1.
Controllable fabrication of SnO2-coated nanotubes by chemical vapor multiwalled carbon deposition - Kuang Q; Li SF; Xie ZX; Lin SC; Zhang XH; Xie XY; Huang RB; Zheng LS
A simple and efficient approach for coating multiwalled carbon nanotubes (MWCNTs) with size-controllable SnO2 nanoparticles by chemical vapor deposition has been developed using tin hydride (SnH4) gas as the source of SnO2 at 550 degrees C. The size and coverage of SnO2 nanoparticles can be adjusted by simply controlling the deposition time and the flow rate of the SnH4/N-2 mixture gas during the CVD procedure. In addition, by using the MWCNTs as a sacrificial template, a kind of one-dimensional chain-like SnO2 nanostructure has been synthesized by increasing the deposition temperature to 730 degrees C. This technique may provide a good...
2.
Distinct roles of basal steady-state and induced H-ferritin in tumor necrosis factor-induced death in L929 cells - Xie Cc; Zhang N; Zhou HM; Li JQ; Li OX; Zarubin T; Lin SC; Han JH
Tumor necrosis factor (TNF) alpha is a cytokine capable of inducing caspase-dependent (apoptotic) cell death in some cells and caspase-independent (necrosis-like) cell death in others. Here, using a mutagenesis screen for genes critical in TNF-induced death in L929 cells, we have found that H-ferritin deficiency is responsible for TNF resistance in a mutant line and that, upon treatment with TNF, this line fails to elevate levels of labile iron pool (LIP), critical for TNF-induced reactive oxygen species (ROS) production and ROS-dependent cell death. Since we found that TNF-induced LIP in L929 cells is primarily furnished by intracellular storage iron, the...
3.
In situ step-scan time-resolved microscope FTIR spectroscopy working with a thin-layer cell - Zhou ZY; Lin SC; Chen SP; Sun SG
We report the development of an electrochemical in situ step-scan time-resolved microscope FTIR reflection spectroscopy (in situ SSTR-MFTIRS), which consists of a FTIR spectrometer with step-scan facility, an infrared microscope, a home-made signal synchronizer and a thin-layer IR cell working with a microelectrode. The employment of microelectrode has overcome successfully the inherent disadvantages of thin-layer IR cell in transient response. The time constant of the thin layer IR cell can be reduced to 37 mu s and the time resolution of IR spectra can reach as fast as 10 mu s. The results illustrated that the in situ SSTR-MFTIRS is...
4.
Axin contains three separable domains that confer intramolecular, homodimeric, and heterodimeric interactions involved in distinct functions - Luo W; Zou HY; Jin LH; Lin SY; Li QX; Ye ZY; Rui HL; Lin SC
Axin is a major scaffold protein, interacting with diverse molecules involved in a number of signaling pathways. Axin can undergo dimer/oligomerization via its DIX domain. Here we show that whereas deletion of the DIX domain at the C terminus rendered Axin incapable of forming dimer, a larger deletion of the C-terminal region restored the ability of Axin to form dimers. Detailed analyses revealed that Axin actually contains two separate domains (D and 1) in addition to the DIX domain for homodimerization. The D,I, and DIX domains alone can form homodimers. Interestingly, D and I domains strongly interact with each other,...
5.
RXR alpha acts as a carrier for TR3 nuclear export in a 9-cis retinoic acid-dependent manner in gastric cancer cells - Lin XF; ZHao BX; CHen XZ; Ye XF; Yang CY; ZHou HY; ZHang MQ; Lin SC; Wu Q
Retinoid X receptor (RXR) plays a crucial role in the cross talk between retinoid receptors and other hormone receptors including the orphan receptor TR3, forming different heterodimers that transduce diverse steroid/thyroid hormone signaling. Here we show that RXRalpha exhibits nucleocytoplasmic shuttling in MGC80-3 gastric cancer cells and that RXRalpha, shuttling is energy-dependent through a nuclear pore complex (NPC)mediated pathway for its import and an intact DNA binding domain-mediated pathway for its export. In the presence of its ligand 9-cis retinoic acid, RXRalpha was almost exclusively located in the cytoplasm. More importantly, we also show that RXRalpha. acts as a carrier...
