3.
Gonad maturating rhythm and mating rate in Litopenaeus vannamei broodstock - 林琼武; 艾春香; 李少菁; 王桂忠; 陈学雷; 沈绿珠
在水温(27±2)℃、盐度29±2、pH 7.8~8.5、光强1 000 lx的条件下,对来自中国台湾省的体质量为35~45 g的凡纳滨对虾(Litopenaeus vannameiBoone 1931)亲虾的性腺成熟节律和交配进行了为期73 d的繁育实验,样品为1 300对,3个批次。结果表明,雌虾术后卵巢成熟率分4期:Ⅰ期(1~9 d)入池适应术后效应期;Ⅱ期(10~16 d)低成熟率期,Ⅲ期(17~51 d)成熟率渐上升期,Ⅳ(52~73 d)平稳期;雄虾精荚形成率分3期:Ⅰ期(1~12 d)入池适应与术后效应期;Ⅱ期(13~51 d)平缓上升期,Ⅲ期(52~73 d)平稳期。在95%的概率保证程度下,雌亲虾卵巢成熟率为9.73%,波动区间9.14%~11.53%;雄虾精荚形成率6.63%,波动区间5.87%~7.38%;平均交配率为61.43%,波动区间58.85%~64.00%。在繁殖过程中,雌虾卵巢再成熟时间间隔9.68 d,可多次成熟的次数19.49次,雄虾精荚再形成时间15.10 d,可多次形成的次数为12.50次。此外还就影响雌虾卵巢成熟和雄虾精荚形成的因素以及如何提高交配率进行了详细的讨论...
【英文摘要】 Beginning in 1988,Litopenaeus vannamei was introduced into China,and 1998 on a commercial scale.China now has a large and flourishing industry for Litopenaeus vannamei,with Mainland China producing more than 270?000 metric tonnes in 2002,300?000 metric tonnes(71 percent of the country's total shrimp production) in 2003,and 367?500 metric tonnes(78.5 percent of the country's total shrimp production) in 2004,which is higher than the current production of the whole of the Americas.The intensive culture of Lito...
5.
ULTRASTRUCTURE OF OOGENESIS IN THENUS ORIENTALIS - 朱冬发(宁波大学生命科学与生物工程学院); 王桂忠; 李少菁
根据卵细胞的形态、内部结构特征及卵母细胞与滤泡细胞之间的关系,东方扁虾的卵子发生可划分为卵原细胞、卵黄发生前卵母细胞、卵黄发生卵母细胞和成熟卵母细胞等四个时期。卵原细胞胞质稀少,胞器以滑面内质网为主。卵黄发生前卵母细胞核明显膨大,特称为生发泡;在靠近核外膜的胞质中可观察到核仁外排物。卵黄发生卵母细胞逐渐为滤泡细胞所包围;卵黄合成旺盛,胞质中因而形成并积累了越来越多的卵黄粒。东方扁虾卵母细胞的卵黄发生是二源的。游离型核糖体率先参与内源性卵黄合成形成无膜卵黄粒。粗面内质网是内源性卵黄形成的主要胞器。滑面内质网、线粒体和溶酶体以多种方式活跃地参与卵黄粒形成。卵周隙内的外源性物质有两个来源:滤泡细胞的合成产物和血淋巴携带、转运的卵黄蛋白前体物。这些外源性物质主要通过质膜的微吞饮作用和微绒毛的吸收作用这两种方式进入卵母细胞,进而形成外源性卵黄。内源性和外源性的卵黄物质共同参与成熟卵母细胞中富含髓样小体的卵黄粒的形成。卵壳的形成和微绒毛的回缩被认为是东方扁虾卵母细胞成熟的形态学标志。
【英文摘要】 Oogenesis and oocyte vitellogenesis in the flathead lobster Thenus orientalis were observed in detail by transmission electron microscopy.According to the shape and size of ootids,ultrastructural changes of nucleus and cytoplasm,vitelline accumulation and the relationship between oocytes and follicle cells,the oogenesis of T.orientalis could be divided into four stages,i.e.oogonia, previtellogenic oocytes,vitellogenic oocytes and mature oocytes.Besides a large and oval nucleus,the oogonia mainly contain ves...
6.
