Document Server@UHasselt
(3.246 recursos)
Repository of the University of Hasselt containing publications in the fields of statistics, computer science, information strategies and material from the Institute for behavioural sciences.
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3-D apple skin autofluorescence studied with two-photon excitation microscopy - VAN DE VEN, Martin; deGrauw, CJ; HUYBRECHTS, Christy; CISCATO, Massimo; Sowinska, M; Heisel, F; Gerritsen, HC; Deckers, T; AMELOOT, Marcel; VALCKE, Roland
Limburgs Univ Ctr, B-3590 Diepenbeek, Belgium. Univ Utrecht, NL-3584 CC Utrecht, Netherlands. CNRS, F-67037 Strasbourg, France. Royal Res Stn, B-3800 Sint Truiden, Belgium.
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Diffusion of myelin oligodendrocyte glycoprotein in living OLN-93 cells investigated by raster-scanning image correlation spectroscopy (RICS) - GIELEN, Ellen; SMISDOM, Nick; DE CLERCQ, Ben; VAN DE VEN, Martin; Gijsbers, Rik; Debyser, Zeger; RIGO, Jean-Michel; Hofkens, Johan; Engelborghs, Yves; AMELOOT, Marcel
Many membrane proteins and lipids are partially confined in substructures ranging from tens of nanometers to micrometers in size. Evidence for heterogeneities in the membrane of oligodendrocytes, i.e. the myelin-producing cells of the central nervous system, is almost exclusively based on detergent methods. However, as application of detergents can alter the membrane phase behaviour, it is important to investigate membrane heterogeneities in living cells. Here, we report on the first investigations of the diffusion behavior of the myelin-specific protein MOG (myelin oligodendrocyte glycoprotein) in OLN-93 as studied by the recently developed RICS (raster-scanning image correlation spectroscopy) technique. We implemented RICS...
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Chinese hamster ovary cell viability on hydrogen and oxygen terminated nano- and microcrystalline diamond surfaces - SMISDOM, Nick; SMETS, Ilse; WILLIAMS, Oliver; DAENEN, Michael; WENMACKERS, Sylvia; HAENEN, Ken; NESLADEK, Milos; D'HAEN, Jan; WAGNER, Patrick; RIGO, Jean-Michel; AMELOOT, Marcel; VAN DE VEN, Martin
Transfected Chinese hamster ovary cells were cultured on
bare uncoated chemical vapor deposited thin nano- and
microcrystalline diamond surfaces, hydrophobic hydrogenand
hydrophilic oxygen-terminated. Optical and biochemical
analyses show that compared to glass controls, growth and viability were not significantly affected (one-way-analysis of
variance, ANOVA). Based on two-way ANOVA analyses,
neither grain size nor surface termination had a significant
influence until 5 days post-seeding.
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Structural and optical properties of DNA layers covalently attached to diamond surfaces - WENMACKERS, Sylvia; POP, SD; Roodenko, K; VERMEEREN, Veronique; WILLIAMS, Oliver; DAENEN, Michael; DOUHERET, Olivier; D'HAEN, Jan; HARDY, An; VAN BAEL, Marlies; Hinrichs, K.; Cobet, C; VAN DE VEN, Martin; AMELOOT, Marcel; HAENEN, Ken; MICHIELS, Luc; Esser, N.; WAGNER, Patrick
Label-free detection of DNA molecules on chemically vapor-deposited diamond surfaces is achieved with spectroscopic ellipsometry in the infrared and vacuum ultraviolet range. This nondestructive method has the potential to yield information on the average orientation of single as well as double-stranded DNA molecules, without restricting the strand length to the persistence length. The orientational analysis based on electronic excitations in combination with information from layer thicknesses provides a deeper understanding of biological layers on diamond. The pi-pi* transition dipole moments, corresponding to a transition at 4.74 eV, originate from the individual bases. They are in a plane perpendicular to the...
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Synthetic diamond films as a platform material for label-free protein sensors - BIJNENS, Nathalie; VERMEEREN, Veronique; DAENEN, Michael; GRIETEN, Lars; HAENEN, Ken; WENMACKERS, Sylvia; WILLIAMS, Oliver; AMELOOT, Marcel; VAN DE VEN, Martin; MICHIELS, Luc; WAGNER, Patrick
In the framework of developing a fast and label-free immunosensor for C-reactive protein (CRP) detection, H-terminated nanocrystalline diamond (NCD) was functionalised with anti-CRP antibodies that were physically adsorbed to the surface. Impedance spectroscopy was used to electronically detect real-time CRP recognition. Different impedance behaviours were observed after CRP addition as compared to after FITC-labelled ssDNA addition at low (100 Hz) as well as at high frequencies (1 MHz). Physical interpretations of the observed impedance changes were obtained by fitting the data to an equivalent electrical circuit. Concentrations of 1 mu M CRP were recognised with a reaction time of 30...
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Diamond-based DNA sensors: surface functionalization and read-out strategies - WENMACKERS, Sylvia; VERMEEREN, Veronique; VAN DE VEN, Martin; AMELOOT, Marcel; BIJNENS, Nathalie; HAENEN, Ken; MICHIELS, Luc; WAGNER, Patrick
This article reviews the Current state-of-the art of diamond-based DNA sensors. Some general concepts involved in biosensors are introduced and applied to DNA sensors. The properties of chemically vapor deposited (CVD) diamond relevant for this application are summed up, with special attention for the stability and bio-compatibility of the material. Several routes to functionalize the diamond Surface are considered. The physical properties of the obtained DNA layers are discussed in terms of surface density and molecular conformation. Possible read-out strategies are evaluated, including optical and electronic sensing. With diamond-based DNA sensors, real-time and label-free sensing is achieved.
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