6.
凡纳滨对虾G蛋白Gβ_1亚基的克隆、表达与鉴定 - 金利华; 骆晶晶; 林圣彩; 叶志云
根据G蛋白Gβ1亚基的保守序列设计简并引物,通过简并PCR和RACE技术,克隆到凡纳滨对虾(Litopenaeusvannamei)Gβ1基因的全长cDNA序列。利用Blast、DNAstar和Genedoc软件分析,发现该Gβ1编码的蛋白序列与其他物种已知的Gβ1序列具有相当高的保守性,将它命名为pvGβ1。免疫共沉淀分析发现pvGβ1能在体外适当条件下与对虾Gαs或Gαq相互结合。Westernblot-ting分析发现,pvGβ1在对虾身体各部位都有广泛分布,尤其在脑神经、眼和眼柄有大量表达。说明了Gβ1在对虾的神经系统和光信号传导等生命过程中的重要性。
【英文摘要】 The β/γ-subunits of the heterotrimeric GTP-binding proteins are important regulators of G-protein alpha subunits as well as a series of signal transduction receptors and effectors. In this study, a novel G protein β_1 subunit was isolated from shrimp Litopenaeus vannamei, and was termed pvGβ_1. Sequence analysis showed that the pvGβ_1 protein contained all the well-conserved domains and motifs that were critical sites for interaction with receptors and other binding effectors. Co-immunoprecipitation assay d...
7.
外源性RGS16基因稳定转染对胶质瘤C6细胞生长的影响 - 洪柳(第四军医大学西京医院病理科); 李青; 陈广生; 张丰; 聂蕾; 林圣彩
背景与目的:有研究表明野生型p53可以诱导RGS16表达,而RGS16可能与胶质瘤的发生有关。本研究旨在探讨RGS16基因转染对大鼠胶质瘤C6细胞生长的影响。方法:构建真核表达载体pIRES2-EGFP-RGS16,脂质体法转染C6细胞,经G418筛选后荧光显微镜观察细胞中增强型绿色荧光蛋白(enhancedgreenfluorscentprotein,EGFP)的表达,RT-PCR证实RGS16的mRNA表达,免疫细胞化学方法检测细胞中RGS16蛋白表达。最后利用流式细胞仪、生长曲线、平板克隆形成等方法研究RGS16基因对胶质瘤细胞周期、生长及增殖的影响。结果:成功构建了稳定表达RGS16和表达空载体的细胞系C6-RGS16、C6-GFP。C6-RGS16、C6-GFP和C6经流式细胞仪检测S期的细胞比例分别为28.5%、18.9%和14.3%(P<0.05);生长曲线表明C6-RGS16生长的速度明显快于C6-GFP及C6,但其平板克隆形成率分别为12%、25%和25%(P<0.05)。结论:RGS16促进C6细胞周期从G1期向S期过渡,加快细胞生长速度,但是并不促进细胞克隆形成。
【英文摘要】 BACKGROUND & OBJECTIVE: Wild type p53 could induceRGS16 mRNA expression, and RGS16 protein is related with carcinogenesisof glioma. This study was designed to investigate the effects of exogenousRGS16 gene transfection on the growth of rat glioma cell line C6.METHODS : A eukaryotic expression plasmid pIRES2-EGFP-RGS16 wasconstructed, and transfected into C6 cells. The cells were selected by G418.The expression of enhanced green fluorescent protein (EGFP) was observedunder fluorescent microscope, and the exp...
8.