In vitro study of neuroendocrine regulation over the testicular development in mud crabs Scylla serrata - 叶海辉; 黄辉洋; 李少菁; 王桂忠; 李祺福
The reproductive endocrine activities of neuroendocrine organs and androgenic glands (AG) in male Scylla serrata were investigated with co-incubation technology. In vitro studies show that: (1) the AG in Stage Ⅲ can significantly accelerate the development of seminiferous tubules and spermic matu- ration; (2) the brain, thoracic ganglia and optic ganglia have no direct influence over the testicular devel- opment; (3) the brain and thoracic ganglia can significantly promote the growth of the AG cells and hig...
7.
凡纳滨对虾亲虾性腺成熟节律和交配率 - 林琼武; 艾春香; 李少菁; 王桂忠; 陈学雷; 沈绿珠
在水温(27±2)℃、盐度29±2、pH 7.8~8.5、光强1 000 lx的条件下,对来自中国台湾省的体质量为35~45 g的凡纳滨对虾(Litopenaeus vannameiBoone 1931)亲虾的性腺成熟节律和交配进行了为期73 d的繁育实验,样品为1 300对,3个批次。结果表明,雌虾术后卵巢成熟率分4期:Ⅰ期(1~9 d)入池适应术后效应期;Ⅱ期(10~16 d)低成熟率期,Ⅲ期(17~51 d)成熟率渐上升期,Ⅳ(52~73 d)平稳期;雄虾精荚形成率分3期:Ⅰ期(1~12 d)入池适应与术后效应期;Ⅱ期(13~51 d)平缓上升期,Ⅲ期(52~73 d)平稳期。在95%的概率保证程度下,雌亲虾卵巢成熟率为9.73%,波动区间9.14%~11.53%;雄虾精荚形成率6.63%,波动区间5.87%~7.38%;平均交配率为61.43%,波动区间58.85%~64.00%。在繁殖过程中,雌虾卵巢再成熟时间间隔9.68 d,可多次成熟的次数19.49次,雄虾精荚再形成时间15.10 d,可多次形成的次数为12.50次。此外还就影响雌虾卵巢成熟和雄虾精荚形成的因素以及如何提高交配率进行了详细的讨论...
【英文摘要】 Beginning in 1988,Litopenaeus vannamei was introduced into China,and 1998 on a commercial scale.China now has a large and flourishing industry for Litopenaeus vannamei,with Mainland China producing more than 270?000 metric tonnes in 2002,300?000 metric tonnes(71 percent of the country's total shrimp production) in 2003,and 367?500 metric tonnes(78.5 percent of the country's total shrimp production) in 2004,which is higher than the current production of the whole of the Americas.The intensive culture of Lito...
8.
锯缘青蟹生殖肢腺体的结构与功能 - 管卫兵; 王桂忠; 李少菁; 林琼武; 孔详会; 谭树华
青蟹雄性腹肢具有生殖肢腺体(GTG).雄性第一腹肢向末端逐渐变成一细薄的弯曲突起.腺体主要集中于腹肢距基部长度的2/5处,即第二腹肢入口处的附近,末端也有少量分布.腺体在组织学上和玫瑰状的皮腺相似,群绕着射精管腔.经染色表明腺体含有中性粘多糖(NMPS)和酸性粘多糖(AMPS),同一批腺体,甚至同一个腺体两种类型糖类的染色状况有异.腺体由分泌细胞和管细胞组成,管细胞含有一导管,多个导管形成网络.导管末端通向交接器内壁表皮开孔处,孔径约为2.5~3.5μm,这些孔通向交接器管腔,孔中及开口处分泌物的染色性质和腺体中相似.第二腹肢也具有腺体,但分布较少,且不太集中.GTG分泌物可能具有几个复杂的功能,其中低粘度的NMPS可能有助于加速强粘性精液通过狭窄的交接器管腔作用,而高粘度的AMPS功能则更为复杂.
【英文摘要】 The gonopod tegumental glands(GTG) of Scylla serrata grouped about the ejaculatory canal.The first gonopod tapered quickly to a thin,curved process terminating in a point.Tegumental glands were located at approximately 2/5 of the gonopod length,also some situated much further between approximately 3/5 or 4/5 of the gonopod length.GTG resembling the PTG(pleopod tegumental glands).The same group glands or a single gland show different intensely staining neutral mucopolysaccharide(NMPS) and an intensely staini...
9.
