel, Patrick; Asperges, Michel
Fotosynthese-experimenten in het secundair onderwijs bleven tot nog toe beperkt tot het afdekken
van pelargoniumbladeren met aluminiumfolie en het ‘bellenexperiment’ met waterpest (Elodea
canadensis). Het practicum microscopische waarnemingen van de fotosynthese laat echter toe de
vorming van zuurstofgas en zetmeel op microscopische schaal te zien en bovendien wordt de kennis
over (bio)chemie en biologie hierbij op een zeer hoog niveau van integreren gebracht.
Weyens, Nele; Boulet, Jana; Adriaensen, Kristin; Timmermans, Jean-Pierre; Prinsen, Els; Van Oevelen, Sandra; D'Haen, Jan; Smeets, Karen; Van Der Lelie, Daniel; Taghavi, Safiyh; Vangronsveld, Jaco
Abstract This study aims to investigate the colonization of poplar by the endophyte Pseudomonas
putida W619 and its capacity to promote plant
growth. Poplar cuttings were inoculated with P.
putida W619 (wild-type or gfp-labelled). The colonization of both strains was investigated and morphological, physiological and biochemical parameters
were analyzed to evaluate plant growth promotion.
Inoculation with P. putida W619 (wild-type) resulted
in remarkable growth promotion, decreased activities
of antioxidative defence related enzymes, and reduced
stomatal resistance, all indicative of improved plant
health and growth in comparison with the noninoculated cuttings. In contrast, inoculation with gfplabelled P. putida W619 did not promote growth; it
even had a negative effect on plant...
Ruttens, Ann; Boulet, Jana; Weyens, Nele; Smeets, Karen; Adriaensen, Kristin; Meers, Erik; Van Slycken, Stijn; Tack, F.; Meiresonne, Linda; Thewys, Theo; Witters, Nele; Carleer, Robert; Dupae, Joke; Vangronsveld, Jaco
Phytoremediation, more precisely phytoextraction, has been placed forward as an environmental friendly remediation technique, that can gradually reduce increased soil metal concentrations, in particular the bioavailable fractions. The aim of this study was to investigate the possibilities of growing willows and poplars under short rotation coppice (SRC) on an acid, poor, sandy metal contaminated soil, to combine in this way soil remediation by phytoextraction on one hand, and production of biomass for energy purposes on the other. Above ground biomass productivities were low for poplars to moderate for willows, which was not surprising, taking into account the soil conditions that...
Janssen, Bram; Pieters, Nicky; Munters, Elke; Smeets, Karen; Cuypers, Ann; Penders, Joris; Vangronsveld, Jaco; Gyselaers, Wilfried; Nawrot, Tim
Recent studies have emphasized the importance of PM, and its associated metal components, in the formation of reactive oxygen species (ROS) and inflammation. Mitochondria are the major intracellular sources and primary targets of ROS. Mitochondrial DNA (mtDNA) is particularly vulnerable to ROS-induced damage, resulting in a higher mutation rate that impacts mitochondrial function. Given its multiple essential roles in metabolic pathways, mitochondrial dysfunction (i.e. change in mtDNA-content) is important in fetal development. In utero exposure to environmental factors may influence mitochondrial function which may comprise a mechanism of earlier onset of multifactorial disease such as cardiovascular disease in later life1.
Boone, Mieke; Bert, Wim; Claeys, Myriam; Houthoofd, Wouter; Artois, Tom
The ultrastructure of the testes in two representatives of the enigmatic taxon Nemertodermatida was studied using transmission electron microscopy. Nemertoderma westbladi has paired testes, which are delineated by lining cells. Within each testis, different follicles, each surrounded by a membrane-like structure, are found. Flagellophora apelti has genuinely follicular testes, consisting of several follicles, each containing a certain stage of spermatogenesis. As the gametes are not enclosed by a structure that can be called a true gonad, the structure of the testes differs from most bilaterian animals, but resembles the organization of gametogenic areas of ctenophores. Each stage of spermatogenesis in...
Boone, Mieke; HOUTHOOFD, Wouter; Bert, Wim; ARTOIS, Tom
This research was funded by an aspirant grant to MB provided by the Research Foundation Flanders (FWO). The authors kindly thank the Flemish Marine Institute (VLIZ), especially Andre Cattrijse, for the use of the research vessel "Zeeleeuw", and would like to acknowledge the help of Nathalie De Hauwere (VLIZ) with the map of the sampling points. The sampling campaigns fit within project G.08.208 financed by the Research Foundation-Flanders (FWO-Vlaanderen).
VAN STEENKISTE, Niels; Gobert, Stefan; Davison, Paul; Kolasa, Jurek; ARTOIS, Tom
Nine taxa of Dalyelliidae Graff, 1908 collected from limnic habitats in Ontario, Canada and Michigan and Alabama, USA are discussed. Two taxa are new to science. Pseudodalyellia alabamensis n. gen. n. sp. has a unique combination of characters (Microdalyellia-type stylet with two proximal heteromorphic axes of different size and two distal axes with long spines flanking a central spiny plate, uterus without uterine sphincter, glandular protrusion in male atrium) and is therefore placed in a new genus. Gieysztoria choctaw n. sp. is strikingly similar to the Amazonian G. chiqchi Damborenea et al., 2005 but differs by having 13 symmetrically arranged,...