RGS16与p53在人胶质瘤中的表达及相关性研究 - 洪柳(第四军医大学西京医院病理科); 李青; 陈广生; 张丰; 聂蕾; 张丽英; 林圣彩
目的研究G蛋白信号调节子16(RGS16)与p53在人胶质瘤中表达及其相关性。方法利用免疫组织化学链霉亲合素生物素过氧化物酶复合物(SABC)法检测42例胶质瘤标本中RGS16与p53的表达。结果RGS16在10例胶质瘤旁正常脑组织有8例神经元阳性但胶质细胞阴性、42例胶质瘤中37例肿瘤细胞阳性,二者差异显著(P<0.05);p53在瘤旁正常脑组织中阴性,胶质瘤中15例阳性,差异明显(P<0.05);RGS16、p53表达均与病理分型、分级无关(P>0.05);其中,RGS16与p53共同阳性表达11例,共同阴性表达1例,Kappa检验二者表达呈负相一致(P<0.05)。结论RGS16在胶质瘤中高表达而与病理分级无关,推测RGS16可能在胶质瘤发生中起作用。另外,胶质瘤中RGS16与p53表达呈负相关。
【英文摘要】 Objective To study the expression of RGS16 in human glioma and its relationship with p53. Methods The expression of RGS16 and p53 in protein level was studied by immunohistochemistry strept avidin-biotion-peroxidase-complex (SABC) method in 42 samples.Results In 10 normal brain tissues beside the human glioma, 8 cases expressed RGS16 in the neurons,but none in gliocytes; in 42 human gliomas, 37 cases expressed RGS16 in the glioma cells, and the difference between the normal tissue and the glioma was observe...
9.
胶质瘤中Axin基因点突变检测及表达研究 - 邵秋杰(第四军医大学西京医院病理科); 李青; 金利华; 赵一岭; 张英华; 林圣
目的 检测胶质瘤中Axin基因的点突变及其表达情况 ,初步探讨Axin与胶质瘤发生的关系。方法 采用聚合酶链反应 单链构象多态性 (PCR SSCP)技术及DNA测序方法检测Axin基因外显子 8,9及 10在 2 8例胶质瘤中的突变情况 ;同时对上述胶质瘤及正常脑组织进行免疫组化染色。结果 在 2 8例胶质瘤组织中Axin的第 10个外显子共有 6例样本 (2 1.4 % ) 3处发生了错义突变 ;3例 3处发生了同义突变 ;2 8例胶质瘤中 8例 (2 8.6 % )Axin表达阳性 ,正常脑组织中神经元表达阳性 ,神经胶质细胞表达阴性 ,检测到突变的样本中 1例表达阳性。结论 Axin基因的点突变可能参与胶质瘤的发生
【英文摘要】 Objective To detect the point mutations of Axin gene and its expression in glioma and explore the relationship between Axin gene and the occurrence of human glioma.Methods The point mutations of exon 8,9,10 of Axin gene were analyzed in 28 cases of glioma by polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) analysis, silver staining and DNA sequencing. Immunohistochemistry was used to detect the Axin expression in these cases...
10.
转染热休克蛋白70对p38MAPK信号通路的影响 - 王莉(第四军医大学西京医院病理科); 李青; 张丰; 郭斌; 郭爱林; 叶菁; 林圣彩; 樊代明
目的:探讨热休克蛋白 (HSP70 )在人胶质瘤细胞BT 32 5 p38MAPK信号通路中的作用。方法:用脂质体介导法将hsp70基因导入人胶质瘤细胞BT 32 5中 ,倒置显微镜观察转染细胞的形态学及粘附性变化 ,紫外线照射 30min后 ,采用免疫组化和Western blot方法测定转染前后HSP70的表达水平及照射前后 p38MAPK表达情况。结果:免疫组化和Western blot证实hsp70基因成功转染入BT 32 5中 ,转染细胞受到紫外线照射后 p38MAPK表达减弱。结论:体外转染hsp70基因可抑制紫外线照射后BT 32 5细胞 p38MAPK的表达.