EVALUATION ON NUTRITIONAL COMPOSITION OF THE SOFT PART IN NACELLA CONCINNA OF GREAT WALL BAY, ANTARCTICA - 戴聪杰; 何剑锋; 王桂忠; 郑淑娴; 李少菁
采用常规的生化分析方法对南极帽贝软体部分的营养成分进行测定。结果表明:南极帽贝软体部分(干样)中粗蛋白含量为58.39%,粗脂肪含量为11.54%,灰分含量为9.97%;氨基酸总量为27.51%,必需氨基酸含量11.25%,占氨基酸总量的40.89%,第一限制氨基酸为缬氨酸,必需氨基酸指数为45.79;三种不饱和脂肪酸占脂肪酸总量的21.20%;矿质元素含量丰富。综合分析认为南极帽贝能为其捕食者如黑背鸥和企鹅提供优质的食物来源,同时也有一定的开发利用价值。
【英文摘要】 The limpet samples (Nacella concinna) were collected in the intertidal zone of Great Wall Bay, near the Great Wall Station, Antarctica, and analyzed by conventional biochemical methods. The result showed that the content of crude protein, lipid and ashes in the soft part (dry basis) was 58.39%, 11.54% and 9.97%, respectively. The content of the amino acids to the total dry weight of the soft part was 27.51%, in which the essential amino acids was 11.25%, accounting for 40.89 % of total amino acids. The EAAI...
10.
A study on temperature affect the energy budget of Haliotis diversicolor aquatilis - 阎希柱(集美大学水产学院); 王桂忠; 李少菁; 吕哲非
在摸拟自然条件下,设定5个温度梯度(15、19、23、27、31℃)进行了室内水族箱养殖,结果表明,九孔鲍(Haliotis diversicolorsuperrtesxa)的生长能和代谢能随温度的增加而增加,高于此温度时则相反,呈负相关关系。在温度为27℃时,九孔鲍的生长能占摄食能的比例达到最大值。九孔鲍在各温度下的能量收支方程为:15℃:100(C)=18.05(F)+8.00(U)+7.91(M)+39.60(R)+7.14(Psh)+9.36(Pg)+9.9419℃:100(C)=19.89(F)+12.24(U)+7.97(M)+33.74(R)+6.62(Psh)+9.54(Pg)+10.0123℃:100(C)=17.43(F)+12.93(U)+7.04(M)+33.79(R)+6.20(Psh)+10.25(Pg)+12.3727℃:100(C)=15.56(F)+11.03(U)+7.05(M)+28.53(R)+13.67(Psh)+16.12(Pg)+8.0331℃:100(C)=18.82(F)+13.87(U)+12.52(M)+38.44(R)+12.35(Psh)+13.62(...
【英文摘要】 Based on the study on the culture of Haliotis diversicolor aquatiliis with the different temperatures(15,19,23,27,31℃) under simulated natural conditions,the experimental data showed that the individual grow energy(P=Psh+Pg) and metabolism energy(R) could increase with the increase of temperature,but the negative relation occurred at higher temperature.The ratio of Grow energy(P) to Consumption energy(C) of Haliotis diversicolor aquatilis could get the peak at 27℃.The relationship between feces(F),excretion...
11.
The artificial simulation of sperm plug of Scylla serrta - 管卫兵; 王桂忠; 李少菁; 林琼武; 孔详会; 谭树华
采用模拟人工授精、组织学及电子显微镜观察等方法,研究了青蟹精子塞的形成机制。结果发现,青蟹精子塞为雄性精液和雌性纳精囊开口处的分泌物在短时间内发生剧烈反应的产物,它们在短时间内从液态变为胶状结构,证明了精子塞是雌雄蟹的共同产物。雌雄蟹形成的精子塞的作用是,将精液颗粒、精荚等紧密联系在一起,部分精荚被拉伸破裂;有助于防止后续的雌雄交配,保护或保持精荚的完整及具有营养作用。
【英文摘要】 During artifical transfer of spermatophore,the male seminal products and the female spermathecae secretion will occur a intense reaction in several seconds.As a result,the reaction products became a hard and gelatinous form which change strongly from the original liquid state.This product is the sperm plug.The phenomenon proves that the sperm plug of Scylla serrata is the common product of male and female crab.It 's possible to determined the role of either male or female crabs in the formation of the sperm...
12.