Objective To study the role of HSP70 in p38MAPK signal transduction of human glioma cells BT 325.Methods pBBS212 hsp70 gene was transfected into BT 325 cells by lipofectin. The morphological and adhesive changes of the cells were observed under an inverted microscope. The level of HSP70 was measured by immunohistochemistry. Then the transfected cells were put into ultraviolet (UV) for 30 minitues, and expression of p38MAPK and HSP70 were examined by immunohistochemistry and Western blot methods bo...
11.
UV通过p38 MAPK促进大鼠胶质瘤C6细胞凋亡 - 张丰(第四军医大学西京医院病理科); 李青(第四军医大学基础部免疫学教研室); 王莉; 曹云新; 林圣彩
目的 :探讨 p38MAPK在紫外线损伤刺激细胞中的特异性信号转导作用 .方法 :流式细胞仪检测紫外线照射 30min后 1,2及 4h的C6细胞周期变化和是否有凋亡发生 ;应用免疫细胞化学技术观察紫外线刺激前后 p38MAPK在C6细胞中的表达强度和分布特征 .结果 :细胞周期结果显示 1,2和 4h后G1期细胞数分数各增多 0 .12 ,0 .2 1和 0 .19,而S期细胞数分数减少 0 .10 ,0 .14和 0 .15 ;各组的凋亡率分别是12 % ,4 9%和 34% ;未受刺激的细胞中 ,p38MAPK在胞质和胞核表达较弱 ;紫外线损伤作用 2h后 ,细胞核区的染色强度即明显增强 ,而胞质区域的染色强度相对降低 .结论 :C6细胞受紫外线损伤后可通过 p38MAPK通路发生凋亡.
【英文摘要】 AIM: To study the signal transduction of p38 mitogen activated protein kinase in rat glioma C6 cells after the stimulation of UV radiation. METHODS: Flow cytometry was applied to measure the fraction number changes in the cell cycle phase and to detect whether UV could induce apoptosis of C6 cells. The level and distribution of p38MAPK expression was examined...
12.
G蛋白调节子16对胶质瘤C6细胞周期的调节 - 张丰(第四军医大学西京医院病理科); 章必成; 叶菁; 陈广生; 王莉; 林圣彩
目的:探讨G蛋白调节子 16(RGS16)对胶质瘤C6细胞周期的影响。方法:利用脂质体介导法将RGS16基因导入C6细胞中 ,在倒置显微镜下观察细胞形态变化和贴壁生长情况 ;免疫细胞化学法检测转染前后RGS16蛋白的表达情况 ;流式细胞仪检测转染pCMV 5 RGS16和 pCMV 5质粒后每隔 12h后的细胞周期变化。 结果:转染pCMV 5 RGS16质粒 2 4h后 3 0 .0 %细胞贴壁性降低 ,突起收缩 ,细胞变圆 ,72h之后细胞又恢复正常 ;RGS16蛋白的表达呈时相性 ,3 6h时表达率最高 (阳性率为13 .0 % ) ,72h表达终止 ;C6细胞的各期细胞比例变化与RGS16蛋白表达对应 ,在 3 6h时G1期比例从转染前的 70 .5 %降低到60 .2 % ,S期比例从 2 0 .9%增加到 3 4.9% ;在 48h时G1期增加到 76.2 % ,S期减少到 11.4% ;72h各期恢复到正常比例。对照组细胞转染前后形态变化不明显 ,RGS16蛋白表达阴性 ,细胞周期变化不明显。结论:RGS16能促进C6细胞周期的运行.
【英文摘要】 Objective To study the effect of regulator of G protein signaling 16(RGS16) on the cell cycle of glioma C6 cell.Methods pCMV5 RGS16 was transfected into C6 cells by lipofectin.The morphological and adhesive changes of the cells were observed under an inverted microscope.Expression of RGS16 was examined...
13.
p38MAPK抑制剂SB-202190对大鼠胶质瘤C6细胞的双重作用 - 张丰(第四军医大学西京医院病理科); 李青(第四军医大学基础部免疫学教研室); 曹云新; 王莉; 邵秋杰; 林圣彩
目的 探讨 p38MAPK抑制剂SB 2 0 2 190对C6细胞生物活性的影响。方法 采用透射电镜的方法和DNA琼脂糖凝胶电泳的方法观察SB 2 0 2 190诱导胶质瘤细胞的凋亡 ;应用流式细胞仪检测细胞周期变化和是否有凋亡发生。结果 浓度分别为 10、2 0、4 0 μmol·L-1的SB 2 0 2 190能促进C6细胞的细胞周期从G1期过度到S期 ;但 6 0 μmol·L-1的SB 2 0 2 190却诱导C6细胞凋亡 ,凋亡率与时间呈正相关 ,透射电镜观察结果显示 ,凋亡细胞的体积缩小、细胞核浓聚缩小 ,染色质固缩聚集于核膜下 ,胞质浓缩 ,有的细胞染色质浓缩至核膜下成新月状 ;琼脂糖凝胶电泳呈现出特征性的DNA“梯状”带。结论 SB 2 0 2 190在低浓度时促进C6细胞增殖 ,高浓度时诱导细胞凋亡。
[英文摘要] AIM To study the effect of the specific inhibitor of p38MAPK,SB 202190, on the biological characteristics of glioma C6 cells. METHODS The morphological and adhesive changes of the cells were observed under an inverted microscope and C6 cells undergoing apoptosis were determineed by electron microscope and agrose gel electrophoresis after treatment of SB 202190. Flow Cytometry was adopted to measure the...
14.
p38MAPK和RGS16对大鼠胶质瘤C6细胞凋亡和细胞周期的影响 - 张丰(第四军医大学西京医院病理科); 李青(第四军医大学西京医院病理科); 王映梅(第四军医大学西京医院病理科); 王莉(第四军医大学西京医院病理科); 林圣彩
目的 探讨p38MAPK和RGS16对大鼠胶质瘤C6细胞的生物学特性的影响 .方法 利用脂质体介导法将p38MAPK和RGS16基因分别或共转染导入C6细胞中 ;用p38MAPK抑制剂SB 2 0 2 190处理处于对数生长期的转染和未转染 p38MAPK的C6细胞 ,2 4～ 36h后在倒置显微镜下观察细胞形态变化和贴壁情况 ;免疫细胞化学法检测转染前后p38MAPK和RGS16蛋白的表达情况 ;流式细胞仪检测细胞周期变化和细胞是否有凋亡发生 .结果 转染 pCMV5 p38和 /或pCMV5 RGS16质粒 36h后 30 %细胞贴壁性降低 ,突起收缩 ,细胞变圆 ;p38MAPK和RGS16蛋白均表达阳性 ;转染pCMV5 p38组出现 33.8%的凋亡峰 ,细胞周期结果显示G1期细胞百分数增加 17% ,而S期细胞百分数减少 14 % ;转染pCMV5 RGS16组无凋亡发生 ,细胞周期结果显示G1期细胞百分数减少 10 % ,而S期细胞百分数增加 14 % ;共转染pCMV5 P38和pCMV5 RGS16组未出现的凋亡峰 ,细胞周期结果显示G1期和S期细胞百分数变化与未处理组之间没有明...
【英文摘要】 AIM To study the effects of p38MAPK and RGS16 on the biological characteristics of glioma C6 cells. METHODS pCMV5 p38 and pCMV5 RGS16 were respectively or jointly transfected into C6 cells by lipofectin. SB 202190 was used to treat the transfected and nontransfected pCMV5 p38 C6 cells. The morphological and adhesive changes of the cells were...
16.