低温驯化下锯缘青蟹肝胰腺蛋白质表达及脂肪酸组成的变化 - 孔祥会(河南师范大学生命科学学院); 王桂忠; 王克坚; 李少菁
锯缘青蟹(Scylla serrata)在不同低温下经3周驯化后,采用双向电泳(Two division electrophoresis,2D-E)和气相色谱(Gas chromatography,GC)分别对肝胰腺中蛋白质表达和脂肪酸组成变化进行研究,结果发现5℃驯化组与27℃组相比,肝胰腺中共有22种蛋白的变化,其中5℃驯化组有15种蛋白在27℃组没有检出,而27℃组有7种蛋白在5℃驯化组没有发现;10℃驯化组与27℃组相比,共有18种蛋白变化,10℃驯化组有14种蛋白在27℃组没有检出,27℃组有4种蛋白在10℃驯化组没有出现;锯缘青蟹15℃驯化组与27℃组相比,肝胰腺中有6种蛋白质有变化,其中驯化组分离出5种在27℃组没有检出的蛋白质,27℃组分离出1种在驯化组没有出现的蛋白质。27℃组没有检出,仅在驯化组中出现的蛋白质中,经Imagemaster2D Elite软件分析后发现5℃和10℃驯化组有14种蛋白质在图谱上是匹配的(即是同样的),15℃驯化组表达的5种蛋白与5℃驯化组和10℃驯化组表达的蛋白不匹配。低温驯化3周后锯缘青蟹肝胰腺中饱和脂肪酸∑SFA在5℃和10℃驯化组显著低于27℃组(P<0....
【英文摘要】 Mud crab, Scylla serrata , is an economic crustacean living in estuarine waters. Studies on mechanism of low temperature adaptation are important for low temperature breeding,as well as crab aquiculture. Two-division electrophoresis, including isoelec-trophoresis focusing (IEF) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ,was important method for proteins division in proteomes, which was used to determine expression of specific proteins in hepatopancreas of Scylla serrata under ...
13.
Acrosin Activity of Spermatozoa in Spermathecae of Scylla serrata - 管卫兵; 王桂忠; 李少菁; 陈锦民
顶体酶是精子顶体部特有的胰蛋白酶,其在顶体反应、精子和透明带的结合及穿透中起重要作用。本文采用测定精子顶体氨基化酶活力方法来测定锯缘青蟹(Scyllaserrata)精子的顶体酶活力。据测定,青蟹纳精囊精子顶体酶活力分别为3.566μIU/106和4.248μIU/106。青蟹精子顶体酶活力和用同样方法测定的其它物种(如猪、人、马)相比较低。这反应了不同物种之间的精子顶体酶活力存在着较显著的差异,也反应青蟹纳精囊中精子处于一种抑制状态。这种精子活力抑制方式可能有利于精子在纳精囊中的长期存活。本实验结果也说明,临床应用的顶体酶测定方法也可以用于蟹类精液质量的评价。
【英文摘要】 Acrosin, a sperm-specific arosomal proteinase, has an essential role in the fertilization process and is thought to be important in the acrosome reaction, sperm-zona binding and zona penetration. The spermatozoa acrosin activity of Scylla serrata is measured with a simplified clinical assay of total sperm acrosin activity. This method is based on the measurement of amidase activity of sperm suspensions. The acrosin activity assay has been used in many animals. Results of these studies indicated that use of ...
14.
不同饵料、密度和池底对锯缘青蟹大眼幼体蜕皮变态的影响 - 徐晓群; 朱小明; 费亮亮; 王桂忠; 李少菁
实验观察了不同饵料、池底、密度对锯缘青蟹大眼幼体蜕皮变态率和残杀率的影响.实验结果表明:投喂裸腹蚤时,大眼幼体蜕皮变态率最高,3种池底大眼幼体蜕皮变态率平均为 78.1%,残杀率最低平均为 11.4%;海泥池底大眼幼体蜕皮变态率最高,投喂3种饵料的大眼幼体蜕皮变态率平均为84.8%,残杀率最低平均为7.6%;裸腹蚤是青蟹大眼幼体培育适宜的饵料,而海泥则是大眼幼体变态适宜的池底.不同培育密度对青蟹大眼幼体蜕皮变态率和残杀率的实验结果表明:当大眼幼体的培育密度为3 尾/dm3 时,其蜕皮变态率最高,而残杀率与大眼幼体培育密度的关系不明确.
【英文摘要】 Effects of different dietary,densities and substrata on metamorphosis rate and cannibalistic rate from protozoea 1 (PZ1) to postlarvae 1(PL1) stages of Scylla serrata megalopae were studied in two separate experiments.In the first experiment,three dietary:Monia mongolica,minced meat of Ruditapes philippinaram which filted through mesh and haima 0 shrimp stuff and three substrata:sea mud,sand and cement were investigated.It showed that the metamorphosis rate of megalopae was higher when it was fed Monia mong...