过度表达G蛋白调节子16促进大鼠胶质瘤C6细胞增殖 - 张丰(第四军医大学西京医院病理科); 李青(第四军医大学基础部病理学教研室; 章必成(第四军医大学西京医院病理科); 叶菁(第四军医大学西京医院病理科); 陈广生(第四军医大学西京医院病理科); 王莉(第四军医大学西京医院病理科); 林圣彩
目的 探讨 G蛋白调节子 16 (RGS16 )对大鼠胶质瘤C6细胞的生物学特性的影响 .方法 利用脂质体介导法将RGS16基因导入 C6细胞中 ,在倒置显微镜下观察细胞形态变化和贴壁情况 ;3H- Td R法检测 C6细胞在转染不同梯度p CMV5 - RGS16和 p CMV 5质粒后的增殖情况 ;免疫细胞化学法检测转染前后 RGS16蛋白的表达情况 ;流式细胞仪检测转染 p CMV5 - RGS16和 p CMV5质粒 36 h后细胞周期变化和细胞是否有凋亡发生 .结果 转染 p CMV5 - RGS16质粒 2 4 h后 30 %细胞贴壁性降低 ,突起收缩 ,细胞变圆 ;RGS16蛋白表达阳性 ;3H- Td R法检测显示 C6细胞增殖速度与转染p CMV5 - RGS16的量呈正相关 ;细胞周期结果显示 G1期细胞百分数减少 10 % ,而 S期细胞百分数增多 14 % ;未发现RGS16与凋亡有直接关系 .结论 RGS16可能促进 C6细胞的增殖 .
【英文摘要】 s: AIM To study the effect of RGS16 on the biological characteristics of glioma C6 cells. METHODS pCMV5 RGS16 was transfected into C6 cells by lipofectin. The morphological and adhesive changes of the cells were observed under an...
17.
p38MAPK基因诱导胶质瘤细胞凋亡 - 章必成; 李青(第四军医大学基础部病理学教研室; 巩西启; 叶菁; 陈广生; 王映梅; 林圣彩
目的 研究 p38MAPK基因转染大鼠胶质瘤细胞系C6后对其生物学特性的影响 .方法 利用脂质体介导法将p38MAPK基因导入大鼠胶质瘤细胞系 C6中 ,用免疫细胞化学染色检测其在细胞转染前后的表达情况 ,用 HE染色、流式细胞仪等方法研究其对细胞形态、粘着状况和生长周期的影响 .结果 转染 p CMV5 - p38MAPK质粒组 p38MAPK蛋白表达阳性 ,细胞形态发生变化 ,贴壁性降低 ,出现大量凋亡细胞 .结论 转染 p38MAPK基因可诱导胶质瘤细胞凋亡
【英文摘要】 AIM To study the effect of p38MAPK transfection on the biological characteristics of glioma cell C6. METHODS p38MAPK was transfected into glioma cell C6 by lipofectin. Expression of p38MAPK was detected by immunocytochemistry. HE staining and flow cytometry were adopted to measure the cell morphology, adhesion and cell cycle. RESULTS p38MAPK was expressed in transfected glioma cells, with cell biological characteristics changing and apoptotic cells emerging. CONCLUSION Apoptosis of glioma cell could be ...
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人工红树林幼林藤壶危害及防治研究进展 - 向平; 杨志伟; 林鹏
随着近年来红树林恢复性造林面积的扩大,海洋污损生物藤壶对红树林幼林的危害问题日益突出.文中综述了藤壶附着的生物化学、藤壶在红树林附着的生态学、藤壶对人工红树林幼林的危害和国内所采用的化学药物防治措施等方面的研究进展,以及今后的研究方向.藤壶在红树林的附着和分布模式受海水盐度、浸淹深度、林分郁闭度、水文条件等环境因素和生物因素的影响.而藤壶胶粘蛋白的氨基酸组成、一维结构,胶粘蛋白在水下的交联、组装和胶粘的过程与机制,以及藤壶危害红树幼苗的机制和危害权重尚需要深入探讨.研究红树植物对藤壶附着的响应和长期适应机制将为藤壶的防治提供更多的启示.
【英文摘要】 With the increasing area of restored mangrove vegetation, marine-fouling organisms, barnacle in particular, are suggested to be an important factor affecting the survival and growth of mangrove seedlings. This paper reviewed the biochemical and ecological studies on the settlement of barnacle, its damage on mangrove seedlings, and its chemical control. The settlement and distribution model of barnacle on mangroves is significantly affected by the environmental factors such as seawater salinity, tide inundat...
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木荷与杉木人工林枯枝落叶层水文生态功能 - 郭剑芬(福建师范大学); 杨玉盛; 林鹏
对福建建瓯木荷和杉木人工林枯枝落叶层截留降雨及保水保土功能的研究表明,20(处理1)、30(处理2)、40g(处理3)的原状枯枝落叶截留降雨量,杉木人工林比木荷人工林分别增加16.55%、16.26%、21.17%。在2002年1—12月不同降雨情况下,林内和林外微型小区枯枝落叶层减少径流量和泥沙量的效应均十分显著(P<0.05)。木荷和杉木人工林林内地表清除枯枝落叶层后产生的地表年径流深分别为385.62、682.76mm,是保留枯枝落叶层的1.61、1.79倍;泥沙流失量分别为668.95、1116.70kg·hm-2,是保留枯枝落叶层的2.46、2.52倍。林外微型小区覆盖150(处理Ⅰ)、300(处理Ⅱ)、600g(处理Ⅲ)木荷原状枯枝落叶产生的地表径流深分别为519.47、417.92、351.43mm,为对照的59.34%、47.74%、40.15%;泥沙量分别为2757.07、1746.33、936.66kg·hm-2,为对照的24.81%、15.71%、8.43%。
【英文摘要】 The eco-hydrological functions of forest floors in terms of rainfall interception and soil and water conservation were studied in Schima superba (SS) and Cunninghamia lanceolata (CF) plantations in Jian′ou, Fujian Province. Result showed that there are distinct differences in rainfall interception among three treatments in the two plantations. Rainfall interceptions for treatment 1, 2 and 3 in the CF plantation were 16.55%, 16.26% and 21.17% higher than those in the SS plantation, respectively. Simulation e...
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福建漳江口红树林区秋茄幼苗生长动态 - 张宜辉; 王文卿; 吴秋城; 方柏州; 林鹏
通过福建漳江口红树林自然保护区内8个样方24个小样方人工种植600个秋茄胚轴,在3a时间内对秋茄胚轴建立、幼苗生长以及环境因子进行定期观测。研究结果表明:林缘空地的秋茄生长状况良好,白骨壤林内最不利于秋茄幼苗的生长。潮位、盐度、底质土壤理化因子不是造成该样地各样方间秋茄幼苗生长差异的主要原因。动物取食、光照状况以及种间竞争是限制秋茄生长的主要环境因子。秋茄胚轴在长根前易于随潮水漂走,底质土壤中白骨壤致密的根系抑制了秋茄胚轴的定植,导致白骨壤林内秋茄幼苗漂走的数量最多。昆虫和螃蟹等动物的取食是导致林内已经固着生长的秋茄幼苗大量死亡的最主要原因,而林外被取食的幼苗个体极少。此后秋茄幼苗能否继续成长,主要取决于幼苗所接受到的光照条件。3a后,在荫蔽的树冠下,秋茄幼苗无法存活;而在林外,秋茄幼苗已经长成幼树。在林外滩涂上迅速生长的互花米草,也将影响秋茄幼苗的更新和生长。
【英文摘要】 To evaluate the establishment and early growth of the mangrove species Kandelia candel in the intertidal zone,and to develop a better understanding of biotic and abiotic factors influencing the regeneration of its seedlings,we conducted a field experiment in Zhangjiang Estuary in Fujian.Different positions along the intertidal gradient were selected from 20 m to 120 m horizontal distance down the shore,including eight sampling sites in the mangrove areas.Equal numbers(75) numbers of mature propagules of K.c...
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