15.
The experimental studies on the carbon and nitrogen budgets in Pseudeuphausia sinica - 郭东晖; 李少菁; 陈峰; 王桂忠; 陈钢
实验室培养条件下,以新近孵化的卤虫(Artemiasalina)为食物,观测中华假磷虾(Pseudeuphau siasinica)雌性成体、仔虾和后期带叉幼体的碳氮收支.实验表明摄食率随食物浓度的升高呈线性提高,成体未出现饱和现象,反映其较高的摄食潜力.呼吸率(碳消耗率)与碳的摄食率及同化率分别呈线性正相关,比动力作用分别为9% ~16% (摄入碳)和10% ~17% (同化碳)以后期幼体更小.总氮排泄率与氮的摄食率及同化率也分别呈显著正相关,但仔虾例外.排粪率随摄食率升高而提高,从而同化效率与摄食率无关,介于0 84~0 95.食物氮源是中华假磷虾生长的主要限制因子,当摄食率低于10μg/(mg·d) (干重)时,假磷虾呈负生长.同化的氮以排泄消耗为主,体质生长次之,而蜕皮损失最少.同化的碳也以呼吸消耗和体质生长居多,但随摄食率有变化,蜕皮损失最少.在成熟雌体,估算的生殖生长(碳、氮)比蜕皮损失略高.净生长效率随摄食率(及同化率)升高而升高.达一定值后,仔虾和后期带叉幼体净生长效率略有下降.
【英文摘要】 The carbon and nitrogen budgets are estimated on the adult females, juveniles and post-furcilia larvae of Pseudeuphausia sinica in the laboratory with newly hatching nauplii of Artemia salina as food.It is found that the ingestion rate is linearly related to the food concentration, suggesting high feeding potential.The linear correlation can be established between the respiration rate (carbon consumption rate ) and carbon ingestion rate, as well as carbon assimilation rate.The regression coefficients ( i.e....
16.
Microstructure and ultrastructure of mandibular organ in the mud crab, Scylla serrata - 叶海辉; 黄辉洋; 李少菁; 王桂忠
采用组织切片和电镜技术,对锯缘青蟹大颚器的形态结构进行了观察.大颚器位于大颚肌几丁质腱的后侧基部.腺细胞胞质丰盈,质膜大量内陷和作不同程度的卷绕,内含大量具管状脊的线粒体和滑面内质网.卵巢成熟时大颚器腺细胞内线粒体增多,少数线粒体形态异样,高尔基体易见,散布的小管状滑面内质网(tSER)少,高度汇合的tSER增多,大小形状不同的空泡数量明显增加,且近质膜分布,质膜内陷和卷绕程度加剧.表明此时锯缘青蟹大颚器的合成与分泌能力增强,其生理功能与卵巢发育密切相关.
【英文摘要】 The paired mandibular organs are located at the base of the mandibular tendon. The plasma membrane of the glandular cells is convoluted, especially at the adjaction to the hemolymph channels. The cytoplasm has numerous smooth endoplasmic reticulum and mitochondria with tubular cristae. In the maturation stage of the ovaries, the mitochondria grow up in number, a few of which change morphologically; the developed Golgi complexes are frequently observed; the scattered tSER reduces, while the highly converged ...
17.
Immunocytochemical studies on the optic ganglia of the mud crab, Scylla serrata (Forskl) - 黄辉洋; 李少菁; 叶海辉; 王桂忠; 李祺福
采用兔抗哺乳类血清,应用链霉菌抗生物素蛋白-过氧化物酶免疫细胞化学技术,观察了锯缘青蟹视神经节中5 羟色胺、胰高血糖素、神经肽Y和生长抑素免疫阳性细胞和神经纤维的形态和分布,结果发现,视神经节的4个神经髓均有5 羟色胺免疫阳性细胞,除X器检出1个较大型阳性细胞外,其余均为小型细胞.视外髓、视内髓和视端髓都具有胰高血糖素免疫阳性细胞,视端髓的神经髓质阳性染色深,X器中阳性细胞成群分布,窦腺免疫阳性反应强.神经肽Y免疫阳性细胞在视神经层和视外髓为小型阳性细胞,在视内髓和视端髓检出较大型阳性细胞,另有小型阳性细胞散布于X器中.生长抑素免疫阳性细胞分布于4个神经髓,数量少. 4种免疫阳性物的特异性分布模式,可为其不同的神经生理作用提供形态学证据.
【英文摘要】 s: Immunocytochemistry technologies were applied to observe the immunoreactive neurons and neuropils of serotonin (5-HT), glucagon (Glu), neuropeptide Y (NPY) and somatostatin (SOM) in the optic ganglia of Scylla serrata. The results are as follows: 5-HT-immunoreactive cells are located in lamina ganglionaris(LG), medulla externa(ME), medulla interna(MI)and medulla terminalis(MT). Except one large immunoreactive cell in the X organ, the other immunoreactive cells are small. The presence of Glu-immunoreactiv...
18.
Direct connection between androgenic glands and horacic ganglion of Scylla serrata - 管卫兵; 王桂忠; 李少菁; 林琼武; 孔详会; 谭树华
采用组织学、扫描电镜方法对锯缘青蟹(Scyllaserrata)促雄腺和胸腹神经团之间的关系进行观察。结果表明,射精管末端存在促雄腺,促雄腺和胸神经团来源的支配第五步足(即游泳足)的神经紧密相连。该神经在游泳足基部的肌肉内部紧贴射精管并行,其分支支配着射精管周围肌肉,可能也是控制精荚排放的神经。本研究可为促雄腺对性别控制的机制及蟹类精荚电促排的深入研究提供理论依据。
【英文摘要】 Androgenic gland hormone (AGH) is responsible for male sexual differentiation in crustaceans. An androgenic gland hormone may allow hormonal sex reversal for the production of monosex populations in aquaculture, leading to increases in production. There are many researches about the androgenic gland and its hormone by different research methods. Because AGH of male crab produces components of seminal fluid that alter the behavior, physiology and life span of mated female, and contribute to her efficient sto...
19.
Relationship between salinity tolerance during different developmental phase and ecological distribution of Schmackeria poplesia in Jiulongjiang Estuary in Fujian - 商栩; 王桂忠; 李少菁
利用驯化及世代培养,对生活于福建九龙江口低盐水域的火腿许水蚤的生理耐盐能力进行了研究.结果显示不同发育期的个体具有不同的生理耐盐范围,耐盐能力随着个体发育呈无节幼体<桡足幼体<成体的趋势增长.在盐度变化的环境中无节幼体的耐盐能力是决定其群体增殖量的主要因素,并进而影响其生态分布.盐度驯化能有效地扩大该种桡足类的耐盐范围,从而了解其潜在的地理分布范围.火腿许水蚤表现出较强的对高盐环境的潜在适应能力,但无法适应淡水环境.这表明其在该水域的生态分布状况不仅是生理耐盐能力的反映,种间竞争和河口水文条件也是造成这一现象的重要影响因素.
【英文摘要】 This study examined the physiological salinity tolerance of Schmackeria poplesia inhabited in the low salinity zone (LSZ) in the Jiulongjiang Estuary after acclimation and culturing for generations. The results indicate there are different salinity tolerances during development, and following the increasing tendency as nauplii < copepodites < adults. The salinity endurance of nauplii is vital to the population recruitment under salinity fluctuation, and then affects its ecological distribution. The salinity...
20.
锯缘青蟹精荚移植人工授精初探 - 管卫兵; 王桂忠; 李少菁; 林琼武; 孔详会; 谭树华
模仿雄性锯缘青蟹第一腹肢,根据其在交配中的作用机制,采用常规滴管为材料,利用酒精灯灼热拉制一个前端弯曲、侧扁、管径大小接近第一腹肢的人工交接器.以无钙离子的人工海水作为青蟹人工授精的缓冲液,缓冲液中加入适量庆大霉素.首次对青蟹采用类似硬壳交配的方式,将成熟雄蟹贮精囊内的精荚和精液成功移植至雌蟹纳精囊中.结果表明精荚的人工移植不会破坏成熟雌蟹纳精囊的内环境,其内精子具正常受精能力.未交配雌青蟹精荚的人工移植没有正常产卵,但性腺得以促进发育,并能进行正常的生殖脱皮.
【英文摘要】 Imitate male first gonopod of male mud crab, based on its function in reproduction and mate, an artificial mate organ is manufactured. The spermatophores are extended by the Ca~(2+)-FSW, gentamicin is also appended in the extender. With the similar hard carapace mate methods, the spermatophores and semen are transferred to spermathacae of female mud crab artificially. As a result, the mature female mud crab can spawn, though the number of eggs laid is affected. The transferring in unmated female may not spa...
1.
